Repeated Administrations of Polyphenolic Extracts Prevent
Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses
by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in
Spinal Cord-Injured Female Mice

J 2025

Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice

BAGÓ MAS, Anna; Andrea KORIMOVÁ; Karolína BRETOVÁ; Meritxell DEULOFEU; Enrique VERDU et al.

Základní údaje

Originální název

Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice

Autoři

BAGÓ MAS, Anna; Andrea KORIMOVÁ; Karolína BRETOVÁ; Meritxell DEULOFEU; Enrique VERDU; Nuria FIOL; Petr DUBOVÝ a Pere BOADAS-VAELLO

Vydání

International Journal of Molecular Sciences, BASEL, MDPI, 2025, 1661-6596

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.900 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

spinal cord injury; chronic neuropathic pain; neuroinflammation; polyphenols; glia; chemokines

Štítky

14110514, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 4. 2026 09:47, Mgr. Tereza Miškechová

Anotace

V originále

Neuropathic pain after spinal cord injury lacks any effective treatments, often leading to chronic pain. This study tested whether the daily administration of fully characterized polyphenolic extracts from grape stalks and coffee could prevent both reflexive and non-reflexive chronic neuropathic pain in spinal cord-injured mice by modulating the neuroimmune axis. Female CD1 mice underwent mild spinal cord contusion and received intraperitoneal extracts in weeks one, three, and six post-surgery. Reflexive pain responses were assessed weekly for up to 10 weeks, and non-reflexive pain was evaluated at the study's end. Neuroimmune crosstalk was investigated, focusing on glial activation and the expression of CCL2/CCR2 and CX3CL1/CX3CR1 in supraspinal pain-related areas, including the periaqueductal gray, rostral ventromedial medulla, anterior cingulate cortex, and amygdala. Repeated treatments prevented mechanical allodynia and thermal hyperalgesia, and also modulated non-reflexive pain. Moreover, they reduced supraspinal gliosis and regulated CCL2/CCR2 and CX3CL1/CX3CR1 signaling. Overall, the combination of polyphenols in these extracts may offer a promising pharmacological strategy to prevent chronic reflexive and non-reflexive pain responses by modifying central sensitization markers, not only at the contusion site but also in key supraspinal regions implicated in neuropathic pain. Overall, these data highlight the potential of polyphenolic extracts for spinal cord injury-induced chronic neuropathic pain.

Návaznosti

MUNI/A/1563/2023, interní kód MU

Název: Funkční morfologie: od molekulární biologie ke klinické anatomii 3

Investor: Masarykova univerzita, Funkční morfologie: od molekulární biologie ke klinické anatomii 3

Citovat

BAGÓ MAS, Anna; Andrea KORIMOVÁ; Karolína BRETOVÁ; Meritxell DEULOFEU; Enrique VERDU; Nuria FIOL; Petr DUBOVÝ a Pere BOADAS-VAELLO. Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice. International Journal of Molecular Sciences. BASEL: MDPI, 2025, roč. 26, č. 7, s. 1-30. ISSN 1661-6596. Dostupné z: https://doi.org/10.3390/ijms26073325.

@article{2494897,
   author = {Bagó Mas, Anna and Korimová, Andrea and Bretová, Karolína and Deulofeu, Meritxell and Verdu, Enrique and Fiol, Nuria and Dubový, Petr and BoadasandVaello, Pere},
   article_location = {BASEL},
   article_number = {7},
   doi = {https://doi.org/10.3390/ijms26073325},
   keywords = {spinal cord injury; chronic neuropathic pain; neuroinflammation; polyphenols; glia; chemokines},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice},
   url = {https://www.mdpi.com/1422-0067/26/7/3325},
   volume = {26},
   year = {2025}
}

TY  - JOUR
ID  - 2494897
AU  - Bagó Mas, Anna - Korimová, Andrea - Bretová, Karolína - Deulofeu, Meritxell - Verdu, Enrique - Fiol, Nuria - Dubový, Petr - Boadas-Vaello, Pere
PY  - 2025
TI  - Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice
JF  - International Journal of Molecular Sciences
VL  - 26
IS  - 7
SP  - 1-30
EP  - 1-30
PB  - MDPI
SN  - 16616596
KW  - spinal cord injury
KW  - chronic neuropathic pain
KW  - neuroinflammation
KW  - polyphenols
KW  - glia
KW  - chemokines
UR  - https://www.mdpi.com/1422-0067/26/7/3325
N2  - Neuropathic pain after spinal cord injury lacks any effective treatments, often leading to chronic pain. This study tested whether the daily administration of fully characterized polyphenolic extracts from grape stalks and coffee could prevent both reflexive and non-reflexive chronic neuropathic pain in spinal cord-injured mice by modulating the neuroimmune axis. Female CD1 mice underwent mild spinal cord contusion and received intraperitoneal extracts in weeks one, three, and six post-surgery. Reflexive pain responses were assessed weekly for up to 10 weeks, and non-reflexive pain was evaluated at the study's end. Neuroimmune crosstalk was investigated, focusing on glial activation and the expression of CCL2/CCR2 and CX3CL1/CX3CR1 in supraspinal pain-related areas, including the periaqueductal gray, rostral ventromedial medulla, anterior cingulate cortex, and amygdala. Repeated treatments prevented mechanical allodynia and thermal hyperalgesia, and also modulated non-reflexive pain. Moreover, they reduced supraspinal gliosis and regulated CCL2/CCR2 and CX3CL1/CX3CR1 signaling. Overall, the combination of polyphenols in these extracts may offer a promising pharmacological strategy to prevent chronic reflexive and non-reflexive pain responses by modifying central sensitization markers, not only at the contusion site but also in key supraspinal regions implicated in neuropathic pain. Overall, these data highlight the potential of polyphenolic extracts for spinal cord injury-induced chronic neuropathic pain.
ER  -

BAGÓ MAS, Anna; Andrea KORIMOVÁ; Karolína BRETOVÁ; Meritxell DEULOFEU; Enrique VERDU; Nuria FIOL; Petr DUBOVÝ a Pere BOADAS-VAELLO. Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice. \textit{International Journal of Molecular Sciences}. BASEL: MDPI, 2025, roč.~26, č.~7, s.~1-30. ISSN~1661-6596. Dostupné z: https://doi.org/10.3390/ijms26073325.

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