Motif orientation matters: Structural characterization of TEAD1
recognition of genomic DNA

J 2021

Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA

FILANDROVA, Ruzena; Karel VALIS; Jiri CERMY; Josef CHMELIK; Lukas SLAVATA et al.

Základní údaje

Originální název

Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA

Autoři

FILANDROVA, Ruzena; Karel VALIS; Jiri CERMY; Josef CHMELIK; Lukas SLAVATA; Jan FIALA; Michal ROSULEK; Daniel KAVAN; Petr MAN; Tomas CHUM; Marek CEBECAUER; Daniele FABRIS a Petr NOVAK

Vydání

Structure, CAMBRIDGE, CELL PRESS, 2021, 0969-2126

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.871

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90043/21:00139216

Organizační jednotka

CIISB

Klíčová slova anglicky

CHEMICAL CROSS-LINKING; TRANSCRIPTION FACTORS; HIPPO PATHWAY; BINDING-SITES; WEB SERVER; PROTEIN; YAP; EXPRESSION; FAMILY; DOMAIN

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2025 20:30, Mgr. Eva Dubská

Anotace

V originále

TEAD transcription factors regulate gene expression through interactions with DNA and other proteins. They are crucial for the development of eukaryotic organisms and to control the expression of genes involved mostly in cell proliferation and differentiation; however, their deregulation can lead to tumorigenesis. To study the interactions of TEAD1 with M-CAT motifs and their inverted versions, the K-D of each complex was determined, and H/D exchange, quantitative chemical cross-linking, molecular docking, and smFRET were utilized for structural characterization. ChIP-qPCR was employed to correlate the results with a cell line model. The results obtained showed that although the inverted motif has 103higher K-D, the same residues were affected by the presence of M-CAT in both orientations. Molecular docking and smFRET revealed that TEAD1 binds the inverted motif rotated 180 degrees. In addition, the inverted motif was proven to be occupied by TEAD1 in Jurkat cells, suggesting that the low-affinity binding sites present in the human genome may possess biological relevance.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

FILANDROVA, Ruzena; Karel VALIS; Jiri CERMY; Josef CHMELIK; Lukas SLAVATA; Jan FIALA; Michal ROSULEK; Daniel KAVAN; Petr MAN; Tomas CHUM; Marek CEBECAUER; Daniele FABRIS a Petr NOVAK. Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA. Structure. CAMBRIDGE: CELL PRESS, 2021, roč. 29, č. 4, s. 345-364. ISSN 0969-2126. Dostupné z: https://doi.org/10.1016/j.str.2020.11.018.

@article{2487326,
   author = {Filandrova, Ruzena and Valis, Karel and Cermy, Jiri and Chmelik, Josef and Slavata, Lukas and Fiala, Jan and Rosulek, Michal and Kavan, Daniel and Man, Petr and Chum, Tomas and Cebecauer, Marek and Fabris, Daniele and Novak, Petr},
   article_location = {CAMBRIDGE},
   article_number = {4},
   doi = {https://doi.org/10.1016/j.str.2020.11.018},
   keywords = {CHEMICAL CROSS-LINKING; TRANSCRIPTION FACTORS; HIPPO PATHWAY; BINDING-SITES; WEB SERVER; PROTEIN; YAP; EXPRESSION; FAMILY; DOMAIN},
   language = {eng},
   issn = {0969-2126},
   journal = {Structure},
   title = {Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA},
   url = {https://www.sciencedirect.com/science/article/pii/S0969212620304652?via%3Dihub},
   volume = {29},
   year = {2021}
}

TY  - JOUR
ID  - 2487326
AU  - Filandrova, Ruzena - Valis, Karel - Cermy, Jiri - Chmelik, Josef - Slavata, Lukas - Fiala, Jan - Rosulek, Michal - Kavan, Daniel - Man, Petr - Chum, Tomas - Cebecauer, Marek - Fabris, Daniele - Novak, Petr
PY  - 2021
TI  - Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA
JF  - Structure
VL  - 29
IS  - 4
SP  - 345-364
EP  - 345-364
PB  - CELL PRESS
SN  - 09692126
KW  - CHEMICAL CROSS-LINKING
KW  - TRANSCRIPTION FACTORS
KW  - HIPPO PATHWAY
KW  - BINDING-SITES
KW  - WEB SERVER
KW  - PROTEIN
KW  - YAP
KW  - EXPRESSION
KW  - FAMILY
KW  - DOMAIN
UR  - https://www.sciencedirect.com/science/article/pii/S0969212620304652?via%3Dihub
N2  - TEAD transcription factors regulate gene expression through interactions with DNA and other proteins. They are crucial for the development of eukaryotic organisms and to control the expression of genes involved mostly in cell proliferation and differentiation; however, their deregulation can lead to tumorigenesis. To study the interactions of TEAD1 with M-CAT motifs and their inverted versions, the K-D of each complex was determined, and H/D exchange, quantitative chemical cross-linking, molecular docking, and smFRET were utilized for structural characterization. ChIP-qPCR was employed to correlate the results with a cell line model. The results obtained showed that although the inverted motif has 103higher K-D, the same residues were affected by the presence of M-CAT in both orientations. Molecular docking and smFRET revealed that TEAD1 binds the inverted motif rotated 180 degrees. In addition, the inverted motif was proven to be occupied by TEAD1 in Jurkat cells, suggesting that the low-affinity binding sites present in the human genome may possess biological relevance.
ER  -

FILANDROVA, Ruzena; Karel VALIS; Jiri CERMY; Josef CHMELIK; Lukas SLAVATA; Jan FIALA; Michal ROSULEK; Daniel KAVAN; Petr MAN; Tomas CHUM; Marek CEBECAUER; Daniele FABRIS a Petr NOVAK. Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA. \textit{Structure}. CAMBRIDGE: CELL PRESS, 2021, roč.~29, č.~4, s.~345-364. ISSN~0969-2126. Dostupné z: https://doi.org/10.1016/j.str.2020.11.018.

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