Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
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WELSH, Joshua A; Deborah C I GOBERDHAN; Lorraine O'DRISCOLL; Edit I BUZAS; Cherie BLENKIRON; Benedetta BUSSOLATI; Houjian CAI; Di Vizio DOLORES; Tom A P DRIEDONKS; Uta ERDBRUGGER; Juan M FALCON-PEREZ; Qing-Ling FU; Andrew F HILL; Metka LENASSI; Sai Kiang LIM; My G MAHONEY; Sujata MOHANTY; Andreas MOLLER; Rienk NIEUWLAND; Takahiro OCHIYA; Susmita SAHOO; Ana C TORRECILHAS; Lei ZHENG; Andries ZIJLSTRA; Sarah ABUELREICH; Reem BAGABAS; Paolo BERGESE; Esther M BRIDGES; Marco BRUCALE; Dylan BURGER; Randy P CARNEY; Emanuele COCUCCI; Federico COLOMBO; Rossella CRESCITELLI; Edveena HANSER; Adrian L HARRIS; Norman J HAUGHEY; An HENDRIX; Alexander R IVANOV; Tijana JOVANOVIC-TALISMAN; Nicole A KRUH-GARCIA; Vroniqa Ku'ulei-Lyn FAUSTINO; Diego KYBURZ; Cecilia LASSER; Kathleen M LENNON; Jan LOTVALL; Adam L MADDOX; Elena S MARTENS-UZUNOVA; Rachel R MIZENKO; Lauren A NEWMAN; Andrea RIDOLFI; Eva ROHDE; Tatu ROJALIN; Andrew ROWLAND; Andras SAFTICS; Ursula S SANDAU; Julie A SAUGSTAD; Faezeh SHEKARI; Simon SWIFT; Dmitry TER-OVANESYAN; Juan P TOSAR; Zivile USECKAITE; Francesco VALLE; Zoltan VARGA; van der Pol EDWIN; van Herwijnen Martijn J C; Marca H M WAUBEN; Ann M WEHMAN; Sarah WILLIAMS; Andrea ZENDRINI; Alan J ZIMMERMAN; Clotilde THERY; Kenneth W WITWER a Vendula HLAVÁČKOVÁ POSPÍCHALOVÁ
Vydání
Journal of Extracellular Vesicles, Wiley, 2024, 2001-3078
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
WELSH, Joshua A; Deborah C I GOBERDHAN; Lorraine O'DRISCOLL; Edit I BUZAS; Cherie BLENKIRON; Benedetta BUSSOLATI; Houjian CAI; Di Vizio DOLORES; Tom A P DRIEDONKS; Uta ERDBRUGGER; Juan M FALCON-PEREZ; Qing-Ling FU; Andrew F HILL; Metka LENASSI; Sai Kiang LIM; My G MAHONEY; Sujata MOHANTY; Andreas MOLLER; Rienk NIEUWLAND; Takahiro OCHIYA; Susmita SAHOO; Ana C TORRECILHAS; Lei ZHENG; Andries ZIJLSTRA; Sarah ABUELREICH; Reem BAGABAS; Paolo BERGESE; Esther M BRIDGES; Marco BRUCALE; Dylan BURGER; Randy P CARNEY; Emanuele COCUCCI; Federico COLOMBO; Rossella CRESCITELLI; Edveena HANSER; Adrian L HARRIS; Norman J HAUGHEY; An HENDRIX; Alexander R IVANOV; Tijana JOVANOVIC-TALISMAN; Nicole A KRUH-GARCIA; Vroniqa Ku'ulei-Lyn FAUSTINO; Diego KYBURZ; Cecilia LASSER; Kathleen M LENNON; Jan LOTVALL; Adam L MADDOX; Elena S MARTENS-UZUNOVA; Rachel R MIZENKO; Lauren A NEWMAN; Andrea RIDOLFI; Eva ROHDE; Tatu ROJALIN; Andrew ROWLAND; Andras SAFTICS; Ursula S SANDAU; Julie A SAUGSTAD; Faezeh SHEKARI; Simon SWIFT; Dmitry TER-OVANESYAN; Juan P TOSAR; Zivile USECKAITE; Francesco VALLE; Zoltan VARGA; van der Pol EDWIN; van Herwijnen Martijn J C; Marca H M WAUBEN; Ann M WEHMAN; Sarah WILLIAMS; Andrea ZENDRINI; Alan J ZIMMERMAN; Clotilde THERY; Kenneth W WITWER a Vendula HLAVÁČKOVÁ POSPÍCHALOVÁ. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles. Wiley, 2024, roč. 13, č. 2, s. e12404, 84 s. ISSN 2001-3078. Dostupné z: https://doi.org/10.1002/jev2.12404.
