Transcriptional and phenotypical alterations associated with a
gradual benzo[a]pyrene-induced transition of human bronchial
epithelial cells into mesenchymal-like cells

J 2024

Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells

HYZDALOVA, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVA; Katerina PENCIKOVA; Simona STRAPÁČOVÁ et al.

Základní údaje

Originální název

Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells

Autoři

Vydání

Environmental Toxicology and Pharmacology, Amsterdam, Elsevier Science B.V. 2024, 1382-6689

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.200

Označené pro přenos do RIV

DOI

https://doi.org/10.1016/j.etap.2024.104424

UT WoS

001215550700001

Klíčová slova anglicky

Benzo[a]pyrene; Aryl hydrocarbon receptor; 2,3,7,8-tetrachlorodibenzo-p-dioxin; Epithelial-mesenchymal transition; Human bronchial epithelial cells

Štítky

RIV ne

Změněno: 28. 7. 2025 17:10, Mgr. Jiřina Procházková, Ph.D.

Anotace

V originále

The role of benzo[a]pyrene (BaP), a prominent genotoxic carcinogen and aryl hydrocarbon receptor (AhR) ligand, in tumor progression remains poorly characterized. We investigated the impact of BaP on the process of epithelial-mesenchymal transition (EMT) in normal human bronchial epithelial HBEC-12KT cells. Early morphological changes after 2-week exposure were accompanied with induction of SERPINB2, IL1, CDKN1A/ p21 (linked with cell cycle delay) and chemokine CXCL5. After 8-week exposure, induction of cell migration and EMT-related pattern of markers/regulators led to induction of further pro-inflammatory cytokines or noncanonical Wnt pathway ligand WNT5A. This trend of up-regulation of pro-inflammatory genes and noncanonical Wnt pathway constituents was observed also in the BaP-transformed HBEC-12KT-B1 cells. In general, transcriptional effects of BaP differed from those of TGF beta 1, a prototypical EMT inducer, or a model nongenotoxic AhR ligand, TCDD. Carcinogenic polycyclic aromatic hydrocarbons could thus induce a unique set of molecular changes linked with EMT and cancer progression.

Citovat

HYZDALOVA, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVA; Katerina PENCIKOVA; Simona STRAPÁČOVÁ; Jana SLOVÁČKOVÁ; Simona KAJABOVA; Helena LIBALOVA; Jan TOPINKA; Markéta KABÁTKOVÁ; Jan VONDRÁČEK; Steen MOLLERUP a Miroslav MACHALA. Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells. Environmental Toxicology and Pharmacology. Amsterdam: Elsevier Science B.V., 2024, roč. 107, 13 s. ISSN 1382-6689. Dostupné z: https://doi.org/10.1016/j.etap.2024.104424.

@article{2510238,
   author = {Hyzdalova, Martina and Procházková, Jiřina and Strakova, Nicol and Pencikova, Katerina and Strapáčová, Simona and Slováčková, Jana and Kajabova, Simona and Libalova, Helena and Topinka, Jan and Kabátková, Markéta and Vondráček, Jan and Mollerup, Steen and Machala, Miroslav},
   article_location = {Amsterdam},
   doi = {https://doi.org/10.1016/j.etap.2024.104424},
   keywords = {Benzo[a]pyrene; Aryl hydrocarbon receptor; 2,3,7,8-tetrachlorodibenzo-p-dioxin; Epithelial-mesenchymal transition; Human bronchial epithelial cells},
   issn = {1382-6689},
   journal = {Environmental Toxicology and Pharmacology},
   title = {Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells},
   volume = {107},
   year = {2024}
}

TY  - JOUR
ID  - 2510238
AU  - Hyzdalova, Martina - Procházková, Jiřina - Strakova, Nicol - Pencikova, Katerina - Strapáčová, Simona - Slováčková, Jana - Kajabova, Simona - Libalova, Helena - Topinka, Jan - Kabátková, Markéta - Vondráček, Jan - Mollerup, Steen - Machala, Miroslav
PY  - 2024
TI  - Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells
JF  - Environmental Toxicology and Pharmacology
VL  - 107
PB  - Elsevier Science B.V.
SN  - 13826689
KW  - Benzo[a]pyrene
KW  - Aryl hydrocarbon receptor
KW  - 2,3,7,8-tetrachlorodibenzo-p-dioxin
KW  - Epithelial-mesenchymal transition
KW  - Human bronchial epithelial cells
N2  - The role of benzo[a]pyrene (BaP), a prominent genotoxic carcinogen and aryl hydrocarbon receptor (AhR) ligand, in tumor progression remains poorly characterized. We investigated the impact of BaP on the process of epithelial-mesenchymal transition (EMT) in normal human bronchial epithelial HBEC-12KT cells. Early morphological changes after 2-week exposure were accompanied with induction of SERPINB2, IL1, CDKN1A/ p21 (linked with cell cycle delay) and chemokine CXCL5. After 8-week exposure, induction of cell migration and EMT-related pattern of markers/regulators led to induction of further pro-inflammatory cytokines or noncanonical Wnt pathway ligand WNT5A. This trend of up-regulation of pro-inflammatory genes and noncanonical Wnt pathway constituents was observed also in the BaP-transformed HBEC-12KT-B1 cells. In general, transcriptional effects of BaP differed from those of TGF beta 1, a prototypical EMT inducer, or a model nongenotoxic AhR ligand, TCDD. Carcinogenic polycyclic aromatic hydrocarbons could thus induce a unique set of molecular changes linked with EMT and cancer progression.
ER  -

HYZDALOVA, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVA; Katerina PENCIKOVA; Simona STRAPÁČOVÁ; Jana SLOVÁČKOVÁ; Simona KAJABOVA; Helena LIBALOVA; Jan TOPINKA; Markéta KABÁTKOVÁ; Jan VONDRÁČEK; Steen MOLLERUP a Miroslav MACHALA. Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells. \textit{Environmental Toxicology and Pharmacology}. Amsterdam: Elsevier Science B.V., 2024, roč.~107, 13 s. ISSN~1382-6689. Dostupné z: https://doi.org/10.1016/j.etap.2024.104424.

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