Combined in vitro and cell-based selection display
method producing specific binders against IL-9 receptor in high
yields

J 2023

Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields

HULICIAK, Maros; Lada BIEDERMANOVA; Daniel BERDAR; Stepan HERYNEK; Lucie KOLAROVA et al.

Základní údaje

Originální název

Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields

Autoři

HULICIAK, Maros; Lada BIEDERMANOVA; Daniel BERDAR; Stepan HERYNEK; Lucie KOLAROVA; Jakub TOMALA; Pavel MIKULECKY a Bohdan SCHNEIDER

Vydání

FEBS Journal, MALDEN, Blackwell, 2023, 1742-464X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.500

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90127/23:00139188

Organizační jednotka

CIISB II

Klíčová slova anglicky

directed evolution; interleukin 9 receptor alpha; protein scaffolds; ribosome display; yeast display

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2025 09:18, Mgr. Eva Dubská

Anotace

V originále

We combined cell-free ribosome display and cell-based yeast display selection to build specific protein binders to the extracellular domain of the human interleukin 9 receptor alpha (IL-9R alpha). The target, IL-9R alpha, is the receptor involved in the signalling pathway of IL-9, a pro-inflammatory cytokine medically important for its involvement in respiratory diseases. The successive use of modified protocols of ribosome and yeast displays allowed us to combine their strengths-the virtually infinite selection power of ribosome display and the production of (mostly) properly folded and soluble proteins in yeast display. The described experimental protocol is optimized to produce binders highly specific to the target, including selectivity to common proteins such as BSA, and proteins potentially competing for the binder such as receptors of other cytokines. The binders were trained from DNA libraries of two protein scaffolds called 57aBi and 57bBi developed in our laboratory. We show that the described unconventional combination of ribosome and yeast displays is effective in developing selective small protein binders to the medically relevant molecular target.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

HULICIAK, Maros; Lada BIEDERMANOVA; Daniel BERDAR; Stepan HERYNEK; Lucie KOLAROVA; Jakub TOMALA; Pavel MIKULECKY a Bohdan SCHNEIDER. Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields. FEBS Journal. MALDEN: Blackwell, 2023, roč. 290, č. 11, s. 2993-3005. ISSN 1742-464X. Dostupné z: https://doi.org/10.1111/febs.16726.

@article{2487182,
 author = {Huliciak, Maros and Biedermanova, Lada and Berdar, Daniel and Herynek, Stepan and Kolarova, Lucie and Tomala, Jakub and Mikulecky, Pavel and Schneider, Bohdan},
 article_location = {MALDEN},
 article_number = {11},
 doi = {https://doi.org/10.1111/febs.16726},
 keywords = {directed evolution; interleukin 9 receptor alpha; protein scaffolds; ribosome display; yeast display},
 language = {eng},
 issn = {1742-464X},
 journal = {FEBS Journal},
 title = {Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields},
 url = {https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16726},
 volume = {290},
 year = {2023}
}

TY - JOUR
ID - 2487182
AU - Huliciak, Maros - Biedermanova, Lada - Berdar, Daniel - Herynek, Stepan - Kolarova, Lucie - Tomala, Jakub - Mikulecky, Pavel - Schneider, Bohdan
PY - 2023
TI - Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields
JF - FEBS Journal
VL - 290
IS - 11
SP - 2993-3005
EP - 2993-3005
PB - Blackwell
SN - 1742464X
KW - directed evolution
KW - interleukin 9 receptor alpha
KW - protein scaffolds
KW - ribosome display
KW - yeast display
UR - https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16726
N2 - We combined cell-free ribosome display and cell-based yeast display selection to build specific protein binders to the extracellular domain of the human interleukin 9 receptor alpha (IL-9R alpha). The target, IL-9R alpha, is the receptor involved in the signalling pathway of IL-9, a pro-inflammatory cytokine medically important for its involvement in respiratory diseases. The successive use of modified protocols of ribosome and yeast displays allowed us to combine their strengths-the virtually infinite selection power of ribosome display and the production of (mostly) properly folded and soluble proteins in yeast display. The described experimental protocol is optimized to produce binders highly specific to the target, including selectivity to common proteins such as BSA, and proteins potentially competing for the binder such as receptors of other cytokines. The binders were trained from DNA libraries of two protein scaffolds called 57aBi and 57bBi developed in our laboratory. We show that the described unconventional combination of ribosome and yeast displays is effective in developing selective small protein binders to the medically relevant molecular target.
ER -

HULICIAK, Maros; Lada BIEDERMANOVA; Daniel BERDAR; Stepan HERYNEK; Lucie KOLAROVA; Jakub TOMALA; Pavel MIKULECKY a Bohdan SCHNEIDER. Combined \&{}lt;i\&{}gt;in vitro\&{}lt;/i\&{}gt; and cell-based selection display method producing specific binders against IL-9 receptor in high yields. \textit{FEBS Journal}. MALDEN: Blackwell, 2023, roč.~290, č.~11, s.~2993-3005. ISSN~1742-464X. Dostupné z: https://doi.org/10.1111/febs.16726.

Přehled o publikaci

Combined <i>in vitro</i> and cell-based selection display method producing specific binders against IL-9 receptor in high yields