Thermoresponsive Hyaluronate-Based Nanogels for Enhanced
Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer

J 2025

Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer

LATECKA, Filip; Tamara JURIŇÁKOVÁ; Lukas MUNSTER; Monika MUCHOVA; Michal MASAŘÍK et al.

Základní údaje

Originální název

Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer

Autoři

LATECKA, Filip; Tamara JURIŇÁKOVÁ ; Lukas MUNSTER; Monika MUCHOVA; Michal MASAŘÍK; Anton KUCHYNSKI; Petr HUMPOLICEK; Michaela FOJTŮ a Jan VICHA

Vydání

Biomacromolecules, Washington, American Chemical Society, 2025, 1525-7797

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.400 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

hyaluronic acid nanogels; pNIPAM; phenanthriplatin delivery; thermoresponsive carriers; ovarian cancer therapy

Štítky

14110515, 14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 2. 2026 09:51, Mgr. Eva Suchánková

Anotace

V originále

Stimuli-responsive hyaluronic acid carriers face limitations due to limited carboxyl groups, which are divided between drug conjugation and functional modifications. Thermoresponsive nanogels based on selectively oxidized hyaluronan (2,3-dicarboxy hyaluronate, DCH) grafted with poly(N-isopropyl acrylamide) (pNIPAM) were developed for phenanthriplatin (PhPt) delivery. Sequential oxidation after pNIPAM grafting introduced additional carboxylic groups, enabling a more efficient drug loading and controlled release. Compared to nonoxidized pNIPAM-modified HA, this approach achieved 3 times higher loading efficacy and significantly slower drug release. Upon PhPt loading, DCH-pNIPAM conjugates self-assembled into nanogels, with the drug binding mode (ionic vs covalent) influencing particle rearrangement and drug release behavior. Covalently bound PhPt showed reduced release compared to nonthermoresponsive controls. In vitro studies on ovarian cancer cell lines, including cisplatin-resistant variants, demonstrated up to an 18-fold increase in cytotoxicity versus free PhPt. These nanogels offer a promising strategy for enhancing drug efficacy, reducing off-target effects, and overcoming resistance in cancer therapy.

Návaznosti

MUNI/A/1587/2023, interní kód MU

Název: Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň

Investor: Masarykova univerzita, Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň

MUNI/A/1790/2024, interní kód MU

Název: Patofyziologie běžných komplexních nemocí: od molekuly k organizmu

Investor: Masarykova univerzita, Patofyziologie běžných komplexních nemocí: od molekuly k organizmu

NW24-03-00331, projekt VaV

Název: Selektivně modifikované betaglukany jako imunoaktivní nosiče protinádorových léčiv pro léčbu nádorů tlustého střeva

Investor: Ministerstvo zdravotnictví ČR, Selektivně modifikované betaglukany jako imunoaktivní nosiče protinádorových léčiv pro léčbu nádorů tlustého střeva, Podprogram 1 - standardní

Citovat

LATECKA, Filip; Tamara JURIŇÁKOVÁ; Lukas MUNSTER; Monika MUCHOVA; Michal MASAŘÍK; Anton KUCHYNSKI; Petr HUMPOLICEK; Michaela FOJTŮ a Jan VICHA. Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer. Biomacromolecules. Washington: American Chemical Society, 2025, roč. 26, č. 8, s. 5232-5244. ISSN 1525-7797. Dostupné z: https://doi.org/10.1021/acs.biomac.5c00692.

@article{2511229,
   author = {Latecka, Filip and Juriňáková, Tamara and Munster, Lukas and Muchova, Monika and Masařík, Michal and Kuchynski, Anton and Humpolicek, Petr and Fojtů, Michaela and Vicha, Jan},
   article_location = {Washington},
   article_number = {8},
   doi = {https://doi.org/10.1021/acs.biomac.5c00692},
   keywords = {hyaluronic acid nanogels; pNIPAM; phenanthriplatin delivery; thermoresponsive carriers; ovarian cancer therapy},
   language = {eng},
   issn = {1525-7797},
   journal = {Biomacromolecules},
   title = {Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer},
   url = {https://pubs.acs.org/doi/10.1021/acs.biomac.5c00692},
   volume = {26},
   year = {2025}
}

TY  - JOUR
ID  - 2511229
AU  - Latecka, Filip - Juriňáková, Tamara - Munster, Lukas - Muchova, Monika - Masařík, Michal - Kuchynski, Anton - Humpolicek, Petr - Fojtů, Michaela - Vicha, Jan
PY  - 2025
TI  - Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer
JF  - Biomacromolecules
VL  - 26
IS  - 8
SP  - 5232-5244
EP  - 5232-5244
PB  - American Chemical Society
SN  - 15257797
KW  - hyaluronic acid nanogels
KW  - pNIPAM
KW  - phenanthriplatin delivery
KW  - thermoresponsive carriers
KW  - ovarian cancer therapy
UR  - https://pubs.acs.org/doi/10.1021/acs.biomac.5c00692
N2  - Stimuli-responsive hyaluronic acid carriers face limitations due to limited carboxyl groups, which are divided between drug conjugation and functional modifications. Thermoresponsive nanogels based on selectively oxidized hyaluronan (2,3-dicarboxy hyaluronate, DCH) grafted with poly(N-isopropyl acrylamide) (pNIPAM) were developed for phenanthriplatin (PhPt) delivery. Sequential oxidation after pNIPAM grafting introduced additional carboxylic groups, enabling a more efficient drug loading and controlled release. Compared to nonoxidized pNIPAM-modified HA, this approach achieved 3 times higher loading efficacy and significantly slower drug release. Upon PhPt loading, DCH-pNIPAM conjugates self-assembled into nanogels, with the drug binding mode (ionic vs covalent) influencing particle rearrangement and drug release behavior. Covalently bound PhPt showed reduced release compared to nonthermoresponsive controls. In vitro studies on ovarian cancer cell lines, including cisplatin-resistant variants, demonstrated up to an 18-fold increase in cytotoxicity versus free PhPt. These nanogels offer a promising strategy for enhancing drug efficacy, reducing off-target effects, and overcoming resistance in cancer therapy.
ER  -

LATECKA, Filip; Tamara JURIŇÁKOVÁ; Lukas MUNSTER; Monika MUCHOVA; Michal MASAŘÍK; Anton KUCHYNSKI; Petr HUMPOLICEK; Michaela FOJTŮ a Jan VICHA. Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer. \textit{Biomacromolecules}. Washington: American Chemical Society, 2025, roč.~26, č.~8, s.~5232-5244. ISSN~1525-7797. Dostupné z: https://doi.org/10.1021/acs.biomac.5c00692.

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