Bioenergetics of human spermatozoa in patients with testicular
germ cell tumours

J 2025

Bioenergetics of human spermatozoa in patients with testicular germ cell tumours

SIMONIK, Ondrej; Barbora BRYNDOVA; Vishma Pratap SUR; Lukas DED; Zuzana COCKOVA et al.

Základní údaje

Originální název

Bioenergetics of human spermatozoa in patients with testicular germ cell tumours

Autoři

Vydání

Molecular Human Reproduction, OXFORD, OXFORD UNIV PRESS, 2025, 1360-9947

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.500 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00141128

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

2P-FLIM; TGCT; XF Analyzer; cancer; energetic metabolism; infertility; oxidative phosphorylation; sperm biochemistry; sperm function; spermatozoa

Štítky

14110240, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 7. 2025 08:34, Mgr. Tereza Miškechová

Anotace

V originále

In patients with testicular germ cell tumours (TGCT), sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated with a low sperm recovery rate after thawing. Since sperm is a high-energy demanding cell, which is supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in sperm anomalies. In this study, we investigated the bioenergetic pattern of cryopreserved sperm of TGCT patients in comparison with normozoospermic samples using two state-of-the-art methods: the Extracellular Flux Analyzer (XF Analyzer) and two-photon fluorescence lifetime imaging microscopy (2P-FLIM), in order to assess the contributions of OXPHOS and glycolysis to energy provision. A novel protocol for the combined measurement of OXPHOS (oxygen consumption rate: OCR) and glycolysis (extracellular acidification rate: ECAR) using the XF Analyzer was developed together with a unique customized AI-based approach for semiautomated processing of 2P-FLIM images. Our study delivers optimized low-HEPES modified human tubal fluid media (mHTF) for sperm handling during pre-analytical and analytical phases, to maintain sperm physiological parameters and optimal OCR, equivalent to OXPHOS. The negative effect of cryopreservation was signified by the deterioration of both bioenergetic pathways represented by modified OCR and ECAR curves and the derived parameters. This was true for normozoospermic as well as samples from TGCT patients, which showed even stronger damage within the respiratory chain compared to the level of glycolytic activity impairment. The impact of cryopreservation and pathology are supported by 2P-FLIM analysis, showing a significant decrease in bound NADH in contrast to unbound NAD(P)H, which reflects decreased metabolic activity in samples from TGCT patients. Our study provides novel insights into the impact of TGCT on sperm bioenergetics and delivers a verified protocol to be used for the assessment of human sperm metabolic activity, which can be a valuable tool for further research and clinical andrology.

Návaznosti

LM2023050, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech BioImaging: Národní výzkumná infrastruktura pro biologické a medicínské zobrazování

Citovat

SIMONIK, Ondrej; Barbora BRYNDOVA; Vishma Pratap SUR; Lukas DED; Zuzana COCKOVA; Ales BENDA; Maryam QASEMI; Petr PECINA; Alena PECINOVA; Daniela SPEVAKOVA; Tomas HRADEC; Pavel SKROBANEK; Zuzana EZROVA; Zuzana KRATKA; Radomir KREN; Michal JEŠETA; Ludmila BOUBLIKOVA; Libor ZAMECNIK; Tomas BÜCHLER; Jiri NEUZIL; Pavla POSTLEROVA a Katerina KOMRSKOVA. Bioenergetics of human spermatozoa in patients with testicular germ cell tumours. Molecular Human Reproduction. OXFORD: OXFORD UNIV PRESS, 2025, roč. 31, č. 1, s. 1-17. ISSN 1360-9947. Dostupné z: https://doi.org/10.1093/molehr/gaaf005.

@article{2495137,
   author = {Simonik, Ondrej and Bryndova, Barbora and Sur, Vishma Pratap and Ded, Lukas and Cockova, Zuzana and Benda, Ales and Qasemi, Maryam and Pecina, Petr and Pecinova, Alena and Spevakova, Daniela and Hradec, Tomas and Skrobanek, Pavel and Ezrova, Zuzana and Kratka, Zuzana and Kren, Radomir and Ješeta, Michal and Boublikova, Ludmila and Zamecnik, Libor and Büchler, Tomas and Neuzil, Jiri and Postlerova, Pavla and Komrskova, Katerina},
   article_location = {OXFORD},
   article_number = {1},
   doi = {https://doi.org/10.1093/molehr/gaaf005},
   keywords = {2P-FLIM; TGCT; XF Analyzer; cancer; energetic metabolism; infertility; oxidative phosphorylation; sperm biochemistry; sperm function; spermatozoa},
   language = {eng},
   issn = {1360-9947},
   journal = {Molecular Human Reproduction},
   title = {Bioenergetics of human spermatozoa in patients with testicular germ cell tumours},
   url = {https://academic.oup.com/molehr/article-abstract/31/1/gaaf005/8063628?redirectedFrom=fulltext&login=false},
   volume = {31},
   year = {2025}
}

