Embryonic and postnatal macrophages are essential for dental
morphogenesis and self-renewal

a 2025

Embryonic and postnatal macrophages are essential for dental morphogenesis and self-renewal

GONZÁLEZ LÓPEZ, Marcos; Kaitlin A. KATSURA; Vitor C.M. NEVES; Ruslan SOLDATOV; Josef LAVICKÝ et al.

Základní údaje

Originální název

Embryonic and postnatal macrophages are essential for dental morphogenesis and self-renewal

Autoři

Vydání

15th Tooth Morphogenesis & Differentiation Conference, 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10605 Developmental biology

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

macrophages; dental morphogenesis; tooth development; self-renewal; developmental biology

Příznaky

Mezinárodní význam

Změněno: 13. 4. 2026 15:03, Mgr. Tereza Miškechová

Anotace

V originále

The role of macrophages in teeth, beyond their function in innate immunity, remains unexplored. This study demonstrates that macrophages populate dental tissues during early development and increase in number during pre-eruptive stages postnatally. In continuously growing teeth, they are associated with epithelial and mesenchymal stem cell niches throughout lifetime. To investigate their role in development, we genetically disrupted macrophage migration using neural-crest specific Wnt1Cre/Csf1fl/fl and generalCsf1R knockout organisms. This resulted in abnormal dentin and enamel deposition, eruption defects, and early tooth mispatterning. Notably, the phenotype was partially rescued via bone marrow transplantation. In healthy adults, short-term pharmacological depletion of macrophages caused striking but temporary damage of dental tissues. Following treatment, macrophages rapidly repopulated dental tissue and polarized from M0 to M2 reparative state, leading to tissue restoration. Overall, our findings reveal essential role of macrophages in the dental development and patterning of both mesenchymal and epithelial tooth compartments. This non-immune role of dental macrophages is reminiscent of their function in complex tissue regeneration and requires future studies to dissect precise cellular and molecular mechanisms.

Návaznosti

GA25-18087S, projekt VaV

Název: Buněčná podstata hojení zubu

Investor: Grantová agentura ČR, Buněčná podstata hojení zubu

Citovat

GONZÁLEZ LÓPEZ, Marcos; Kaitlin A. KATSURA; Vitor C.M. NEVES; Ruslan SOLDATOV; Josef LAVICKÝ; Maryam AZAM; Michaela KAVKOVÁ; Klára CIGOŠOVÁ; Brian TEMSAMRIT; Hannah GONG; Kelsey NEMEC; Frederick Christian BENETT; Haneen Riadh Ali TUAIMA; Val YIANNI; Paul T. SHARPE; Marc BAJENOFF; Peter KHARCHENKO; Mariko L BENNETT; Igor ADAMEYKO a Jan KŘIVÁNEK. Embryonic and postnatal macrophages are essential for dental morphogenesis and self-renewal. In 15th Tooth Morphogenesis & Differentiation Conference, 2025. 2025.

@proceedings{2511230,
   author = {González López, Marcos and Katsura, Kaitlin A. and Neves, Vitor C.M. and Soldatov, Ruslan and Lavický, Josef and Azam, Maryam and Kavková, Michaela and Cigošová, Klára and Temsamrit, Brian and Gong, Hannah and Nemec, Kelsey and Benett, Frederick Christian and Tuaima, Haneen Riadh Ali and Yianni, Val and Sharpe, Paul T. and Bajenoff, Marc and Kharchenko, Peter and Bennett, Mariko L and Adameyko, Igor and Křivánek, Jan},
   booktitle = {15th Tooth Morphogenesis & Differentiation Conference, 2025},
   keywords = {macrophages; dental morphogenesis; tooth development; self-renewal; developmental biology},
   language = {eng},
   title = {Embryonic and postnatal macrophages are essential for dental morphogenesis and self-renewal},
   year = {2025}
}

TY  - CONF
ID  - 2511230
AU  - González López, Marcos - Katsura, Kaitlin A. - Neves, Vitor C.M. - Soldatov, Ruslan - Lavický, Josef - Azam, Maryam - Kavková, Michaela - Cigošová, Klára - Temsamrit, Brian - Gong, Hannah - Nemec, Kelsey - Benett, Frederick Christian - Tuaima, Haneen Riadh Ali - Yianni, Val - Sharpe, Paul T. - Bajenoff, Marc - Kharchenko, Peter - Bennett, Mariko L - Adameyko, Igor - Křivánek, Jan
PY  - 2025
TI  - Embryonic and postnatal macrophages are essential for dental morphogenesis and self-renewal
KW  - macrophages
KW  - dental morphogenesis
KW  - tooth development
KW  - self-renewal
KW  - developmental biology
N2  - The role of macrophages in teeth, beyond their function in innate immunity, remains unexplored. This study demonstrates that macrophages populate dental tissues during early development and increase in number during pre-eruptive stages postnatally. In continuously growing teeth, they are associated with epithelial and mesenchymal stem cell niches throughout lifetime. To investigate their role in development, we genetically disrupted macrophage migration using neural-crest specific Wnt1Cre/Csf1fl/fl and generalCsf1R knockout organisms. This resulted in abnormal dentin and enamel deposition, eruption defects, and early tooth mispatterning. Notably, the phenotype was partially rescued via bone marrow transplantation. In healthy adults, short-term pharmacological depletion of macrophages caused striking but temporary damage of dental tissues. Following treatment, macrophages rapidly repopulated dental tissue and polarized from M0 to M2 reparative state, leading to tissue restoration. Overall, our findings reveal essential role of macrophages in the dental development and patterning of both mesenchymal and epithelial tooth compartments. This non-immune role of dental macrophages is reminiscent of their function in complex tissue regeneration and requires future studies to dissect precise cellular and molecular mechanisms.
ER  -

GONZÁLEZ LÓPEZ, Marcos; Kaitlin A. KATSURA; Vitor C.M. NEVES; Ruslan SOLDATOV; Josef LAVICKÝ; Maryam AZAM; Michaela KAVKOVÁ; Klára CIGOŠOVÁ; Brian TEMSAMRIT; Hannah GONG; Kelsey NEMEC; Frederick Christian BENETT; Haneen Riadh Ali TUAIMA; Val YIANNI; Paul T. SHARPE; Marc BAJENOFF; Peter KHARCHENKO; Mariko L BENNETT; Igor ADAMEYKO a Jan KŘIVÁNEK. Embryonic and postnatal macrophages are essential for dental morphogenesis and self-renewal. In \textit{15th Tooth Morphogenesis \&{} Differentiation Conference, 2025}. 2025.

Přehled o publikaci