Structural characterization and functional insights into the
type II secretion system of the poly-extremophile Deinococcus
radiodurans

J 2024

Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans

FARCI, Domenica; Stefan MILENKOVIC; Luca IESU; Marta TANAS; Matteo CECCARELLI et. al.

Základní údaje

Originální název

Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans

Autoři

FARCI, Domenica; Stefan MILENKOVIC; Luca IESU; Marta TANAS; Matteo CECCARELLI a Dario PIANO

Vydání

International Journal of Biological Chemistry, AMSTERDAM, ANSInet, 2024, 1819-155X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:90127/24:00139155

Organizační jednotka

CIISB II

DOI

https://doi.org/10.1016/j.jbc.2023.105537

UT WoS

001345623900001

EID Scopus

2-s2.0-85183024245

Klíčová slova anglicky

S-LAYER COMPLEX; VISUALIZATION; PREDICTION; PROTEINSUNIT; HPI; DNA

Štítky

CF CRYO, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2025 01:00, Mgr. Eva Dubská

Anotace

V originále

The extremophile bacterium D. radiodurans boasts a distinctive cell envelope characterized by the regular arrangement of three protein complexes. Among these, the Type II Secretion System (T2SS) stands out as a pivotal structural component. We used cryo-electron microscopy to reveal unique features, such as an unconventional protein belt (DR_1364) around the main secretin (GspD), and a cap (DR_0940) found to be a separated subunit rather than integrated with GspD. Furthermore, a novel region at the N-terminus of the GspD constitutes an additional second gate, supplementing the one typically found in the outer membrane region. This T2SS was found to contribute to envelope integrity, while also playing a role in nucleic acid and nutrient trafficking. Studies on intact cell envelopes show a consistent T2SS structure repetition, highlighting its significance within the cellular framework.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

FARCI, Domenica; Stefan MILENKOVIC; Luca IESU; Marta TANAS; Matteo CECCARELLI a Dario PIANO. Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans. International Journal of Biological Chemistry. AMSTERDAM: ANSInet, 2024, roč. 300, č. 2, s. 1-10. ISSN 1819-155X. Dostupné z: https://doi.org/10.1016/j.jbc.2023.105537.

@article{2485960,
   author = {Farci, Domenica and Milenkovic, Stefan and Iesu, Luca and Tanas, Marta and Ceccarelli, Matteo and Piano, Dario},
   article_location = {AMSTERDAM},
   article_number = {2},
   doi = {https://doi.org/10.1016/j.jbc.2023.105537},
   keywords = {S-LAYER COMPLEX; VISUALIZATION; PREDICTION; PROTEINSUNIT; HPI; DNA},
   language = {eng},
   issn = {1819-155X},
   journal = {International Journal of Biological Chemistry},
   title = {Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans},
   url = {https://www.jbc.org/article/S0021-9258(23)02565-6/fulltext},
   volume = {300},
   year = {2024}
}

TY  - JOUR
ID  - 2485960
AU  - Farci, Domenica - Milenkovic, Stefan - Iesu, Luca - Tanas, Marta - Ceccarelli, Matteo - Piano, Dario
PY  - 2024
TI  - Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans
JF  - International Journal of Biological Chemistry
VL  - 300
IS  - 2
SP  - 1-10
EP  - 1-10
PB  - ANSInet
SN  - 1819155X
KW  - S-LAYER COMPLEX
KW  - VISUALIZATION
KW  - PREDICTION
KW  - PROTEINSUNIT
KW  - HPI
KW  - DNA
UR  - https://www.jbc.org/article/S0021-9258(23)02565-6/fulltext
N2  - The extremophile bacterium D. radiodurans boasts a distinctive cell envelope characterized by the regular arrangement of three protein complexes. Among these, the Type II Secretion System (T2SS) stands out as a pivotal structural component. We used cryo-electron microscopy to reveal unique features, such as an unconventional protein belt (DR_1364) around the main secretin (GspD), and a cap (DR_0940) found to be a separated subunit rather than integrated with GspD. Furthermore, a novel region at the N-terminus of the GspD constitutes an additional second gate, supplementing the one typically found in the outer membrane region. This T2SS was found to contribute to envelope integrity, while also playing a role in nucleic acid and nutrient trafficking. Studies on intact cell envelopes show a consistent T2SS structure repetition, highlighting its significance within the cellular framework.
ER  -

FARCI, Domenica; Stefan MILENKOVIC; Luca IESU; Marta TANAS; Matteo CECCARELLI a Dario PIANO. Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans. \textit{International Journal of Biological Chemistry}. AMSTERDAM: ANSInet, 2024, roč.~300, č.~2, s.~1-10. ISSN~1819-155X. Dostupné z: https://doi.org/10.1016/j.jbc.2023.105537.

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