@article{2486637, author = {Welsh, Joshua A and Goberdhan, Deborah C I and O'Driscoll, Lorraine and Buzas, Edit I and Blenkiron, Cherie and Bussolati, Benedetta and Cai, Houjian and Dolores, Di Vizio and Driedonks, Tom A P and Erdbrugger, Uta and FalconandPerez, Juan M and Fu, QingandLing and Hill, Andrew F and Lenassi, Metka and Lim, Sai Kiang and Mahoney, My G and Mohanty, Sujata and Moller, Andreas and Nieuwland, Rienk and Ochiya, Takahiro and Sahoo, Susmita and Torrecilhas, Ana C and Zheng, Lei and Zijlstra, Andries and Abuelreich, Sarah and Bagabas, Reem and Bergese, Paolo and Bridges, Esther M and Brucale, Marco and Burger, Dylan and Carney, Randy P and Cocucci, Emanuele and Colombo, Federico and Crescitelli, Rossella and Hanser, Edveena and Harris, Adrian L and Haughey, Norman J and Hendrix, An and Ivanov, Alexander R and JovanovicandTalisman, Tijana and KruhandGarcia, Nicole A and Faustino, Vroniqa Ku'uleiandLyn and Kyburz, Diego and Lasser, Cecilia and Lennon, Kathleen M and Lotvall, Jan and Maddox, Adam L and MartensandUzunova, Elena S and Mizenko, Rachel R and Newman, Lauren A and Ridolfi, Andrea and Rohde, Eva and Rojalin, Tatu and Rowland, Andrew and Saftics, Andras and Sandau, Ursula S and Saugstad, Julie A and Shekari, Faezeh and Swift, Simon and TerandOvanesyan, Dmitry and Tosar, Juan P and Useckaite, Zivile and Valle, Francesco and Varga, Zoltan and Edwin, van der Pol and C, van Herwijnen Martijn J and Wauben, Marca H M and Wehman, Ann M and Williams, Sarah and Zendrini, Andrea and Zimmerman, Alan J and Thery, Clotilde and Witwer, Kenneth W and Hlaváčková Pospíchalová, Vendula}, article_number = {2}, doi = {https://doi.org/10.1002/jev2.12404}, keywords = {ectosomes; exosomes; extracellular vesicles; extracellular particles; guidelines; microparticles; microvesicles; minimal information requirements; MISEV; reproducibility; rigor; standardisation}, language = {eng}, issn = {2001-3078}, journal = {Journal of Extracellular Vesicles}, title = {Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches}, url = {https://doi.org/10.1002/jev2.12404}, volume = {13}, year = {2024} }
TY - JOUR ID - 2486637 AU - Welsh, Joshua A - Goberdhan, Deborah C I - O'Driscoll, Lorraine - Buzas, Edit I - Blenkiron, Cherie - Bussolati, Benedetta - Cai, Houjian - Dolores, Di Vizio - Driedonks, Tom A P - Erdbrugger, Uta - Falcon-Perez, Juan M - Fu, Qing-Ling - Hill, Andrew F - Lenassi, Metka - Lim, Sai Kiang - Mahoney, My G - Mohanty, Sujata - Moller, Andreas - Nieuwland, Rienk - Ochiya, Takahiro - Sahoo, Susmita - Torrecilhas, Ana C - Zheng, Lei - Zijlstra, Andries - Abuelreich, Sarah - Bagabas, Reem - Bergese, Paolo - Bridges, Esther M - Brucale, Marco - Burger, Dylan - Carney, Randy P - Cocucci, Emanuele - Colombo, Federico - Crescitelli, Rossella - Hanser, Edveena - Harris, Adrian L - Haughey, Norman J - Hendrix, An - Ivanov, Alexander R - Jovanovic-Talisman, Tijana - Kruh-Garcia, Nicole A - Faustino, Vroniqa Ku'ulei-Lyn - Kyburz, Diego - Lasser, Cecilia - Lennon, Kathleen M - Lotvall, Jan - Maddox, Adam L - Martens-Uzunova, Elena S - Mizenko, Rachel R - Newman, Lauren A - Ridolfi, Andrea - Rohde, Eva - Rojalin, Tatu - Rowland, Andrew - Saftics, Andras - Sandau, Ursula S - Saugstad, Julie A - Shekari, Faezeh - Swift, Simon - Ter-Ovanesyan, Dmitry - Tosar, Juan P - Useckaite, Zivile - Valle, Francesco - Varga, Zoltan - Edwin, van der Pol - C, van Herwijnen Martijn J - Wauben, Marca H M - Wehman, Ann M - Williams, Sarah - Zendrini, Andrea - Zimmerman, Alan J - Thery, Clotilde - Witwer, Kenneth W - Hlaváčková Pospíchalová, Vendula PY - 2024 TI - Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches JF - Journal of Extracellular Vesicles VL - 13 IS - 2 SP - e12404 EP - e12404 PB - Wiley SN - 20013078 KW - ectosomes KW - exosomes KW - extracellular vesicles KW - extracellular particles KW - guidelines KW - microparticles KW - microvesicles KW - minimal information requirements KW - MISEV KW - reproducibility KW - rigor KW - standardisation UR - https://doi.org/10.1002/jev2.12404 N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly. ER -
WELSH, Joshua A; Deborah C I GOBERDHAN; Lorraine O'DRISCOLL; Edit I BUZAS; Cherie BLENKIRON; Benedetta BUSSOLATI; Houjian CAI; Di Vizio DOLORES; Tom A P DRIEDONKS; Uta ERDBRUGGER; Juan M FALCON-PEREZ; Qing-Ling FU; Andrew F HILL; Metka LENASSI; Sai Kiang LIM; My G MAHONEY; Sujata MOHANTY; Andreas MOLLER; Rienk NIEUWLAND; Takahiro OCHIYA; Susmita SAHOO; Ana C TORRECILHAS; Lei ZHENG; Andries ZIJLSTRA; Sarah ABUELREICH; Reem BAGABAS; Paolo BERGESE; Esther M BRIDGES; Marco BRUCALE; Dylan BURGER; Randy P CARNEY; Emanuele COCUCCI; Federico COLOMBO; Rossella CRESCITELLI; Edveena HANSER; Adrian L HARRIS; Norman J HAUGHEY; An HENDRIX; Alexander R IVANOV; Tijana JOVANOVIC-TALISMAN; Nicole A KRUH-GARCIA; Vroniqa Ku'ulei-Lyn FAUSTINO; Diego KYBURZ; Cecilia LASSER; Kathleen M LENNON; Jan LOTVALL; Adam L MADDOX; Elena S MARTENS-UZUNOVA; Rachel R MIZENKO; Lauren A NEWMAN; Andrea RIDOLFI; Eva ROHDE; Tatu ROJALIN; Andrew ROWLAND; Andras SAFTICS; Ursula S SANDAU; Julie A SAUGSTAD; Faezeh SHEKARI; Simon SWIFT; Dmitry TER-OVANESYAN; Juan P TOSAR; Zivile USECKAITE; Francesco VALLE; Zoltan VARGA; van der Pol EDWIN; van Herwijnen Martijn J C; Marca H M WAUBEN; Ann M WEHMAN; Sarah WILLIAMS; Andrea ZENDRINI; Alan J ZIMMERMAN; Clotilde THERY; Kenneth W WITWER a Vendula HLAVÁČKOVÁ POSPÍCHALOVÁ. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. \textit{Journal of Extracellular Vesicles}. Wiley, 2024, roč.~13, č.~2, s.~e12404, 84 s. ISSN~2001-3078. Dostupné z: https://doi.org/10.1002/jev2.12404.