Haloalkane dehalogenases other than LinB can contribute to the
γ-hexachlorocyclohexane utilization

J 2025

Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization

CHEN, Nannan; Kouhei KISHIDA; Leonardo STARI; Ryota MORIUCHI; Yoshiyuki OHTSUBO et al.

Základní údaje

Originální název

Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization

Autoři

CHEN, Nannan; Kouhei KISHIDA; Leonardo STARI; Ryota MORIUCHI; Yoshiyuki OHTSUBO; Jiří DAMBORSKÝ a Yuji NAGATA

Vydání

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, ENGLAND, OXFORD UNIV PRESS, 2025, 0916-8451

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.300 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00141583

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

haloalkane dehalogenase; xenobiotics; pesticide; gamma-hexachlorocyclohexane; sphingomonads

Štítky

14314010, 143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 2. 2026 08:14, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of gamma-hexachlorocyclohexane (gamma-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward gamma-HCH utilization, the linB gene in gamma-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from gamma-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with gamma-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the gamma-HCH utilization.

Návaznosti

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90269, velká výzkumná infrastruktura

Název: RECETOX RI II

Citovat

CHEN, Nannan; Kouhei KISHIDA; Leonardo STARI; Ryota MORIUCHI; Yoshiyuki OHTSUBO; Jiří DAMBORSKÝ a Yuji NAGATA. Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. ENGLAND: OXFORD UNIV PRESS, 2025, roč. 89, č. 6, s. 898-909. ISSN 0916-8451. Dostupné z: https://doi.org/10.1093/bbb/zbaf022.

@article{2508038,
   author = {Chen, Nannan and Kishida, Kouhei and Stari, Leonardo and Moriuchi, Ryota and Ohtsubo, Yoshiyuki and Damborský, Jiří and Nagata, Yuji},
   article_location = {ENGLAND},
   article_number = {6},
   doi = {https://doi.org/10.1093/bbb/zbaf022},
   keywords = {haloalkane dehalogenase; xenobiotics; pesticide; gamma-hexachlorocyclohexane; sphingomonads},
   language = {eng},
   issn = {0916-8451},
   journal = {BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY},
   note = {Nedoplňovat EF17_043/0009632.},
   title = {Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization},
   url = {https://academic.oup.com/bbb/article-abstract/89/6/898/8052007?redirectedFrom=fulltext&login=false},
   volume = {89},
   year = {2025}
}

TY  - JOUR
ID  - 2508038
AU  - Chen, Nannan - Kishida, Kouhei - Stari, Leonardo - Moriuchi, Ryota - Ohtsubo, Yoshiyuki - Damborský, Jiří - Nagata, Yuji
PY  - 2025
TI  - Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization
JF  - BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
VL  - 89
IS  - 6
SP  - 898-909
EP  - 898-909
PB  - OXFORD UNIV PRESS
SN  - 09168451
N1  - Nedoplňovat EF17_043/0009632.
KW  - haloalkane dehalogenase
KW  - xenobiotics
KW  - pesticide
KW  - gamma-hexachlorocyclohexane
KW  - sphingomonads
UR  - https://academic.oup.com/bbb/article-abstract/89/6/898/8052007?redirectedFrom=fulltext&login=false
N2  - Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of gamma-hexachlorocyclohexane (gamma-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward gamma-HCH utilization, the linB gene in gamma-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from gamma-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with gamma-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the gamma-HCH utilization.
ER  -

CHEN, Nannan; Kouhei KISHIDA; Leonardo STARI; Ryota MORIUCHI; Yoshiyuki OHTSUBO; Jiří DAMBORSKÝ a Yuji NAGATA. Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization. \textit{BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY}. ENGLAND: OXFORD UNIV PRESS, 2025, roč.~89, č.~6, s.~898-909. ISSN~0916-8451. Dostupné z: https://doi.org/10.1093/bbb/zbaf022.

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