Morphological characteristics, extracellular vesicle structure
and stem-like specificity of human follicular fluid cell
subpopulation during osteodifferentiation

J 2025

Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation

KRANC, Wiesława; Mariusz KACZMAREK; Katarzyna KOWALSKA; Wojciech PIEŃKOWSKI; Sylwia CIESIÓŁKA et al.

Základní údaje

Originální název

Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation

Autoři

Vydání

Experimental and Molecular Pathology, SAN DIEGO, ELSEVIER, 2025, 0014-4800

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00141129

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Human granulosa cells; Osteodifferentiation; Extracellular vesicles

Štítky

14110240, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 7. 2025 08:39, Mgr. Tereza Miškechová

Anotace

V originále

Extracellular vesicles can play an important role in the processes occurring after stem cell transplantation, preventing cell apoptosis, stimulating immunological processes, and promoting the synthesis of extracellular matrix. Human follicular fluid (FF) can be a source of a subpopulation of cells with mesenchymal stem cells (MSCs) properties. Moreover these subpopulations of FF cells can differentiate into osteoblasts. In presented studies flow cytometry of ovarian FF cells confirmed positive expression of MSCs markers such as: CD44, CD90, CD105, CD73 and negative expression of a hematopoietic marker: CD45. The CD90+, CD105+, CD45- cell subpopulation has been obtained during magnetic separation using appropriate antibodies conjugated with microbeads. The extracellular vesicles (EVs) secreted by the cells during osteodifferentiation process differed from those secreted by cells culture in the basal medium. Based on the previous and current electron microscopy research, changes in size, number, and shape would support the notion that released EVs could be crucial to the ovarian FF cell subpopulation differentiation process. Osteogenic differentiation has been confirmed via Alizarin red staining. Therefore, follicular fluid (FF) can be a new source of a cell subpopulation with MSC properties, with the cells capable of differentiating into the osteogenic lineage. EVs could play a key role as mediators in tissue regeneration, especially bone tissue regeneration.

Citovat

KRANC, Wiesława; Mariusz KACZMAREK; Katarzyna KOWALSKA; Wojciech PIEŃKOWSKI; Sylwia CIESIÓŁKA; Aneta KONWERSKA; Paul MOZDZIAK; Maciej BRĄZERT; Michal JEŠETA; Robert Z SPACZYŃSKI; Leszek PAWELCZYK a Bartosz KEMPISTY. Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation. Experimental and Molecular Pathology. SAN DIEGO: ELSEVIER, 2025, roč. 142, June 2025, s. 1-12. ISSN 0014-4800. Dostupné z: https://doi.org/10.1016/j.yexmp.2025.104965.

@article{2495138,
   author = {Kranc, Wiesława and Kaczmarek, Mariusz and Kowalska, Katarzyna and Pieńkowski, Wojciech and Ciesiółka, Sylwia and Konwerska, Aneta and Mozdziak, Paul and Brązert, Maciej and Ješeta, Michal and Spaczyński, Robert Z and Pawelczyk, Leszek and Kempisty, Bartosz},
   article_location = {SAN DIEGO},
   article_number = {June 2025},
   doi = {https://doi.org/10.1016/j.yexmp.2025.104965},
   keywords = {Human granulosa cells; Osteodifferentiation; Extracellular vesicles},
   language = {eng},
   issn = {0014-4800},
   journal = {Experimental and Molecular Pathology},
   title = {Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation},
   url = {https://www.sciencedirect.com/science/article/pii/S0014480025000140?via%3Dihub},
   volume = {142},
   year = {2025}
}

TY  - JOUR
ID  - 2495138
AU  - Kranc, Wiesława - Kaczmarek, Mariusz - Kowalska, Katarzyna - Pieńkowski, Wojciech - Ciesiółka, Sylwia - Konwerska, Aneta - Mozdziak, Paul - Brązert, Maciej - Ješeta, Michal - Spaczyński, Robert Z - Pawelczyk, Leszek - Kempisty, Bartosz
PY  - 2025
TI  - Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation
JF  - Experimental and Molecular Pathology
VL  - 142
IS  - June 2025
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER
SN  - 00144800
KW  - Human granulosa cells
KW  - Osteodifferentiation
KW  - Extracellular vesicles
UR  - https://www.sciencedirect.com/science/article/pii/S0014480025000140?via%3Dihub
N2  - Extracellular vesicles can play an important role in the processes occurring after stem cell transplantation, preventing cell apoptosis, stimulating immunological processes, and promoting the synthesis of extracellular matrix. Human follicular fluid (FF) can be a source of a subpopulation of cells with mesenchymal stem cells (MSCs) properties. Moreover these subpopulations of FF cells can differentiate into osteoblasts. In presented studies flow cytometry of ovarian FF cells confirmed positive expression of MSCs markers such as: CD44, CD90, CD105, CD73 and negative expression of a hematopoietic marker: CD45. The CD90+, CD105+, CD45- cell subpopulation has been obtained during magnetic separation using appropriate antibodies conjugated with microbeads. The extracellular vesicles (EVs) secreted by the cells during osteodifferentiation process differed from those secreted by cells culture in the basal medium. Based on the previous and current electron microscopy research, changes in size, number, and shape would support the notion that released EVs could be crucial to the ovarian FF cell subpopulation differentiation process. Osteogenic differentiation has been confirmed via Alizarin red staining. Therefore, follicular fluid (FF) can be a new source of a cell subpopulation with MSC properties, with the cells capable of differentiating into the osteogenic lineage. EVs could play a key role as mediators in tissue regeneration, especially bone tissue regeneration.
ER  -

KRANC, Wiesława; Mariusz KACZMAREK; Katarzyna KOWALSKA; Wojciech PIE$\backslash$'NKOWSKI; Sylwia CIESIÓŁKA; Aneta KONWERSKA; Paul MOZDZIAK; Maciej BRĄZERT; Michal JEŠETA; Robert Z SPACZY$\backslash$'NSKI; Leszek PAWELCZYK a Bartosz KEMPISTY. Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation. \textit{Experimental and Molecular Pathology}. SAN DIEGO: ELSEVIER, 2025, roč.~142, June 2025, s.~1-12. ISSN~0014-4800. Dostupné z: https://doi.org/10.1016/j.yexmp.2025.104965.

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