Illuminating Enterovirus 71 RNA Replication Sites

a 2024

Illuminating Enterovirus 71 RNA Replication Sites

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK a Pavel PLEVKA

Základní údaje

Originální název

Illuminating Enterovirus 71 RNA Replication Sites

Autoři

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK a Pavel PLEVKA

Vydání

22nd meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC), 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Stát vydavatele

Finsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/24:00139133

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

enterovirus; HFMD; antiviral therapies; replication; fluorescent reporter; fluorescent microscope

Štítky

rivok

Změněno: 19. 3. 2025 14:36, Mgr. Eva Dubská

Anotace

V originále

Enterovirus 71 (EV71), a member of the Picornaviridae family, poses a significant threat as a cause of hand-foot-and-mouth disease (HFMD), primarily impacting small children. The potential for severe neurological complications, leading to mortality rates of up to 19%, highlights the need for research efforts to address this pathogen1. With the escalating outbreaks in the Asia-Pacific region, the exploration of novel therapeutic approaches is indispensable. While current vaccines target specific serotypes, their efficacy lacks evidence of cross-protection. This underscores the importance of investigating targeted antiviral therapies1.2. However, progress is hindered by gaps in understanding EV71's replication cycle. This study aims to examine the unmapped facets of enterovirus RNA replication, focusing on its interaction with genome packaging and viral assembly. A key challenge in unravelling the molecular intricacies of the replication process is the development of a fluorescent reporter system to visualise viral replication sites. We present the establishment of a fluorescent reporter that enables specific labelling of EV71 replication sites without altering the viral genome, hence preserving the natural course of genome replication. This system will serve as a crucial tool for guiding subsequent research phases, including correlative light and electron microscopy for precise lamella preparation and cryo-electron tomography data collection.

Návaznosti

EH22_008/0004607, projekt VaV

Název: Nové technologie pro translační výzkum ve farmaceutických vědách /NETPHARM

Citovat

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK a Pavel PLEVKA. Illuminating Enterovirus 71 RNA Replication Sites. In 22nd meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC). 2024.

@proceedings{2485303,
   author = {Nepovímová, Lucie and Füzik, Tibor and Plevka, Pavel},
   booktitle = {22nd meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC)},
   keywords = {enterovirus; HFMD; antiviral therapies; replication; fluorescent reporter; fluorescent microscope},
   language = {eng},
   title = {Illuminating Enterovirus 71 RNA Replication Sites},
   url = {https://www.jyu.fi/en/events/europic-2024},
   year = {2024}
}

TY  - CONF
ID  - 2485303
AU  - Nepovímová, Lucie - Füzik, Tibor - Plevka, Pavel
PY  - 2024
TI  - Illuminating Enterovirus 71 RNA Replication Sites
KW  - enterovirus
KW  - HFMD
KW  - antiviral therapies
KW  - replication
KW  - fluorescent reporter
KW  - fluorescent microscope
UR  - https://www.jyu.fi/en/events/europic-2024
N2  - Enterovirus 71 (EV71), a member of the Picornaviridae family, poses a significant threat as a cause of hand-foot-and-mouth disease (HFMD), primarily impacting small children. The potential for severe neurological complications, leading to mortality rates of up to 19%, highlights the need for research efforts to address this pathogen1. With the escalating outbreaks in the Asia-Pacific region, the exploration of novel therapeutic approaches is indispensable. While current vaccines target specific serotypes, their efficacy lacks evidence of cross-protection. This underscores the importance of investigating targeted antiviral therapies1.2. However, progress is hindered by gaps in understanding EV71's replication cycle. This study aims to examine the unmapped facets of enterovirus RNA replication, focusing on its interaction with genome packaging and viral assembly. A key challenge in unravelling the molecular intricacies of the replication process is the development of a fluorescent reporter system to visualise viral replication sites. We present the establishment of a fluorescent reporter that enables specific labelling of EV71 replication sites without altering the viral genome, hence preserving the natural course of genome replication. This system will serve as a crucial tool for guiding subsequent research phases, including correlative light and electron microscopy for precise lamella preparation and cryo-electron tomography data collection.
ER  -

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK a Pavel PLEVKA. Illuminating Enterovirus 71 RNA Replication Sites. In \textit{22nd meeting of the European Study Group on the Molecular Biology of Picornaviruses (EUROPIC)}. 2024.

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