Orthotopic model for the analysis of melanoma circulating tumor
cells

J 2024

Orthotopic model for the analysis of melanoma circulating tumor cells

PICKOVÁ, Markéta, Zuzana KAHOUNOVÁ, Tomasz Witold RADASZKIEWICZ, Jiřina PROCHÁZKOVÁ, Radek FEDR et. al.

Základní údaje

Originální název

Orthotopic model for the analysis of melanoma circulating tumor cells

Autoři

PICKOVÁ, Markéta, Zuzana KAHOUNOVÁ, Tomasz Witold RADASZKIEWICZ, Jiřina PROCHÁZKOVÁ, Radek FEDR, Michaela NOSKOVA, Katarzyna Anna RADASZKIEWICZ, Petra OVESNA, Vítězslav BRYJA a Karel SOUČEK

Vydání

Nature Scientific Reports, BERLIN, NATURE PORTFOLIO, 2024, 2045-2322

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.800 v roce 2023

DOI

http://dx.doi.org/10.1038/s41598-024-58236-y

UT WoS

001273217900058

Klíčová slova anglicky

In vivo model; Melanoma; Circulating tumor cells; Metastasis; Tumorectomy

Změněno: 24. 3. 2025 10:00, Mgr. Jiřina Procházková, Ph.D.

Anotace

V originále

Metastatic melanoma, a highly lethal form of skin cancer, presents significant clinical challenges due to limited therapeutic options and high metastatic capacity. Recent studies have demonstrated that cancer dissemination can occur earlier, before the diagnosis of the primary tumor. The progress in understanding the kinetics of cancer dissemination is limited by the lack of animal models that accurately mimic disease progression. We have established a xenograft model of human melanoma that spontaneously metastasizes to lymph nodes and lungs. This model allows precise monitoring of melanoma progression and is suitable for the quantitative and qualitative analysis of circulating tumor cells (CTCs). We have validated a flow cytometry-based protocol for CTCs enumeration and isolation. We could demonstrate that (i) CTCs were detectable in the bloodstream from the fourth week after tumor initiation, coinciding with the lymph node metastases appearance, (ii) excision of the primary tumor accelerated the formation of metastases in lymph nodes and lungs as early as one-week post-surgery, accompanied by the increased numbers of CTCs, and (iii) CTCs change their surface protein signature. In summary, we present a model of human melanoma that can be effectively utilized for future drug efficacy studies.

Citovat

PICKOVÁ, Markéta, Zuzana KAHOUNOVÁ, Tomasz Witold RADASZKIEWICZ, Jiřina PROCHÁZKOVÁ, Radek FEDR, Michaela NOSKOVA, Katarzyna Anna RADASZKIEWICZ, Petra OVESNA, Vítězslav BRYJA a Karel SOUČEK. Orthotopic model for the analysis of melanoma circulating tumor cells. Nature Scientific Reports. BERLIN: NATURE PORTFOLIO, 2024, roč. 14, č. 1, 13 s. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-024-58236-y.

@article{2486358,
   author = {Picková, Markéta and Kahounová, Zuzana and Radaszkiewicz, Tomasz Witold and Procházková, Jiřina and Fedr, Radek and Noskova, Michaela and Radaszkiewicz, Katarzyna Anna and Ovesna, Petra and Bryja, Vítězslav and Souček, Karel},
   article_location = {BERLIN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-024-58236-y},
   keywords = {In vivo model; Melanoma; Circulating tumor cells; Metastasis; Tumorectomy},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Orthotopic model for the analysis of melanoma circulating tumor cells},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2486358
AU  - Picková, Markéta - Kahounová, Zuzana - Radaszkiewicz, Tomasz Witold - Procházková, Jiřina - Fedr, Radek - Noskova, Michaela - Radaszkiewicz, Katarzyna Anna - Ovesna, Petra - Bryja, Vítězslav - Souček, Karel
PY  - 2024
TI  - Orthotopic model for the analysis of melanoma circulating tumor cells
JF  - Nature Scientific Reports
VL  - 14
IS  - 1
PB  - NATURE PORTFOLIO
SN  - 20452322
KW  - In vivo model
KW  - Melanoma
KW  - Circulating tumor cells
KW  - Metastasis
KW  - Tumorectomy
N2  - Metastatic melanoma, a highly lethal form of skin cancer, presents significant clinical challenges due to limited therapeutic options and high metastatic capacity. Recent studies have demonstrated that cancer dissemination can occur earlier, before the diagnosis of the primary tumor. The progress in understanding the kinetics of cancer dissemination is limited by the lack of animal models that accurately mimic disease progression. We have established a xenograft model of human melanoma that spontaneously metastasizes to lymph nodes and lungs. This model allows precise monitoring of melanoma progression and is suitable for the quantitative and qualitative analysis of circulating tumor cells (CTCs). We have validated a flow cytometry-based protocol for CTCs enumeration and isolation. We could demonstrate that (i) CTCs were detectable in the bloodstream from the fourth week after tumor initiation, coinciding with the lymph node metastases appearance, (ii) excision of the primary tumor accelerated the formation of metastases in lymph nodes and lungs as early as one-week post-surgery, accompanied by the increased numbers of CTCs, and (iii) CTCs change their surface protein signature. In summary, we present a model of human melanoma that can be effectively utilized for future drug efficacy studies.
ER  -

PICKOVÁ, Markéta, Zuzana KAHOUNOVÁ, Tomasz Witold RADASZKIEWICZ, Jiřina PROCHÁZKOVÁ, Radek FEDR, Michaela NOSKOVA, Katarzyna Anna RADASZKIEWICZ, Petra OVESNA, Vítězslav BRYJA a Karel SOUČEK. Orthotopic model for the analysis of melanoma circulating tumor cells. \textit{Nature Scientific Reports}. BERLIN: NATURE PORTFOLIO, 2024, roč.~14, č.~1, 13 s. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-024-58236-y.

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