Adapting the Laser-Induced Fluorescence Detection Setup of the
Standard Capillary Electrophoresis Equipment to Achieve
High-Sensitivity Detection of 2-Aminoacridone Labeled
Oligosaccharides

J 2025

Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides

DUŠA, Filip; Marcelina RUSIN; Denisa SMOLKOVÁ; Jozef SESTAK; Justyna DOBROWOLSKA-IWANEK et al.

Základní údaje

Originální název

Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides

Autoři

DUŠA, Filip; Marcelina RUSIN; Denisa SMOLKOVÁ; Jozef SESTAK; Justyna DOBROWOLSKA-IWANEK; Michal WOZNIAKIEWICZ a Jana LAVICKÁ

Vydání

Journal of Separation Science, Weinheim, Wiley, 2025, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL, URL

Impakt faktor

Impact factor: 2.800 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

2-aminoacridone; capillary electrophoresis; human milk oligosaccharides; laser-induced fluorescence

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 12. 2025 10:06, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.

Citovat

DUŠA, Filip; Marcelina RUSIN; Denisa SMOLKOVÁ; Jozef SESTAK; Justyna DOBROWOLSKA-IWANEK; Michal WOZNIAKIEWICZ a Jana LAVICKÁ. Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides. Journal of Separation Science. Weinheim: Wiley, 2025, roč. 48, č. 3, s. "e70112", 7 s. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.70112.

@article{2492937,
   author = {Duša, Filip and Rusin, Marcelina and Smolková, Denisa and Sestak, Jozef and DobrowolskaandIwanek, Justyna and Wozniakiewicz, Michal and Lavická, Jana},
   article_location = {Weinheim},
   article_number = {3},
   doi = {https://doi.org/10.1002/jssc.70112},
   keywords = {2-aminoacridone; capillary electrophoresis; human milk oligosaccharides; laser-induced fluorescence},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.70112},
   volume = {48},
   year = {2025}
}

TY  - JOUR
ID  - 2492937
AU  - Duša, Filip - Rusin, Marcelina - Smolková, Denisa - Sestak, Jozef - Dobrowolska-Iwanek, Justyna - Wozniakiewicz, Michal - Lavická, Jana
PY  - 2025
TI  - Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides
JF  - Journal of Separation Science
VL  - 48
IS  - 3
SP  - "e70112"
EP  - "e70112"
PB  - Wiley
SN  - 16159306
KW  - 2-aminoacridone
KW  - capillary electrophoresis
KW  - human milk oligosaccharides
KW  - laser-induced fluorescence
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.70112
N2  - The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.
ER  -

DUŠA, Filip; Marcelina RUSIN; Denisa SMOLKOVÁ; Jozef SESTAK; Justyna DOBROWOLSKA-IWANEK; Michal WOZNIAKIEWICZ a Jana LAVICKÁ. Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides. \textit{Journal of Separation Science}. Weinheim: Wiley, 2025, roč.~48, č.~3, s.~''e70112'', 7 s. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.70112.

Přehled o publikaci