Evaluating stress resilience of cyanobacteria through flow
cytometry and fluorescent viability assessment

J 2025

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

KROUPOVA, Zuzana; Eva SLANINOVA; Katerina MRAZOVA; Vladislav KRZYZANEK; Kamila HRUBANOVA et. al.

Základní údaje

Originální název

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Autoři

KROUPOVA, Zuzana; Eva SLANINOVA; Katerina MRAZOVA; Vladislav KRZYZANEK; Kamila HRUBANOVA; Ines FRITZ a Stanislav OBRUCA

Vydání

Folia microbiologica, Praha, Institute of Microbiology, Academy of Sciences of the Czech Republic, 2025, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.100 v roce 2024

Organizační jednotka

CIISB III

DOI

https://doi.org/10.1007/s12223-024-01212-w

UT WoS

001349486800001

EID Scopus

2-s2.0-85208216503

Klíčová slova anglicky

Biotechnology; Cyanobacteria; Flow cytometry; Fluorescent viability probes; Stress resistance; Viability assessment

Štítky

CF CRYO, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2025 10:13, Mgr. Eva Dubská

Anotace

V originále

Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.

Návaznosti

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

KROUPOVA, Zuzana; Eva SLANINOVA; Katerina MRAZOVA; Vladislav KRZYZANEK; Kamila HRUBANOVA; Ines FRITZ a Stanislav OBRUCA. Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment. Folia microbiologica. Praha: Institute of Microbiology, Academy of Sciences of the Czech Republic, 2025, roč. 70, č. 1, s. 205-223. ISSN 0015-5632. Dostupné z: https://doi.org/10.1007/s12223-024-01212-w.

@article{2486563,
   author = {Kroupova, Zuzana and Slaninova, Eva and Mrazova, Katerina and Krzyzanek, Vladislav and Hrubanova, Kamila and Fritz, Ines and Obruca, Stanislav},
   article_location = {Praha},
   article_number = {1},
   doi = {https://doi.org/10.1007/s12223-024-01212-w},
   keywords = {Biotechnology; Cyanobacteria; Flow cytometry; Fluorescent viability probes; Stress resistance; Viability assessment},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia microbiologica},
   title = {Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment},
   url = {https://link.springer.com/article/10.1007/s12223-024-01212-w},
   volume = {70},
   year = {2025}
}

TY  - JOUR
ID  - 2486563
AU  - Kroupova, Zuzana - Slaninova, Eva - Mrazova, Katerina - Krzyzanek, Vladislav - Hrubanova, Kamila - Fritz, Ines - Obruca, Stanislav
PY  - 2025
TI  - Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment
JF  - Folia microbiologica
VL  - 70
IS  - 1
SP  - 205-223
EP  - 205-223
PB  - Institute of Microbiology, Academy of Sciences of the Czech Republic
SN  - 00155632
KW  - Biotechnology
KW  - Cyanobacteria
KW  - Flow cytometry
KW  - Fluorescent viability probes
KW  - Stress resistance
KW  - Viability assessment
UR  - https://link.springer.com/article/10.1007/s12223-024-01212-w
N2  - Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.
ER  -

KROUPOVA, Zuzana; Eva SLANINOVA; Katerina MRAZOVA; Vladislav KRZYZANEK; Kamila HRUBANOVA; Ines FRITZ a Stanislav OBRUCA. Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment. \textit{Folia microbiologica}. Praha: Institute of Microbiology, Academy of Sciences of the Czech Republic, 2025, roč.~70, č.~1, s.~205-223. ISSN~0015-5632. Dostupné z: https://doi.org/10.1007/s12223-024-01212-w.

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