Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound
FMN by Stimulated Raman and Visible Absorption Spectroscopies

J 2023

Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies

LIU, Yingliang; Aditya S CHAUDHARI; Aditi CHATTERJEE; Prokopis C ANDRIKOPOULOS; Alessandra PICCHIOTTI et al.

Základní údaje

Originální název

Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies

Autoři

LIU, Yingliang; Aditya S CHAUDHARI; Aditi CHATTERJEE; Prokopis C ANDRIKOPOULOS; Alessandra PICCHIOTTI; Mateusz REBARZ; Miroslav KLOZ; Victor A LORENZ-FONFRIA; Bohdan SCHNEIDER a Gustavo FUERTES

Vydání

Biomolecules, Basel, Switzerland, MDPI AG, 2023, 2218-273X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90127/23:00139141

Organizační jednotka

CIISB II

Klíčová slova anglicky

flavins; protein structural dynamics; light-oxygen-voltage (LOV) photosensors; femtosecond-stimulated Raman spectroscopy (FSRS); transient visible absorption (visTA) spectroscopy; time-resolved vibrational spectroscopy; entropy

Štítky

ne MU, rivok

Změněno: 19. 3. 2025 17:28, Mgr. Eva Dubská

Anotace

V originále

Time-resolved femtosecond-stimulated Raman spectroscopy (FSRS) provides valuable information on the structural dynamics of biomolecules. However, FSRS has been applied mainly up to the nanoseconds regime and above 700 cm(-1), which covers only part of the spectrum of biologically relevant time scales and Raman shifts. Here we report on a broadband (similar to 200-2200 cm(-1)) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump. The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222. The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments. Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm(-1). We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

LIU, Yingliang; Aditya S CHAUDHARI; Aditi CHATTERJEE; Prokopis C ANDRIKOPOULOS; Alessandra PICCHIOTTI; Mateusz REBARZ; Miroslav KLOZ; Victor A LORENZ-FONFRIA; Bohdan SCHNEIDER a Gustavo FUERTES. Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies. Biomolecules. Basel, Switzerland: MDPI AG, 2023, roč. 13, č. 1, s. 1-12. ISSN 2218-273X. Dostupné z: https://doi.org/10.3390/biom13010161.

@article{2485325,
   author = {Liu, Yingliang and Chaudhari, Aditya S and Chatterjee, Aditi and Andrikopoulos, Prokopis C and Picchiotti, Alessandra and Rebarz, Mateusz and Kloz, Miroslav and LorenzandFonfria, Victor A and Schneider, Bohdan and Fuertes, Gustavo},
   article_location = {Basel, Switzerland},
   article_number = {1},
   doi = {https://doi.org/10.3390/biom13010161},
   keywords = {flavins; protein structural dynamics; light-oxygen-voltage (LOV) photosensors; femtosecond-stimulated Raman spectroscopy (FSRS); transient visible absorption (visTA) spectroscopy; time-resolved vibrational spectroscopy; entropy},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies},
   url = {https://www.mdpi.com/2218-273X/13/1/161},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2485325
AU  - Liu, Yingliang - Chaudhari, Aditya S - Chatterjee, Aditi - Andrikopoulos, Prokopis C - Picchiotti, Alessandra - Rebarz, Mateusz - Kloz, Miroslav - Lorenz-Fonfria, Victor A - Schneider, Bohdan - Fuertes, Gustavo
PY  - 2023
TI  - Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies
JF  - Biomolecules
VL  - 13
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - MDPI AG
SN  - 2218273X
KW  - flavins
KW  - protein structural dynamics
KW  - light-oxygen-voltage (LOV) photosensors
KW  - femtosecond-stimulated Raman spectroscopy (FSRS)
KW  - transient visible absorption (visTA) spectroscopy
KW  - time-resolved vibrational spectroscopy
KW  - entropy
UR  - https://www.mdpi.com/2218-273X/13/1/161
N2  - Time-resolved femtosecond-stimulated Raman spectroscopy (FSRS) provides valuable information on the structural dynamics of biomolecules. However, FSRS has been applied mainly up to the nanoseconds regime and above 700 cm(-1), which covers only part of the spectrum of biologically relevant time scales and Raman shifts. Here we report on a broadband (similar to 200-2200 cm(-1)) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump. The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222. The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments. Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm(-1). We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
ER  -

LIU, Yingliang; Aditya S CHAUDHARI; Aditi CHATTERJEE; Prokopis C ANDRIKOPOULOS; Alessandra PICCHIOTTI; Mateusz REBARZ; Miroslav KLOZ; Victor A LORENZ-FONFRIA; Bohdan SCHNEIDER a Gustavo FUERTES. Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies. \textit{Biomolecules}. Basel, Switzerland: MDPI AG, 2023, roč.~13, č.~1, s.~1-12. ISSN~2218-273X. Dostupné z: https://doi.org/10.3390/biom13010161.

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