Diffraction anisotropy and paired refinement: crystal structure
of H33, a protein binder to interleukin 10

J 2023

Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10

KOLENKO, Petr; Pavel MIKULECKY; Phuong Ngoc PHAM; Martin MALY; Bohdan SCHNEIDER et al.

Základní údaje

Originální název

Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10

Autoři

KOLENKO, Petr; Pavel MIKULECKY; Phuong Ngoc PHAM; Martin MALY a Bohdan SCHNEIDER

Vydání

Journal of Applied Crystallography, Chester, INT UNION CRYSTALLOGRAPHY, 2023, 1600-5767

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90127/23:00139201

Organizační jednotka

CIISB II

Klíčová slova anglicky

anisotropy; paired refinement; binder H33

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2025 15:24, Mgr. Eva Dubská

Anotace

V originále

Binder H33 is a small protein binder engineered by ribosome display to bind human interleukin 10. Crystals of binder H33 display severe diffraction anisotropy. A set of data files with correction for diffraction anisotropy based on different local signal-to-noise ratios was prepared. Paired refinement was used to find the optimal anisotropic high-resolution diffraction limit of the data: 3.13-2.47 angstrom. The structure of binder H33 belongs to the 2% of crystal structures with the highest solvent content in the Protein Data Bank.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KOLENKO, Petr; Pavel MIKULECKY; Phuong Ngoc PHAM; Martin MALY a Bohdan SCHNEIDER. Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10. Journal of Applied Crystallography. Chester: INT UNION CRYSTALLOGRAPHY, 2023, roč. 56, č. 4, s. 1261-1266. ISSN 1600-5767. Dostupné z: https://doi.org/10.1107/S160057672300479X.

@article{2487282,
   author = {Kolenko, Petr and Mikulecky, Pavel and Pham, Phuong Ngoc and Maly, Martin and Schneider, Bohdan},
   article_location = {Chester},
   article_number = {4},
   doi = {https://doi.org/10.1107/S160057672300479X},
   keywords = {anisotropy; paired refinement; binder H33},
   language = {eng},
   issn = {1600-5767},
   journal = {Journal of Applied Crystallography},
   title = {Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10},
   url = {https://journals.iucr.org/j/issues/2023/04/00/ap5049/index.html},
   volume = {56},
   year = {2023}
}

TY  - JOUR
ID  - 2487282
AU  - Kolenko, Petr - Mikulecky, Pavel - Pham, Phuong Ngoc - Maly, Martin - Schneider, Bohdan
PY  - 2023
TI  - Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10
JF  - Journal of Applied Crystallography
VL  - 56
IS  - 4
SP  - 1261-1266
EP  - 1261-1266
PB  - INT UNION CRYSTALLOGRAPHY
SN  - 16005767
KW  - anisotropy
KW  - paired refinement
KW  - binder H33
UR  - https://journals.iucr.org/j/issues/2023/04/00/ap5049/index.html
N2  - Binder H33 is a small protein binder engineered by ribosome display to bind human interleukin 10. Crystals of binder H33 display severe diffraction anisotropy. A set of data files with correction for diffraction anisotropy based on different local signal-to-noise ratios was prepared. Paired refinement was used to find the optimal anisotropic high-resolution diffraction limit of the data: 3.13-2.47 angstrom. The structure of binder H33 belongs to the 2% of crystal structures with the highest solvent content in the Protein Data Bank.
ER  -

KOLENKO, Petr; Pavel MIKULECKY; Phuong Ngoc PHAM; Martin MALY a Bohdan SCHNEIDER. Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10. \textit{Journal of Applied Crystallography}. Chester: INT UNION CRYSTALLOGRAPHY, 2023, roč.~56, č.~4, s.~1261-1266. ISSN~1600-5767. Dostupné z: https://doi.org/10.1107/S160057672300479X.

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