Structural Characterization of Monoclonal Antibodies and
Epitope Mapping by FFAP Footprinting

J 2024

Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

FOJTIK, Lukas; Zuzana KALANINOVA; Jan FIALA; Petr HALADA; Josef CHMELIK et al.

Základní údaje

Originální název

Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

Autoři

FOJTIK, Lukas; Zuzana KALANINOVA; Jan FIALA; Petr HALADA; Josef CHMELIK; Petr MAN; Zdenek KUKACKA a Petr NOVAK

Vydání

Analytical chemistry, WASHINGTON, AMER CHEMICAL SOC, 2024, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90127/24:00139172

Organizační jednotka

CIISB II

Klíčová slova anglicky

MASS; EXCHANGE; IDENTIFICATION; COMPLEX; PROTEINS; SEQUENCE

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 3. 2025 18:19, Mgr. Eva Dubská

Anotace

V originále

Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

FOJTIK, Lukas; Zuzana KALANINOVA; Jan FIALA; Petr HALADA; Josef CHMELIK; Petr MAN; Zdenek KUKACKA a Petr NOVAK. Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting. Analytical chemistry. WASHINGTON: AMER CHEMICAL SOC, 2024, roč. 96, č. 19, s. 7386-7393. ISSN 0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.3c04161.

@article{2486880,
   author = {Fojtik, Lukas and Kalaninova, Zuzana and Fiala, Jan and Halada, Petr and Chmelik, Josef and Man, Petr and Kukacka, Zdenek and Novak, Petr},
   article_location = {WASHINGTON},
   article_number = {19},
   doi = {https://doi.org/10.1021/acs.analchem.3c04161},
   keywords = {MASS; EXCHANGE; IDENTIFICATION; COMPLEX; PROTEINS; SEQUENCE},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161},
   volume = {96},
   year = {2024}
}

TY  - JOUR
ID  - 2486880
AU  - Fojtik, Lukas - Kalaninova, Zuzana - Fiala, Jan - Halada, Petr - Chmelik, Josef - Man, Petr - Kukacka, Zdenek - Novak, Petr
PY  - 2024
TI  - Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting
JF  - Analytical chemistry
VL  - 96
IS  - 19
SP  - 7386-7393
EP  - 7386-7393
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - MASS
KW  - EXCHANGE
KW  - IDENTIFICATION
KW  - COMPLEX
KW  - PROTEINS
KW  - SEQUENCE
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161
N2  - Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.
ER  -

FOJTIK, Lukas; Zuzana KALANINOVA; Jan FIALA; Petr HALADA; Josef CHMELIK; Petr MAN; Zdenek KUKACKA a Petr NOVAK. Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting. \textit{Analytical chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2024, roč.~96, č.~19, s.~7386-7393. ISSN~0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.3c04161.

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