HPLC method for novel cannabinoid separation with
electrochemical detection on boron-doped diamond electrode

J 2025

HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode

BELBASI, Zeynab; Jan PETR; Jan HRBÁČ a David JIROVSKY

Základní údaje

Originální název

HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode

Autoři

BELBASI, Zeynab (203 Česká republika); Jan PETR (203 Česká republika); Jan HRBÁČ (203 Česká republika, garant, domácí) a David JIROVSKY (203 Česká republika)

Vydání

Journal of Pharmaceutical and Biomedical Analysis, Amsterdam, Elsevier Science BV, 2025, 0731-7085

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.100 v roce 2023

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jpba.2025.116875

UT WoS

001469844200001

EID Scopus

2-s2.0-105002246574

Klíčová slova anglicky

Cannabinoids; THC; HHC; CBD; HPLC; Electrochemical detection; Boron doped diamond

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 7. 2025 09:31, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this study, we developed a novel and environmentally friendly method for the determination of selected cannabinoids -cannabidiol, cannabinol, tetrahydrocannabinol, JWH 073 synthetic cannabinoid, hexahydrocannabinol (HHC), and hexahydrocannabinol-O-acetate - using high-performance liquid chromatography with electrochemical detection. The method employs a boron-doped diamond working electrode, which offers significant advantages in comparison with commonly used materials. Its main strengths include low background currents, reduced fouling, and an extended potential window, making it suitable for detecting compounds that are not readily oxidizable. The chromatographic system utilized a reversed-phase Gemini C18 column with a mobile phase consisting of acetonitrile and 25 mM phosphate buffer (pH 4.4) in step-gradient mode from 68 % to 88 % acetonitrile. The separation and determination of cannabinoids were optimized to be completed in less than 25 min. Hydrodynamic voltammetry was performed to identify the optimal potential for the amperometric detection, with a working potential of + 1400 mV (vs. Pd/H2) providing the best analytical response based on S/ N ratio. Under these optimal separation and detection conditions, the limits of detection were within the range of 6.1-666 nM (for cannabidiol and 9(R)-hexahydrocannabinol-O-acetate), while the response was linear within the concentration range measured up to 10 mu M. The method demonstrated good linearity, precision, and sensitivity, with limits of detection and quantitation reaching nanomolar levels. The developed method was successfully applied to commercial samples of HHC jelly bears and HHC distillate.

Citovat

BELBASI, Zeynab; Jan PETR; Jan HRBÁČ a David JIROVSKY. HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode. Journal of Pharmaceutical and Biomedical Analysis. Amsterdam: Elsevier Science BV, 2025, roč. 262, September, s. 116875-116880. ISSN 0731-7085. Dostupné z: https://dx.doi.org/10.1016/j.jpba.2025.116875.

@article{2495279,
   author = {Belbasi, Zeynab and Petr, Jan and Hrbáč, Jan and Jirovsky, David},
   article_location = {Amsterdam},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.jpba.2025.116875},
   keywords = {Cannabinoids; THC; HHC; CBD; HPLC; Electrochemical detection; Boron doped diamond},
   language = {eng},
   issn = {0731-7085},
   journal = {Journal of Pharmaceutical and Biomedical Analysis},
   title = {HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode},
   url = {https://www.sciencedirect.com/science/article/pii/S073170852500216X?via%3Dihub},
   volume = {262},
   year = {2025}
}

TY  - JOUR
ID  - 2495279
AU  - Belbasi, Zeynab - Petr, Jan - Hrbáč, Jan - Jirovsky, David
PY  - 2025
TI  - HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode
JF  - Journal of Pharmaceutical and Biomedical Analysis
VL  - 262
IS  - September
SP  - 116875
EP  - 116875
PB  - Elsevier Science BV
SN  - 07317085
KW  - Cannabinoids
KW  - THC
KW  - HHC
KW  - CBD
KW  - HPLC
KW  - Electrochemical detection
KW  - Boron doped diamond
UR  - https://www.sciencedirect.com/science/article/pii/S073170852500216X?via%3Dihub
N2  - In this study, we developed a novel and environmentally friendly method for the determination of selected cannabinoids -cannabidiol, cannabinol, tetrahydrocannabinol, JWH 073 synthetic cannabinoid, hexahydrocannabinol (HHC), and hexahydrocannabinol-O-acetate - using high-performance liquid chromatography with electrochemical detection. The method employs a boron-doped diamond working electrode, which offers significant advantages in comparison with commonly used materials. Its main strengths include low background currents, reduced fouling, and an extended potential window, making it suitable for detecting compounds that are not readily oxidizable. The chromatographic system utilized a reversed-phase Gemini C18 column with a mobile phase consisting of acetonitrile and 25 mM phosphate buffer (pH 4.4) in step-gradient mode from 68 % to 88 % acetonitrile. The separation and determination of cannabinoids were optimized to be completed in less than 25 min. Hydrodynamic voltammetry was performed to identify the optimal potential for the amperometric detection, with a working potential of + 1400 mV (vs. Pd/H2) providing the best analytical response based on S/ N ratio. Under these optimal separation and detection conditions, the limits of detection were within the range of 6.1-666 nM (for cannabidiol and 9(R)-hexahydrocannabinol-O-acetate), while the response was linear within the concentration range measured up to 10 mu M. The method demonstrated good linearity, precision, and sensitivity, with limits of detection and quantitation reaching nanomolar levels. The developed method was successfully applied to commercial samples of HHC jelly bears and HHC distillate.
ER  -

BELBASI, Zeynab; Jan PETR; Jan HRBÁČ a David JIROVSKY. HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode. \textit{Journal of Pharmaceutical and Biomedical Analysis}. Amsterdam: Elsevier Science BV, 2025, roč.~262, September, s.~116875-116880. ISSN~0731-7085. Dostupné z: https://dx.doi.org/10.1016/j.jpba.2025.116875.

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