De novo developed protein binders mimicking Interferon
lambda signaling

J 2022

De novo developed protein binders mimicking Interferon lambda signaling

KOLAROVA, Lucie; Jiri ZAHRADNIK; Maros HULICIAK; Pavel MIKULECKY; Yoav PELEG et al.

Základní údaje

Originální název

De novo developed protein binders mimicking Interferon lambda signaling

Autoři

KOLAROVA, Lucie; Jiri ZAHRADNIK; Maros HULICIAK; Pavel MIKULECKY; Yoav PELEG; Maya SHEMESH; Gideon SCHREIBER a Bohdan SCHNEIDER

Vydání

FEBS Journal, MALDEN, Blackwell, 2022, 1742-464X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90127/22:00139239

Organizační jednotka

CIISB II

Klíčová slova anglicky

cytokine signaling; cytokines; directed evolution; IFN-lambda; IL-29; interferon lambda; protein scaffolds; yeast display

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 3. 2025 14:57, Mgr. Eva Dubská

Anotace

V originále

We hereby describe the process of design and selection of nonantibody protein binders mimicking cytokine signaling. We chose to mimic signaling of IFN-lambda 1, type 3 interferon (also known as IL-29) for its novelty and the importance of its biological functions. All four known interferons lambda signal through binding to the extracellular domains of IL-28 receptor 1 (IL-28R1) and IL-10 receptor 2 (IL-10R2). Our binders were therefore trained to bind both receptors simultaneously. The bifunctional binder molecules were developed by yeast display, a method of directed evolution. The signaling capacity of the bivalent binders was tested by measuring phosphorylation of the JAK/STAT signaling pathway and production of mRNA of six selected genes naturally induced by IFN- lambda 1 in human cell lines. The newly developed bivalent binders offer opportunities to study cytokine-related biological functions and modulation of the cell behavior by receptor activation on the cell surfaces alternative to the use of natural IFN-lambda.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KOLAROVA, Lucie; Jiri ZAHRADNIK; Maros HULICIAK; Pavel MIKULECKY; Yoav PELEG; Maya SHEMESH; Gideon SCHREIBER a Bohdan SCHNEIDER. De novo developed protein binders mimicking Interferon lambda signaling. FEBS Journal. MALDEN: Blackwell, 2022, roč. 289, č. 9, s. 2672-2684. ISSN 1742-464X. Dostupné z: https://doi.org/10.1111/febs.16300.

@article{2487421,
 author = {Kolarova, Lucie and Zahradnik, Jiri and Huliciak, Maros and Mikulecky, Pavel and Peleg, Yoav and Shemesh, Maya and Schreiber, Gideon and Schneider, Bohdan},
 article_location = {MALDEN},
 article_number = {9},
 doi = {https://doi.org/10.1111/febs.16300},
 keywords = {cytokine signaling; cytokines; directed evolution; IFN-lambda; IL-29; interferon lambda; protein scaffolds; yeast display},
 language = {eng},
 issn = {1742-464X},
 journal = {FEBS Journal},
 title = {De novo developed protein binders mimicking Interferon lambda signaling},
 url = {https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16300},
 volume = {289},
 year = {2022}
}

TY - JOUR
ID - 2487421
AU - Kolarova, Lucie - Zahradnik, Jiri - Huliciak, Maros - Mikulecky, Pavel - Peleg, Yoav - Shemesh, Maya - Schreiber, Gideon - Schneider, Bohdan
PY - 2022
TI - De novo developed protein binders mimicking Interferon lambda signaling
JF - FEBS Journal
VL - 289
IS - 9
SP - 2672-2684
EP - 2672-2684
PB - Blackwell
SN - 1742464X
KW - cytokine signaling
KW - cytokines
KW - directed evolution
KW - IFN-lambda
KW - IL-29
KW - interferon lambda
KW - protein scaffolds
KW - yeast display
UR - https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16300
N2 - We hereby describe the process of design and selection of nonantibody protein binders mimicking cytokine signaling. We chose to mimic signaling of IFN-lambda 1, type 3 interferon (also known as IL-29) for its novelty and the importance of its biological functions. All four known interferons lambda signal through binding to the extracellular domains of IL-28 receptor 1 (IL-28R1) and IL-10 receptor 2 (IL-10R2). Our binders were therefore trained to bind both receptors simultaneously. The bifunctional binder molecules were developed by yeast display, a method of directed evolution. The signaling capacity of the bivalent binders was tested by measuring phosphorylation of the JAK/STAT signaling pathway and production of mRNA of six selected genes naturally induced by IFN- lambda 1 in human cell lines. The newly developed bivalent binders offer opportunities to study cytokine-related biological functions and modulation of the cell behavior by receptor activation on the cell surfaces alternative to the use of natural IFN-lambda.
ER -

KOLAROVA, Lucie; Jiri ZAHRADNIK; Maros HULICIAK; Pavel MIKULECKY; Yoav PELEG; Maya SHEMESH; Gideon SCHREIBER a Bohdan SCHNEIDER. \&{}lt;i\&{}gt;De novo\&{}lt;/i\&{}gt; developed protein binders mimicking Interferon lambda signaling. \textit{FEBS Journal}. MALDEN: Blackwell, 2022, roč.~289, č.~9, s.~2672-2684. ISSN~1742-464X. Dostupné z: https://doi.org/10.1111/febs.16300.

Přehled o publikaci

<i>De novo</i> developed protein binders mimicking Interferon lambda signaling