Photoinduced damage of AsLOV2 domain is accompanied by
increased singlet oxygen production due to flavin dissociation

J 2020

Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation

PETRENCAKOVA, Martina; Frantisek FILANDR; Andrej HOVAN; Ghazaleh YASSAGHI; Petr MAN et al.

Základní údaje

Originální název

Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation

Autoři

Vydání

Nature Scientific Reports, BERLIN, NATURE PORTFOLIO, 2020, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.380

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90043/20:00139233

Organizační jednotka

CIISB

Klíčová slova anglicky

ATOM FORCE-FIELD; PROTEIN SECONDARY STRUCTURE; OXIDATIVE MODIFICATION; CYTOCHROME-CLOV2 DOMAIN; GENERATION; MINISOG; PHOTOSENSITIZATION; INACTIVATION; ENVIRONMENT

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 3. 2025 09:09, Mgr. Eva Dubská

Anotace

V originále

Flavin mononucleotide (FMN) belongs to the group of very efficient endogenous photosensitizers producing singlet oxygen, O-1(2), but with limited ability to be targeted. On the other hand, in geneticallyencoded photosensitizers, which can be targeted by means of various tags, the efficiency of FMN to produce O-1(2) is significantly diminished due to its interactions with surrounding amino acid residues. Recently, an increase of O-1(2) production yield by FMN buried in a protein matrix was achieved by a decrease of quenching of the cofactor excited states by weakening of the protein-FMN interactions while still forming a complex. Here, we suggest an alternative approach which relies on the blue light irradiation-induced dissociation of FMN to solvent. This dissociation unlocks the full capacity of FMN as O-1(2) producer. Our suggestion is based on the study of an irradiation effect on two variants of the LOV2 domain from Avena sativa; wild type, AsLOV2 wt, and the variant with a replaced cysteine residue, AsLOV2 C450A. We detected irradiation-induced conformational changes as well as oxidation of several amino acids in both AsLOV2 variants. Detailed analysis of these observations indicates that irradiationinduced increase in O-1(2) production is caused by a release of FMN from the protein. Moreover, an increased FMN dissociation from AsLOV2 wt in comparison with AsLOV2 C450A points to a role of C450 oxidation in repelling the cofactor from the protein.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

PETRENCAKOVA, Martina; Frantisek FILANDR; Andrej HOVAN; Ghazaleh YASSAGHI; Petr MAN; Tibor KOZAR; Marc-Simon SCHWER; Daniel JANCURA; Andreas PLUCKTHUN; Petr NOVAK; Pavol MISKOVSKY; Gregor BANO a Erik SEDLAK. Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation. Nature Scientific Reports. BERLIN: NATURE PORTFOLIO, 2020, roč. 10, č. 1, s. 1-15. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-020-60861-2.

@article{2487403,
   author = {Petrencakova, Martina and Filandr, Frantisek and Hovan, Andrej and Yassaghi, Ghazaleh and Man, Petr and Kozar, Tibor and Schwer, MarcandSimon and Jancura, Daniel and Pluckthun, Andreas and Novak, Petr and Miskovsky, Pavol and Bano, Gregor and Sedlak, Erik},
   article_location = {BERLIN},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-020-60861-2},
   keywords = {ATOM FORCE-FIELD; PROTEIN SECONDARY STRUCTURE; OXIDATIVE MODIFICATION; CYTOCHROME-CLOV2 DOMAIN; GENERATION; MINISOG; PHOTOSENSITIZATION; INACTIVATION; ENVIRONMENT},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation},
   url = {https://www.nature.com/articles/s41598-020-60861-2},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 2487403
AU  - Petrencakova, Martina - Filandr, Frantisek - Hovan, Andrej - Yassaghi, Ghazaleh - Man, Petr - Kozar, Tibor - Schwer, Marc-Simon - Jancura, Daniel - Pluckthun, Andreas - Novak, Petr - Miskovsky, Pavol - Bano, Gregor - Sedlak, Erik
PY  - 2020
TI  - Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation
JF  - Nature Scientific Reports
VL  - 10
IS  - 1
SP  - 1-15
EP  - 1-15
PB  - NATURE PORTFOLIO
SN  - 20452322
KW  - ATOM FORCE-FIELD
KW  - PROTEIN SECONDARY STRUCTURE
KW  - OXIDATIVE MODIFICATION
KW  - CYTOCHROME-CLOV2 DOMAIN
KW  - GENERATION
KW  - MINISOG
KW  - PHOTOSENSITIZATION
KW  - INACTIVATION
KW  - ENVIRONMENT
UR  - https://www.nature.com/articles/s41598-020-60861-2
N2  - Flavin mononucleotide (FMN) belongs to the group of very efficient endogenous photosensitizers producing singlet oxygen, O-1(2), but with limited ability to be targeted. On the other hand, in geneticallyencoded photosensitizers, which can be targeted by means of various tags, the efficiency of FMN to produce O-1(2) is significantly diminished due to its interactions with surrounding amino acid residues. Recently, an increase of O-1(2) production yield by FMN buried in a protein matrix was achieved by a decrease of quenching of the cofactor excited states by weakening of the protein-FMN interactions while still forming a complex. Here, we suggest an alternative approach which relies on the blue light irradiation-induced dissociation of FMN to solvent. This dissociation unlocks the full capacity of FMN as O-1(2) producer. Our suggestion is based on the study of an irradiation effect on two variants of the LOV2 domain from Avena sativa; wild type, AsLOV2 wt, and the variant with a replaced cysteine residue, AsLOV2 C450A. We detected irradiation-induced conformational changes as well as oxidation of several amino acids in both AsLOV2 variants. Detailed analysis of these observations indicates that irradiationinduced increase in O-1(2) production is caused by a release of FMN from the protein. Moreover, an increased FMN dissociation from AsLOV2 wt in comparison with AsLOV2 C450A points to a role of C450 oxidation in repelling the cofactor from the protein.
ER  -

PETRENCAKOVA, Martina; Frantisek FILANDR; Andrej HOVAN; Ghazaleh YASSAGHI; Petr MAN; Tibor KOZAR; Marc-Simon SCHWER; Daniel JANCURA; Andreas PLUCKTHUN; Petr NOVAK; Pavol MISKOVSKY; Gregor BANO a Erik SEDLAK. Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation. \textit{Nature Scientific Reports}. BERLIN: NATURE PORTFOLIO, 2020, roč.~10, č.~1, s.~1-15. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-020-60861-2.

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