Femtosecond-to-nanosecond dynamics of flavin mononucleotide
monitored by stimulated Raman spectroscopy and simulations

J 2020

Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations

ANDRIKOPOULOS, Prokopis C; Yingliang LIU; Alessandra PICCHIOTTI; Nils LENNGREN; Miroslav KLOZ et al.

Základní údaje

Originální název

Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations

Autoři

Vydání

Physical Chemistry Chemical Physics, CAMBRIDGE, Royal Society of Chemistry, 2020, 1463-9076

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.676

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90043/20:00139218

Organizační jednotka

CIISB

Klíčová slova anglicky

ADENINE-DINUCLEOTIDE FADEXCITED-STATE; ELECTRON-TRANSFER; LOV2 DOMAIN; ABSORPTION-SPECTRA; COFACTOR FLAVIN; BASIS-SETS; RIBOFLAVIN; PHOTOTROPINFMN

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2025 20:38, Mgr. Eva Dubská

Anotace

V originále

Flavin mononucleotide (FMN) belongs to the large family of flavins, ubiquitous yellow-coloured biological chromophores that contain an isoalloxazine ring system. As a cofactor in flavoproteins, it is found in various enzymes and photosensory receptors, like those featuring the light-oxygen-voltage (LOV) domain. The photocycle of FMN is triggered by blue light and proceeds via a cascade of intermediate states. In this work, we have studied isolated FMN in an aqueous solution in order to elucidate the intrinsic electronic and vibrational changes of the chromophore upon excitation. The ultrafast transitions of excited FMN were monitored through the joint use of femtosecond stimulated Raman spectroscopy (FSRS) and transient absorption spectroscopy encompassing a time window between 0 ps and 6 ns with 50 fs time resolution. Global analysis of the obtained transient visible absorption and transient Raman spectra in combination with extensive quantum chemistry calculations identified unambiguously the singlet and triplet FMN populations and addressed solvent dynamics effects. The good agreement between the experimental and theoretical spectra facilitated the assignment of electronic transitions and vibrations. Our results represent the first steps towards more complex experiments aimed at tracking structural changes of FMN embedded in light-inducible proteins upon photoexcitation.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

ANDRIKOPOULOS, Prokopis C; Yingliang LIU; Alessandra PICCHIOTTI; Nils LENNGREN; Miroslav KLOZ; Aditya S CHAUDHARI; Martin PRECEK; Mateusz REBARZ; Jakob ANDREASSON; Janos HAJDU; Bohdan SCHNEIDER a Gustavo FUERTES. Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations. Physical Chemistry Chemical Physics. CAMBRIDGE: Royal Society of Chemistry, 2020, roč. 22, č. 12, s. 6538-6552. ISSN 1463-9076. Dostupné z: https://doi.org/10.1039/c9cp04918e.

@article{2487330,
   author = {Andrikopoulos, Prokopis C and Liu, Yingliang and Picchiotti, Alessandra and Lenngren, Nils and Kloz, Miroslav and Chaudhari, Aditya S and Precek, Martin and Rebarz, Mateusz and Andreasson, Jakob and Hajdu, Janos and Schneider, Bohdan and Fuertes, Gustavo},
   article_location = {CAMBRIDGE},
   article_number = {12},
   doi = {https://doi.org/10.1039/c9cp04918e},
   keywords = {ADENINE-DINUCLEOTIDE FADEXCITED-STATE; ELECTRON-TRANSFER; LOV2 DOMAIN; ABSORPTION-SPECTRA; COFACTOR FLAVIN; BASIS-SETS; RIBOFLAVIN; PHOTOTROPINFMN},
   language = {eng},
   issn = {1463-9076},
   journal = {Physical Chemistry Chemical Physics},
   title = {Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations},
   url = {https://pubs.rsc.org/en/content/articlelanding/2020/cp/c9cp04918e},
   volume = {22},
   year = {2020}
}

TY  - JOUR
ID  - 2487330
AU  - Andrikopoulos, Prokopis C - Liu, Yingliang - Picchiotti, Alessandra - Lenngren, Nils - Kloz, Miroslav - Chaudhari, Aditya S - Precek, Martin - Rebarz, Mateusz - Andreasson, Jakob - Hajdu, Janos - Schneider, Bohdan - Fuertes, Gustavo
PY  - 2020
TI  - Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations
JF  - Physical Chemistry Chemical Physics
VL  - 22
IS  - 12
SP  - 6538-6552
EP  - 6538-6552
PB  - Royal Society of Chemistry
SN  - 14639076
KW  - ADENINE-DINUCLEOTIDE FADEXCITED-STATE
KW  - ELECTRON-TRANSFER
KW  - LOV2 DOMAIN
KW  - ABSORPTION-SPECTRA
KW  - COFACTOR FLAVIN
KW  - BASIS-SETS
KW  - RIBOFLAVIN
KW  - PHOTOTROPINFMN
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/cp/c9cp04918e
N2  - Flavin mononucleotide (FMN) belongs to the large family of flavins, ubiquitous yellow-coloured biological chromophores that contain an isoalloxazine ring system. As a cofactor in flavoproteins, it is found in various enzymes and photosensory receptors, like those featuring the light-oxygen-voltage (LOV) domain. The photocycle of FMN is triggered by blue light and proceeds via a cascade of intermediate states. In this work, we have studied isolated FMN in an aqueous solution in order to elucidate the intrinsic electronic and vibrational changes of the chromophore upon excitation. The ultrafast transitions of excited FMN were monitored through the joint use of femtosecond stimulated Raman spectroscopy (FSRS) and transient absorption spectroscopy encompassing a time window between 0 ps and 6 ns with 50 fs time resolution. Global analysis of the obtained transient visible absorption and transient Raman spectra in combination with extensive quantum chemistry calculations identified unambiguously the singlet and triplet FMN populations and addressed solvent dynamics effects. The good agreement between the experimental and theoretical spectra facilitated the assignment of electronic transitions and vibrations. Our results represent the first steps towards more complex experiments aimed at tracking structural changes of FMN embedded in light-inducible proteins upon photoexcitation.
ER  -

ANDRIKOPOULOS, Prokopis C; Yingliang LIU; Alessandra PICCHIOTTI; Nils LENNGREN; Miroslav KLOZ; Aditya S CHAUDHARI; Martin PRECEK; Mateusz REBARZ; Jakob ANDREASSON; Janos HAJDU; Bohdan SCHNEIDER a Gustavo FUERTES. Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations. \textit{Physical Chemistry Chemical Physics}. CAMBRIDGE: Royal Society of Chemistry, 2020, roč.~22, č.~12, s.~6538-6552. ISSN~1463-9076. Dostupné z: https://doi.org/10.1039/c9cp04918e.

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