Urinary Metabolomic Profile in Children with Autism Spectrum
Disorder

J 2025

Urinary Metabolomic Profile in Children with Autism Spectrum Disorder

OSREDKAR, Josko; Kristina KUMER; Uros GODNOV; Maja Jekovec VRHOVSEK; Veronika VIDOVÁ et al.

Základní údaje

Originální název

Urinary Metabolomic Profile in Children with Autism Spectrum Disorder

Autoři

OSREDKAR, Josko; Kristina KUMER; Uros GODNOV; Maja Jekovec VRHOVSEK; Veronika VIDOVÁ; Elliott James PRICE; Tara JAVORNIK; Gorazd AVGUSTIN a Teja FABJAN

Vydání

International Journal of Molecular Sciences, BASEL, Molecular Diversity Preservation International, 2025, 1661-6596

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.900 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00140935

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

autism spectrum disorder; tryptophan; kynurenine; CARS

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2026 14:04, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children with ASD and 44 healthy children, members of the same family. The average age in the ASD group was 10.7 years, while the average age in the control group was 9.4 years. Urinary tryptophan metabolites were quantified via liquid chromatography-mass spectrometry operating multiple reaction monitoring (MRM). Urinary creatinine was analyzed on an Advia 2400 analyzer using the Jaffe reaction. Statistical comparisons were made between ASD subgroups based on CARS scores. Our findings indicate that children with ASD have higher TRP concentrations (19.94 vs. 16.91; p = 0.04) than their siblings. Kynurenine (KYN) was found at higher levels in children with ASD compared to children in the control group (82.34 vs. 71.20; p = 0.86), although this difference was not statistically significant. The ASD group showed trends of higher KYN/TRP ratios and altered TRP/ indole-3-acetic acid (IAA) and TRP/5-hydroxyindoleacetic acid (5-HIAA) ratios, correlating with symptom severity. Although the numbers of the two groups were different, our findings suggest that mild and severe illnesses involve separate mechanisms. However, further comprehensive studies are needed to validate these ratios as diagnostic tools for ASD.

Návaznosti

90269, velká výzkumná infrastruktura

Název: RECETOX RI II

Citovat

OSREDKAR, Josko; Kristina KUMER; Uros GODNOV; Maja Jekovec VRHOVSEK; Veronika VIDOVÁ; Elliott James PRICE; Tara JAVORNIK; Gorazd AVGUSTIN a Teja FABJAN. Urinary Metabolomic Profile in Children with Autism Spectrum Disorder. International Journal of Molecular Sciences. BASEL: Molecular Diversity Preservation International, 2025, roč. 26, č. 5, s. 1-17. ISSN 1661-6596. Dostupné z: https://doi.org/10.3390/ijms26052254.

@article{2488610,
   author = {Osredkar, Josko and Kumer, Kristina and Godnov, Uros and Vrhovsek, Maja Jekovec and Vidová, Veronika and Price, Elliott James and Javornik, Tara and Avgustin, Gorazd and Fabjan, Teja},
   article_location = {BASEL},
   article_number = {5},
   doi = {https://doi.org/10.3390/ijms26052254},
   keywords = {autism spectrum disorder; tryptophan; kynurenine; CARS},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Urinary Metabolomic Profile in Children with Autism Spectrum Disorder},
   url = {https://www.mdpi.com/1422-0067/26/5/2254},
   volume = {26},
   year = {2025}
}

TY  - JOUR
ID  - 2488610
AU  - Osredkar, Josko - Kumer, Kristina - Godnov, Uros - Vrhovsek, Maja Jekovec - Vidová, Veronika - Price, Elliott James - Javornik, Tara - Avgustin, Gorazd - Fabjan, Teja
PY  - 2025
TI  - Urinary Metabolomic Profile in Children with Autism Spectrum Disorder
JF  - International Journal of Molecular Sciences
VL  - 26
IS  - 5
SP  - 1-17
EP  - 1-17
PB  - Molecular Diversity Preservation International
SN  - 16616596
KW  - autism spectrum disorder
KW  - tryptophan
KW  - kynurenine
KW  - CARS
UR  - https://www.mdpi.com/1422-0067/26/5/2254
N2  - Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children with ASD and 44 healthy children, members of the same family. The average age in the ASD group was 10.7 years, while the average age in the control group was 9.4 years. Urinary tryptophan metabolites were quantified via liquid chromatography-mass spectrometry operating multiple reaction monitoring (MRM). Urinary creatinine was analyzed on an Advia 2400 analyzer using the Jaffe reaction. Statistical comparisons were made between ASD subgroups based on CARS scores. Our findings indicate that children with ASD have higher TRP concentrations (19.94 vs. 16.91; p = 0.04) than their siblings. Kynurenine (KYN) was found at higher levels in children with ASD compared to children in the control group (82.34 vs. 71.20; p = 0.86), although this difference was not statistically significant. The ASD group showed trends of higher KYN/TRP ratios and altered TRP/ indole-3-acetic acid (IAA) and TRP/5-hydroxyindoleacetic acid (5-HIAA) ratios, correlating with symptom severity. Although the numbers of the two groups were different, our findings suggest that mild and severe illnesses involve separate mechanisms. However, further comprehensive studies are needed to validate these ratios as diagnostic tools for ASD.
ER  -

OSREDKAR, Josko; Kristina KUMER; Uros GODNOV; Maja Jekovec VRHOVSEK; Veronika VIDOVÁ; Elliott James PRICE; Tara JAVORNIK; Gorazd AVGUSTIN a Teja FABJAN. Urinary Metabolomic Profile in Children with Autism Spectrum Disorder. \textit{International Journal of Molecular Sciences}. BASEL: Molecular Diversity Preservation International, 2025, roč.~26, č.~5, s.~1-17. ISSN~1661-6596. Dostupné z: https://doi.org/10.3390/ijms26052254.

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