Overlapping and separable activities of BRA-2 and HIM-17
promote occurrence and regulation of pairing and synapsis
during Caenorhabditis elegans meiosis

J 2025

Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis

BLAŽÍČKOVÁ, Jitka; Shalini TRIVEDI; Richard BOWMAN; Sowmya Sivakumar GEETHA; Silma SUBAH et al.

Základní údaje

Originální název

Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis

Autoři

Vydání

Nature Communications, Berlin, Nature Research, 2025, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 15.700 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00140941

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

BRA-2; HIM-17; meiosis; pairing; synapsis

Štítky

14110513, CF CELLIM, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2026 07:59, Mgr. Tereza Miškechová

Anotace

V originále

Faithful meiotic segregation requires pairwise alignment of the homologous chromosomes and their synaptonemal complex (SC) mediated stabilization. Here, we investigate factors that promote and coordinate these events during C. elegans meiosis. We identify BRA-2 (BMP Receptor Associated family member 2) as an interactor of HIM-17, previously shown to promote double-strand break formation. We found that loss of bra-2 impairs synapsis elongation without affecting homolog recognition, chromosome movement or SC maintenance. Epistasis analyses reveal previously unrecognized activities for HIM-17 in regulating homolog pairing and SC assembly in a partially overlapping manner with BRA-2. We show that removing bra-2 or him-17 restores nuclear clustering, recruitment of PLK-2 at the nuclear periphery, and abrogation of ectopic synapsis in htp-1 mutants, suggesting intact CHK-2-mediated signaling and presence of a barrier that prevents SC polymerization in the absence of homology. Our findings shed light on the regulatory mechanisms ensuring faithful pairing and synapsis.

Návaznosti

GA23-04918S, projekt VaV

Název: Mechanismus založený na modifikaci chromatinu osvětluje novou dráhu pro vytvoření meiotické synapse chromozomů

Investor: Grantová agentura ČR, Mechanismus založený na modifikaci chromatinu osvětluje novou dráhu pro vytvoření meiotické synapse chromozomů

90242, velká výzkumná infrastruktura

Název: CIISB III

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

BLAŽÍČKOVÁ, Jitka; Shalini TRIVEDI; Richard BOWMAN; Sowmya Sivakumar GEETHA; Silma SUBAH; Michelle SCUZZARELLA; Alexander CHANG; Uma R CHANDRAN; Judith L YANOWITZ; Sarit SMOLIKOVE; Verena JANTSCH; Monique ZETKA a Nicola SILVA. Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis. Nature Communications. Berlin: Nature Research, 2025, roč. 16, č. 1, s. 1-20. ISSN 2041-1723. Dostupné z: https://doi.org/10.1038/s41467-025-57862-y.

@article{2488520,
   author = {Blažíčková, Jitka and Trivedi, Shalini and Bowman, Richard and Geetha, Sowmya Sivakumar and Subah, Silma and Scuzzarella, Michelle and Chang, Alexander and Chandran, Uma R and Yanowitz, Judith L and Smolikove, Sarit and Jantsch, Verena and Zetka, Monique and Silva, Nicola},
   article_location = {Berlin},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41467-025-57862-y},
   keywords = {BRA-2; HIM-17; meiosis; pairing; synapsis},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis},
   url = {https://www.nature.com/articles/s41467-025-57862-y},
   volume = {16},
   year = {2025}
}

TY  - JOUR
ID  - 2488520
AU  - Blažíčková, Jitka - Trivedi, Shalini - Bowman, Richard - Geetha, Sowmya Sivakumar - Subah, Silma - Scuzzarella, Michelle - Chang, Alexander - Chandran, Uma R - Yanowitz, Judith L - Smolikove, Sarit - Jantsch, Verena - Zetka, Monique - Silva, Nicola
PY  - 2025
TI  - Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis
JF  - Nature Communications
VL  - 16
IS  - 1
SP  - 1-20
EP  - 1-20
PB  - Nature Research
SN  - 20411723
KW  - BRA-2
KW  - HIM-17
KW  - meiosis
KW  - pairing
KW  - synapsis
UR  - https://www.nature.com/articles/s41467-025-57862-y
N2  - Faithful meiotic segregation requires pairwise alignment of the homologous chromosomes and their synaptonemal complex (SC) mediated stabilization. Here, we investigate factors that promote and coordinate these events during C. elegans meiosis. We identify BRA-2 (BMP Receptor Associated family member 2) as an interactor of HIM-17, previously shown to promote double-strand break formation. We found that loss of bra-2 impairs synapsis elongation without affecting homolog recognition, chromosome movement or SC maintenance. Epistasis analyses reveal previously unrecognized activities for HIM-17 in regulating homolog pairing and SC assembly in a partially overlapping manner with BRA-2. We show that removing bra-2 or him-17 restores nuclear clustering, recruitment of PLK-2 at the nuclear periphery, and abrogation of ectopic synapsis in htp-1 mutants, suggesting intact CHK-2-mediated signaling and presence of a barrier that prevents SC polymerization in the absence of homology. Our findings shed light on the regulatory mechanisms ensuring faithful pairing and synapsis.
ER  -

BLAŽÍČKOVÁ, Jitka; Shalini TRIVEDI; Richard BOWMAN; Sowmya Sivakumar GEETHA; Silma SUBAH; Michelle SCUZZARELLA; Alexander CHANG; Uma R CHANDRAN; Judith L YANOWITZ; Sarit SMOLIKOVE; Verena JANTSCH; Monique ZETKA a Nicola SILVA. Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis. \textit{Nature Communications}. Berlin: Nature Research, 2025, roč.~16, č.~1, s.~1-20. ISSN~2041-1723. Dostupné z: https://doi.org/10.1038/s41467-025-57862-y.

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