Settling in: how entomopathogenic nematodes establish infection
at the molecular level

a 2025

Settling in: how entomopathogenic nematodes establish infection at the molecular level

ILGOVÁ, Jana; Sara ŠREIBR; Jiří VOREL; Pavel DOBEŠ; Martin KAŠNÝ et al.

Základní údaje

Originální název

Settling in: how entomopathogenic nematodes establish infection at the molecular level

Autoři

ILGOVÁ, Jana; Sara ŠREIBR; Jiří VOREL; Pavel DOBEŠ; Martin KAŠNÝ a Pavel HYRŠL

Vydání

29th Helminthological days, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00141162

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

entomopathogenic nematodes; transcriptomics; early infection phase; peptidases; inhibitors

Štítky

14314010, 14314020

Změněno: 8. 4. 2026 14:44, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Entomopathogenic nematodes (EPNs) are insect parasites that are capable of killing their hosts. Virulence of EPNs is boosted by symbiotic bacteria which cause fatal septicaemia in the insects. Unlike most parasitic nematodes that harm humans, animals, or crops, EPNs are seen as beneficial because they offer an eco-friendly alternative to chemical pesticides in pest control. Our research explores the molecular strategies employed by Heterorhabditis bacteriophora during the early stages of infection. This species invades insect hosts through active penetration by infective juveniles (IJs), the third-stage larvae specialised for host entry. Using Galleria mellonella (greater wax moth) larvae as a model, we conducted RNA-seq analysis across five critical time points (3, 6, 9, 12, and 15 hours post-infection) to capture the dynamic gene expression changes during infection. To overcome the challenge of limited IJ retrieval from infected wax moth larvae, we applied a single-cell RNA library preparation protocol, followed by high-throughput sequencing on the DNBSEQ-G400 (MGI) platform with the FCL PE100 kit. Differential expression analysis revealed key transcripts, with peptidases and their inhibitors showing some of the highest levels of regulation. Given the pivotal role of proteolysis in modulating insect immune responses, our findings suggest that these proteins may play a crucial role in immunosuppression during the establishment of infection.

Návaznosti

GA23-06457S, projekt VaV

Název: Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi

Investor: Grantová agentura ČR, Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

ILGOVÁ, Jana; Sara ŠREIBR; Jiří VOREL; Pavel DOBEŠ; Martin KAŠNÝ a Pavel HYRŠL. Settling in: how entomopathogenic nematodes establish infection at the molecular level. In 29th Helminthological days. 2025.

@proceedings{2496448,
   author = {Ilgová, Jana and Šreibr, Sara and Vorel, Jiří and Dobeš, Pavel and Kašný, Martin and Hyršl, Pavel},
   booktitle = {29th Helminthological days},
   keywords = {entomopathogenic nematodes; transcriptomics; early infection phase; peptidases; inhibitors},
   language = {eng},
   title = {Settling in: how entomopathogenic nematodes establish infection at the molecular level},
   year = {2025}
}

TY  - CONF
ID  - 2496448
AU  - Ilgová, Jana - Šreibr, Sara - Vorel, Jiří - Dobeš, Pavel - Kašný, Martin - Hyršl, Pavel
PY  - 2025
TI  - Settling in: how entomopathogenic nematodes establish infection at the molecular level
KW  - entomopathogenic nematodes
KW  - transcriptomics
KW  - early infection phase
KW  - peptidases
KW  - inhibitors
N2  - Entomopathogenic nematodes (EPNs) are insect parasites that are capable of killing their hosts. Virulence of EPNs is boosted by symbiotic bacteria which cause fatal septicaemia in the insects. Unlike most parasitic nematodes that harm humans, animals, or crops, EPNs are seen as beneficial because they offer an eco-friendly alternative to chemical pesticides in pest control. Our research explores the molecular strategies employed by Heterorhabditis bacteriophora during the early stages of infection. This species invades insect hosts through active penetration by infective juveniles (IJs), the third-stage larvae specialised for host entry. Using Galleria mellonella (greater wax moth) larvae as a model, we conducted RNA-seq analysis across five critical time points (3, 6, 9, 12, and 15 hours post-infection) to capture the dynamic gene expression changes during infection. To overcome the challenge of limited IJ retrieval from infected wax moth larvae, we applied a single-cell RNA library preparation protocol, followed by high-throughput sequencing on the DNBSEQ-G400 (MGI) platform with the FCL PE100 kit. Differential expression analysis revealed key transcripts, with peptidases and their inhibitors showing some of the highest levels of regulation. Given the pivotal role of proteolysis in modulating insect immune responses, our findings suggest that these proteins may play a crucial role in immunosuppression during the establishment of infection.
ER  -

ILGOVÁ, Jana; Sara ŠREIBR; Jiří VOREL; Pavel DOBEŠ; Martin KAŠNÝ a Pavel HYRŠL. Settling in: how entomopathogenic nematodes establish infection at the molecular level. In \textit{29th Helminthological days}. 2025.

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