The Spatiotemporal Dynamics of Bone Remodeling During Physical
Activity and Reduced Mechanical Loading

a 2025

The Spatiotemporal Dynamics of Bone Remodeling During Physical Activity and Reduced Mechanical Loading

CIGOŠOVÁ, Klára; Margot ISSERTINE; Josef LAVICKÝ; Michaela KAVKOVÁ; Julian THEUIL et. al.

Základní údaje

Originální název

The Spatiotemporal Dynamics of Bone Remodeling During Physical Activity and Reduced Mechanical Loading

Autoři

CIGOŠOVÁ, Klára (203 Česká republika, garant, domácí); Margot ISSERTINE; Josef LAVICKÝ (203 Česká republika); Michaela KAVKOVÁ (203 Česká republika); Julian THEUIL; Sabina CERULOVÁ (203 Česká republika); Nela GAJDUŠKOVÁ (203 Česká republika); Thomas BRIOCHE; Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika); Angèle CHOPARD; Marcos GONZÁLEZ LÓPEZ (724 Španělsko) a Jan KŘIVÁNEK (203 Česká republika)

Vydání

American Association for Anatomy's Annual Meeting 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10605 Developmental biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/dvdy.70043

Klíčová slova anglicky

bone remodeling; mechanical unloading; bone morphology; physical activity

Změněno: 10. 7. 2025 14:17, Mgr. Tereza Slaměníková

Anotace

V originále

Bone remodeling is a continuous process that maintains bone strength and function through osteoclast-mediated bone resorption, followed by osteoblast-driven matrix deposition and mineralization. This sequence of cellular events is highly influenced by mechanical stimuli. Consequently, reduced mechanical load—such as during injury, prolonged bed rest, ageing, or spaceflight—can disrupt this balance, leading to decreased bone quality.While the role of mechanical stimuli and its relation to bone remodeling are extensively studied, the time-dependent dynamics of bone remodeling in relation to physical training and reduced mechanical loading remain completely unexplored. In this work, we investigate how reduced mechanical load and physical activity affect bone properties, morphology, and remodeling dynamics. To achieve this, we have designed a complex experimental setup followed by a combination of state-of-the-art techniques. Adult female Wistar rats underwent endurance(weight-bearing) and resistance (treadmill) training, followed by Hindlimb Unloading and enhanced Partial Weight Bearing to mimic reduced mechanical load and in parallel, spaceflight conditions. In vivo, assessments included performance monitoring during physical training and body composition analysis (EchoMRI). Subsequently, we took advantage of newly developed approachBEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) which enabled to evaluate remodeling dynamics of femur, humerus, and calvaria. Moreover, to refine the bone mechanical properties and morphology of different experimental conditions, micro-computed tomography was performed. Our results demonstrate that physical training before mechanical unloading preserves bone density, morphology, and function and as a first once precisely quantify these events. Furthermore, we show that mechanical unloading alone leads to a pronounced increase in bone resorption. Rats subjected to training exhibited enhanced load-carrying capacity, bearing up to three times their body weight. These findings underscore the critical role of physical training in mitigating the effects of reduced mechanical load and reveal, for the first time, the spatiotemporal dynamics of bone remodeling during this process. Our complex approach provides valuable insights for various clinical applications and future space missions, ensuring crew health.

Návaznosti

GA23-06160S, projekt VaV

Název: Fluktuace mikroprostředí kmenových buněk jako zdroj tkáňové adaptability ve zdraví a nemoci

Investor: Grantová agentura ČR, Fluktuace mikroprostředí kmenových buněk jako zdroj tkáňové adaptability ve zdraví a nemoci

Citovat

CIGOŠOVÁ, Klára; Margot ISSERTINE; Josef LAVICKÝ; Michaela KAVKOVÁ; Julian THEUIL; Sabina CERULOVÁ; Nela GAJDUŠKOVÁ; Thomas BRIOCHE; Petra BOŘILOVÁ LINHARTOVÁ; Angèle CHOPARD; Marcos GONZÁLEZ LÓPEZ a Jan KŘIVÁNEK. The Spatiotemporal Dynamics of Bone Remodeling During Physical Activity and Reduced Mechanical Loading. In American Association for Anatomy's Annual Meeting 2025. 2025. Dostupné z: https://dx.doi.org/10.1002/dvdy.70043.

