SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS
FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS

k 2024

SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS

BOBÁĽ, Pavel; Michal GREGUŠ; Hana PÍŽOVÁ; Denisa SMOLKOVÁ; Richard ČMELÍK et al.

Základní údaje

Originální název

SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS

Autoři

BOBÁĽ, Pavel; Michal GREGUŠ; Hana PÍŽOVÁ; Denisa SMOLKOVÁ; Richard ČMELÍK a Jana LAVICKÁ

Vydání

52th Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10406 Analytical chemistry

Utajení

není předmětem státního či obchodního tajemství

Odkazy

BOOK OF ABSTRACTS

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14160/24:00139148

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

MS; fluorescent labeling tags; fluorescence; oligosacharides

Štítky

rivok, ÚChL

Změněno: 21. 3. 2025 10:52, Ing. Richard Heger, Ph.D.

Anotace

V originále

Sample preparation is crucial for oligosaccharide and glycan analysis by chromatographic or electrophoretic methods. Since native oligosaccharides and glycans lack chromophores or fluorophores, they require molecular modification for optical detection. Adding a charged group also enhances electrophoretic mobility and ionization efficiency for MS detection. Standard oligosaccharides and N-linked glycans were derivatized with newly synthesized fluorescent and MS-active labels. After purification, the labeled compounds were separated by CE or LC and detected using fluorescence or MS. Novel labels based on 2-phenylpyridine derivatives were created, allowing tuning of fluorescence and MS properties. Compared to commercially available labels, these new labels showed higher reaction yields, tunable fluorescence, and better ionization efficiency. This work showcases the development of novel fluorescent labels with fast reaction kinetics, high fluorescence yields, and improved MS detectability.

Návaznosti

GA22-00236S, projekt VaV

Název: Vývoj nových fluorescenčních značek pro elektroforetické analýzy glykoproteinů asociovaných s rakovinou prsu (Akronym: 22-00236S)

Investor: Grantová agentura ČR, Vývoj nových fluorescenčních značek pro elektroforetické analýzy glykoproteinů asociovaných s rakovinou prsu

Citovat

BOBÁĽ, Pavel; Michal GREGUŠ; Hana PÍŽOVÁ; Denisa SMOLKOVÁ; Richard ČMELÍK a Jana LAVICKÁ. SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS. In 52th Conference SYNTHESIS AND ANALYSIS OF DRUGS. 2024.

@proceedings{2485493,
   author = {Bobáľ, Pavel and Greguš, Michal and Pížová, Hana and Smolková, Denisa and Čmelík, Richard and Lavická, Jana},
   booktitle = {52th Conference SYNTHESIS AND ANALYSIS OF DRUGS},
   keywords = {MS; fluorescent labeling tags; fluorescence; oligosacharides},
   language = {eng},
   title = {SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS},
   url = {https://www.faf.cuni.cz/getattachment/Special/SAL2024/Program/SAL_2024_Book_of_Abstracts_v2.pdf.aspx?lang=en-GB},
   year = {2024}
}

TY  - CONF
ID  - 2485493
AU  - Bobáľ, Pavel - Greguš, Michal - Pížová, Hana - Smolková, Denisa - Čmelík, Richard - Lavická, Jana
PY  - 2024
TI  - SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
KW  - MS
KW  - fluorescent labeling tags
KW  - fluorescence
KW  - oligosacharides
UR  - https://www.faf.cuni.cz/getattachment/Special/SAL2024/Program/SAL_2024_Book_of_Abstracts_v2.pdf.aspx?lang=en-GB
N2  - Sample preparation is crucial for oligosaccharide and glycan analysis by chromatographic or electrophoretic methods. Since native oligosaccharides and glycans lack chromophores or fluorophores, they require molecular modification for optical detection. Adding a charged group also enhances electrophoretic mobility and ionization efficiency for MS detection. Standard oligosaccharides and N-linked glycans were derivatized with newly synthesized fluorescent and MS-active labels. After purification, the labeled compounds were separated by CE or LC and detected using fluorescence or MS. Novel labels based on 2-phenylpyridine derivatives were created, allowing tuning of fluorescence and MS properties. Compared to commercially available labels, these new labels showed higher reaction yields, tunable fluorescence, and better ionization efficiency. This work showcases the development of novel fluorescent labels with fast reaction kinetics, high fluorescence yields, and improved MS detectability.
ER  -

BOBÁĽ, Pavel; Michal GREGUŠ; Hana PÍŽOVÁ; Denisa SMOLKOVÁ; Richard ČMELÍK a Jana LAVICKÁ. SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS. In \textit{52th Conference SYNTHESIS AND ANALYSIS OF DRUGS}. 2024.

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