Interaction process behind the strong stabilization of
G-quadruplexes by alkaloid fagaronine

J 2025

Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine

HANNIG, Pavel; Raimundo GARGALLO; Stefania MAZZINI; Gigliola BORGONOVO; Marco ZUCCOLO et al.

Základní údaje

Originální název

Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine

Autoři

HANNIG, Pavel; Raimundo GARGALLO; Stefania MAZZINI; Gigliola BORGONOVO; Marco ZUCCOLO; Eva TÁBORSKÁ a Petr TÁBORSKÝ

Vydání

Biophysical Chemistry, AMSTERDAM, Elsevier, 2025, 0301-4622

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Fagaronine; G-quadruplex; Fluorescence spectroscopy; Circular dichroism spectroscopy; NMR spectroscopy; Docking simulation

Štítky

14110512, 14313010, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 9. 2025 09:02, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Benzo[c]phenanthridine alkaloids are known for their stabilizing effects on non-canonical DNA structures, particularly G-quadruplexes (G4s). In this study, the interaction of fagaronine, a rare benzo[c]phenanthridine alkaloid, with several DNA structures (including B-DNA, parallel, antiparallel and hybrid G4s) is studied using molecular fluorescence and circular dichroism (CD) spectroscopy. It has been found that fagaronine significantly enhances the stability of all tested G4 conformations. Furthermore, a study by NMR spectroscopy provided valuable information on the mechanism of interaction of the ligand with the parallel G4 structure adopted by Pu22T14T23, a sequence mutated with respect to that found within the promoter region of the c-myc gene. Remarkably, when compared with data reported in the literature, fagaronine appears to exhibit one of the strongest G4 thermal stabilization effects ever recorded for a small ligand.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

MUNI/A/1594/2023, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2024

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2024

Citovat

HANNIG, Pavel; Raimundo GARGALLO; Stefania MAZZINI; Gigliola BORGONOVO; Marco ZUCCOLO; Eva TÁBORSKÁ a Petr TÁBORSKÝ. Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine. Biophysical Chemistry. AMSTERDAM: Elsevier, 2025, roč. 323, August 2025, s. 1-12. ISSN 0301-4622. Dostupné z: https://doi.org/10.1016/j.bpc.2025.107443.

@article{2494917,
   author = {Hannig, Pavel and Gargallo, Raimundo and Mazzini, Stefania and Borgonovo, Gigliola and Zuccolo, Marco and Táborská, Eva and Táborský, Petr},
   article_location = {AMSTERDAM},
   article_number = {August 2025},
   doi = {https://doi.org/10.1016/j.bpc.2025.107443},
   keywords = {Fagaronine; G-quadruplex; Fluorescence spectroscopy; Circular dichroism spectroscopy; NMR spectroscopy; Docking simulation},
   language = {eng},
   issn = {0301-4622},
   journal = {Biophysical Chemistry},
   title = {Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine},
   url = {https://www.sciencedirect.com/science/article/pii/S0301462225000559?via%3Dihub},
   volume = {323},
   year = {2025}
}

TY  - JOUR
ID  - 2494917
AU  - Hannig, Pavel - Gargallo, Raimundo - Mazzini, Stefania - Borgonovo, Gigliola - Zuccolo, Marco - Táborská, Eva - Táborský, Petr
PY  - 2025
TI  - Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine
JF  - Biophysical Chemistry
VL  - 323
IS  - August 2025
SP  - 1-12
EP  - 1-12
PB  - Elsevier
SN  - 03014622
KW  - Fagaronine
KW  - G-quadruplex
KW  - Fluorescence spectroscopy
KW  - Circular dichroism spectroscopy
KW  - NMR spectroscopy
KW  - Docking simulation
UR  - https://www.sciencedirect.com/science/article/pii/S0301462225000559?via%3Dihub
N2  - Benzo[c]phenanthridine alkaloids are known for their stabilizing effects on non-canonical DNA structures, particularly G-quadruplexes (G4s). In this study, the interaction of fagaronine, a rare benzo[c]phenanthridine alkaloid, with several DNA structures (including B-DNA, parallel, antiparallel and hybrid G4s) is studied using molecular fluorescence and circular dichroism (CD) spectroscopy. It has been found that fagaronine significantly enhances the stability of all tested G4 conformations. Furthermore, a study by NMR spectroscopy provided valuable information on the mechanism of interaction of the ligand with the parallel G4 structure adopted by Pu22T14T23, a sequence mutated with respect to that found within the promoter region of the c-myc gene. Remarkably, when compared with data reported in the literature, fagaronine appears to exhibit one of the strongest G4 thermal stabilization effects ever recorded for a small ligand.
ER  -

HANNIG, Pavel; Raimundo GARGALLO; Stefania MAZZINI; Gigliola BORGONOVO; Marco ZUCCOLO; Eva TÁBORSKÁ a Petr TÁBORSKÝ. Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine. \textit{Biophysical Chemistry}. AMSTERDAM: Elsevier, 2025, roč.~323, August 2025, s.~1-12. ISSN~0301-4622. Dostupné z: https://doi.org/10.1016/j.bpc.2025.107443.

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