@proceedings{2508020,
   author = {Cigošová, Klára and Issertine, Margot and Lavický, Josef and Kavková, Michaela and Theuil, Julian and Cerulová, Sabina and Gajdušková, Nela and Brioche, Thomas and Bořilová Linhartová, Petra and Chopard, Angèle and González López, Marcos and Křivánek, Jan},
   booktitle = {American Association for Anatomy's Annual Meeting 2025},
   doi = {http://dx.doi.org/10.1002/dvdy.70043},
   keywords = {bone remodeling; mechanical unloading; bone morphology; physical activity},
   language = {eng},
   title = {The Spatiotemporal Dynamics of Bone Remodeling During Physical Activity and Reduced Mechanical Loading},
   url = {https://anatomypubs.onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70043?utm_campaign=newsletter&utm_medium=email&utm_source=rasa_io},
   year = {2025}
}

TY  - CONF
ID  - 2508020
AU  - Cigošová, Klára - Issertine, Margot - Lavický, Josef - Kavková, Michaela - Theuil, Julian - Cerulová, Sabina - Gajdušková, Nela - Brioche, Thomas - Bořilová Linhartová, Petra - Chopard, Angèle - González López, Marcos - Křivánek, Jan
PY  - 2025
TI  - The Spatiotemporal Dynamics of Bone Remodeling During Physical Activity and Reduced Mechanical Loading
KW  - bone remodeling
KW  - mechanical unloading
KW  - bone morphology
KW  - physical activity
UR  - https://anatomypubs.onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70043?utm_campaign=newsletter&utm_medium=email&utm_source=rasa_io
N2  - Bone remodeling is a continuous process that maintains bone strength and function through osteoclast-mediated bone resorption, followed by osteoblast-driven matrix deposition and mineralization. This sequence of cellular events is highly influenced by mechanical stimuli. Consequently, reduced mechanical load—such as during injury, prolonged bed rest, ageing, or spaceflight—can disrupt this balance, leading to decreased bone quality.While the role of mechanical stimuli and its relation to bone remodeling are extensively studied, the time-dependent dynamics of bone remodeling in relation to physical training and reduced mechanical loading remain completely unexplored. In this work, we investigate how reduced mechanical load and physical activity affect bone properties, morphology, and remodeling dynamics. To achieve this, we have designed a complex experimental setup followed by a combination of state-of-the-art techniques. Adult female Wistar rats underwent endurance(weight-bearing) and resistance (treadmill) training, followed by Hindlimb Unloading and enhanced Partial Weight Bearing to mimic reduced mechanical load and in parallel, spaceflight conditions. In vivo, assessments included performance monitoring during physical training and body composition analysis (EchoMRI). Subsequently, we took advantage of newly developed approachBEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) which enabled to evaluate remodeling dynamics of femur, humerus, and calvaria. Moreover, to refine the bone mechanical properties and morphology of different experimental conditions, micro-computed tomography was performed. Our results demonstrate that physical training before mechanical unloading preserves bone density, morphology, and function and as a first once precisely quantify these events. Furthermore, we show that mechanical unloading alone leads to a pronounced increase in bone resorption. Rats subjected to training exhibited enhanced load-carrying capacity, bearing up to three times their body weight. These findings underscore the critical role of physical training in mitigating the effects of reduced mechanical load and reveal, for the first time, the spatiotemporal dynamics of bone remodeling during this process. Our complex approach provides valuable insights for various clinical applications and future space missions, ensuring crew health.
ER  -

CIGOŠOVÁ, Klára; Margot ISSERTINE; Josef LAVICKÝ; Michaela KAVKOVÁ; Julian THEUIL; Sabina CERULOVÁ; Nela GAJDUŠKOVÁ; Thomas BRIOCHE; Petra BOŘILOVÁ LINHARTOVÁ; Angèle CHOPARD; Marcos GONZÁLEZ LÓPEZ a Jan KŘIVÁNEK. The Spatiotemporal Dynamics of Bone Remodeling During Physical Activity and Reduced Mechanical Loading. In \textit{American Association for Anatomy's Annual Meeting 2025}. 2025. Dostupné z: https://dx.doi.org/10.1002/dvdy.70043.

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