TY  - JOUR
ID  - 2495137
AU  - Simonik, Ondrej - Bryndova, Barbora - Sur, Vishma Pratap - Ded, Lukas - Cockova, Zuzana - Benda, Ales - Qasemi, Maryam - Pecina, Petr - Pecinova, Alena - Spevakova, Daniela - Hradec, Tomas - Skrobanek, Pavel - Ezrova, Zuzana - Kratka, Zuzana - Kren, Radomir - Ješeta, Michal - Boublikova, Ludmila - Zamecnik, Libor - Büchler, Tomas - Neuzil, Jiri - Postlerova, Pavla - Komrskova, Katerina
PY  - 2025
TI  - Bioenergetics of human spermatozoa in patients with testicular germ cell tumours
JF  - Molecular Human Reproduction
VL  - 31
IS  - 1
SP  - 1-17
EP  - 1-17
PB  - OXFORD UNIV PRESS
SN  - 13609947
KW  - 2P-FLIM
KW  - TGCT
KW  - XF Analyzer
KW  - cancer
KW  - energetic metabolism
KW  - infertility
KW  - oxidative phosphorylation
KW  - sperm biochemistry
KW  - sperm function
KW  - spermatozoa
UR  - https://academic.oup.com/molehr/article-abstract/31/1/gaaf005/8063628?redirectedFrom=fulltext&login=false
N2  - In patients with testicular germ cell tumours (TGCT), sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated with a low sperm recovery rate after thawing. Since sperm is a high-energy demanding cell, which is supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in sperm anomalies. In this study, we investigated the bioenergetic pattern of cryopreserved sperm of TGCT patients in comparison with normozoospermic samples using two state-of-the-art methods: the Extracellular Flux Analyzer (XF Analyzer) and two-photon fluorescence lifetime imaging microscopy (2P-FLIM), in order to assess the contributions of OXPHOS and glycolysis to energy provision. A novel protocol for the combined measurement of OXPHOS (oxygen consumption rate: OCR) and glycolysis (extracellular acidification rate: ECAR) using the XF Analyzer was developed together with a unique customized AI-based approach for semiautomated processing of 2P-FLIM images. Our study delivers optimized low-HEPES modified human tubal fluid media (mHTF) for sperm handling during pre-analytical and analytical phases, to maintain sperm physiological parameters and optimal OCR, equivalent to OXPHOS. The negative effect of cryopreservation was signified by the deterioration of both bioenergetic pathways represented by modified OCR and ECAR curves and the derived parameters. This was true for normozoospermic as well as samples from TGCT patients, which showed even stronger damage within the respiratory chain compared to the level of glycolytic activity impairment. The impact of cryopreservation and pathology are supported by 2P-FLIM analysis, showing a significant decrease in bound NADH in contrast to unbound NAD(P)H, which reflects decreased metabolic activity in samples from TGCT patients. Our study provides novel insights into the impact of TGCT on sperm bioenergetics and delivers a verified protocol to be used for the assessment of human sperm metabolic activity, which can be a valuable tool for further research and clinical andrology.
ER  -

SIMONIK, Ondrej; Barbora BRYNDOVA; Vishma Pratap SUR; Lukas DED; Zuzana COCKOVA; Ales BENDA; Maryam QASEMI; Petr PECINA; Alena PECINOVA; Daniela SPEVAKOVA; Tomas HRADEC; Pavel SKROBANEK; Zuzana EZROVA; Zuzana KRATKA; Radomir KREN; Michal JEŠETA; Ludmila BOUBLIKOVA; Libor ZAMECNIK; Tomas BÜCHLER; Jiri NEUZIL; Pavla POSTLEROVA a Katerina KOMRSKOVA. Bioenergetics of human spermatozoa in patients with testicular germ cell tumours. \textit{Molecular Human Reproduction}. OXFORD: OXFORD UNIV PRESS, 2025, roč.~31, č.~1, s.~1-17. ISSN~1360-9947. Dostupné z: https://doi.org/10.1093/molehr/gaaf005.

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