Molecular Surveillance of Gastrointestinal Helminths in Wild
Ruminants: A Nationwide Study in the Czech Republic

a 2025

Molecular Surveillance of Gastrointestinal Helminths in Wild Ruminants: A Nationwide Study in the Czech Republic

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Jaroslav VADLEJCH a Radim PLHAL

Základní údaje

Originální název

Molecular Surveillance of Gastrointestinal Helminths in Wild Ruminants: A Nationwide Study in the Czech Republic

Autoři

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Jaroslav VADLEJCH a Radim PLHAL

Vydání

29th Helminthological days, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

40301 Veterinary science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Ashworthius sidemi; Haemonchus contortus; Fascioloides magna; rumen flukes; invasive parasites; wild ruminants; molekular screening

Příznaky

Mezinárodní význam

Změněno: 22. 5. 2025 13:45, Mgr. Nikol Reslová, Ph.D.

Anotace

V originále

Biological communities have undergone significant changes in recent years due to globalization, climate change, and increasing human socio-economic activities. These factors have facilitated the introduction of non-native species into new habitats, often accompanied by parasitic organisms known as "biological hitchhikers." Some of these parasites successfully adapt, reproduce, and spread in new environments, becoming invasive. This study focuses on two such invasive helminths: the haematophagous abomasal nematode Ashworthius sidemi and the giant liver fluke Fascioloides magna, with the primary objective of assessing their prevalence in wild ruminants across the Czech Republic and evaluating their potential ecological impact. A total of 983 fecal samples from red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama), and mouflon (Ovis musimon) were analyzed using multiplex real-time PCR assays targeting ribosomal DNA (rDNA) regions of six helminth taxa: A. sidemi, Haemonchus spp., F. magna, Dicrocoelium dendriticum, Fasciola hepatica, and Calicophoron daubneyi. The invasive nematode A. sidemi was the most prevalent species, detected in 15.8% of samples, with the highest infection rate in red deer (30.7%), while Haemonchus spp. were identified in 14.9% of samples, particularly in roe deer (23.2%). In contrast, trematode infections were less frequent, with F. magna and D. dendriticum each found in 1.5% of samples, and C. daubneyi in only 0.2%. Spatial analysis highlighted the extensive distribution of A. sidemi and Haemonchus spp. across nearly all regions. This study represents the first comprehensive molecular surveillance of helminths in wild ruminants across the Czech Republic, identifying potential infection hotspots and emphasizing the need for targeted health management strategies to mitigate the impacts of helminthiasis, particularly concerning invasive species.

Návaznosti

QL24010306, projekt VaV

Název: Udržitelné antiparazitární programy v chovech malých přežvýkavců odpovídající konceptu One Health

SS05010070, projekt VaV

Název: Šíření invazních druhů parazitů a jejich drtivé dopady na biologickou rozmanitost původních druhů přežvýkavců

Investor: Technologická agentura ČR, Šíření invazních druhů parazitů a jejich drtivé dopady na biologickou rozmanitost původních druhů přežvýkavců

Citovat

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Jaroslav VADLEJCH a Radim PLHAL. Molecular Surveillance of Gastrointestinal Helminths in Wild Ruminants: A Nationwide Study in the Czech Republic. In 29th Helminthological days. 2025.

@proceedings{2498097,
   author = {Reslová, Nikol and Škorpíková, Lucie and Vadlejch, Jaroslav and Plhal, Radim},
   booktitle = {29th Helminthological days},
   keywords = {Ashworthius sidemi; Haemonchus contortus; Fascioloides magna; rumen flukes; invasive parasites; wild ruminants; molekular screening},
   language = {eng},
   title = {Molecular Surveillance of Gastrointestinal Helminths in Wild Ruminants: A Nationwide Study in the Czech Republic},
   year = {2025}
}

TY  - CONF
ID  - 2498097
AU  - Reslová, Nikol - Škorpíková, Lucie - Vadlejch, Jaroslav - Plhal, Radim
PY  - 2025
TI  - Molecular Surveillance of Gastrointestinal Helminths in Wild Ruminants: A Nationwide Study in the Czech Republic
KW  - Ashworthius sidemi
KW  - Haemonchus contortus
KW  - Fascioloides magna
KW  - rumen flukes
KW  - invasive parasites
KW  - wild ruminants
KW  - molekular screening
N2  - Biological communities have undergone significant changes in recent years due to globalization, climate change, and increasing human socio-economic activities. These factors have facilitated the introduction of non-native species into new habitats, often accompanied by parasitic organisms known as "biological hitchhikers." Some of these parasites successfully adapt, reproduce, and spread in new environments, becoming invasive. This study focuses on two such invasive helminths: the haematophagous abomasal nematode Ashworthius sidemi and the giant liver fluke Fascioloides magna, with the primary objective of assessing their prevalence in wild ruminants across the Czech Republic and evaluating their potential ecological impact. A total of 983 fecal samples from red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama), and mouflon (Ovis musimon) were analyzed using multiplex real-time PCR assays targeting ribosomal DNA (rDNA) regions of six helminth taxa: A. sidemi, Haemonchus spp., F. magna, Dicrocoelium dendriticum, Fasciola hepatica, and Calicophoron daubneyi. The invasive nematode A. sidemi was the most prevalent species, detected in 15.8% of samples, with the highest infection rate in red deer (30.7%), while Haemonchus spp. were identified in 14.9% of samples, particularly in roe deer (23.2%). In contrast, trematode infections were less frequent, with F. magna and D. dendriticum each found in 1.5% of samples, and C. daubneyi in only 0.2%. Spatial analysis highlighted the extensive distribution of A. sidemi and Haemonchus spp. across nearly all regions. This study represents the first comprehensive molecular surveillance of helminths in wild ruminants across the Czech Republic, identifying potential infection hotspots and emphasizing the need for targeted health management strategies to mitigate the impacts of helminthiasis, particularly concerning invasive species.
ER  -

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