Prevalence and characterization of PVL-positive MRSA isolates
in Moravian region, Czech Republic (2021-2024)

k 2025

Prevalence and characterization of PVL-positive MRSA isolates in Moravian region, Czech Republic (2021-2024)

KARPÍŠKOVÁ, Renata; Ivana KOLÁČKOVÁ; Kristýna BRODÍKOVÁ a Kateřina FIŠEROVÁ

Základní údaje

Originální název

Prevalence and characterization of PVL-positive MRSA isolates in Moravian region, Czech Republic (2021-2024)

Autoři

KARPÍŠKOVÁ, Renata; Ivana KOLÁČKOVÁ; Kristýna BRODÍKOVÁ a Kateřina FIŠEROVÁ

Vydání

Congress of the European Society of  Clinical Microbiology and Infectious Diseases, 2025

Další údaje

Typ výsledku

Prezentace na konferencích

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 8. 2025 12:23, Mgr. Kristýna Brodíková, Ph.D.

Anotace

V originále

Background The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) varies significantly based on the region and specific environment, such as hospitals or community settings. Some MRSA isolates also produce Panton-Valentine leukocidin (PVL), a bicomponent pore-forming cytolytic toxin encoded by the lukF-PV and lukS-PV genes. The context of the study was to investigate the prevalence and characteristics of PVL-positive MRSA isolates in two Moravian regions of the Czech Republic. The study aims to understand the distribution of different spa types among these PVL-positive MRSA isolates, which can provide insights into the epidemiology and potential spread of these strains in both healthcare and community settings. Methods MRSA isolates were consecutively obtained from two clinical laboratories in two Moravian regions between 2021 and 2024. Both laboratories collected isolates from healthcare and community settings. Only unique-patient isolates for that period were included in the study. PCR was used to target the SA-442 species-specific fragment, the mecA/mecC, and the lukF/lukS-PV genes. Obtained isolates were characterized by spa and MLST typing. Results A total of 350 MRSA isolates were collected, 38 of which were positive for lukF/lukS-PV genes. Among the PVL-positive isolates, 13 different spa types were identified. The most frequent was t034 (13 times), followed by t437 (7 times), t003 (4 times), and t008 (4 times). Conclusions The two most frequent spa types, t034 and t437, detected in our study among inpatients and outpatients, belong to the predominant community-associated MRSA clones in Asia. Both spa types have increased in Europe during the past decade. While the “old” PVL-positive t034 mainly belonged to ST398, the more recent isolates in our study belonged to ST1232, emerging as a new successful sub-clade of MRSA. In the Czech Republic, traditional spa types t003 and t008 were found in a minority of PVL-positive MRSA isolates.

Návaznosti

MUNI/A/1545/2024, interní kód MU

Název: Prohlubování znalostí v oblasti zdravotních rizik a benefitů výživy, prostředí a životního stylu VII

Investor: Masarykova univerzita, Prohlubování znalostí v oblasti zdravotních rizik a benefitů výživy, prostředí a životního stylu VII

NU23-09-00488, projekt VaV

Název: Epidemiologická a genetická analýza meticilin-rezistentních kmenů Staphylococcus aureus dle konceptu WHO “One Health”

Investor: Ministerstvo zdravotnictví ČR, Epidemiologická a genetická analýza meticillin rezistentních kmenů Staphylococcus aureus dle konceptu WHO "One health", Podprogram 1 - standardní

Citovat

KARPÍŠKOVÁ, Renata; Ivana KOLÁČKOVÁ; Kristýna BRODÍKOVÁ a Kateřina FIŠEROVÁ. Prevalence and characterization of PVL-positive MRSA isolates in Moravian region, Czech Republic (2021-2024). In Congress of the European Society of  Clinical Microbiology and Infectious Diseases. 2025.

@proceedings{2511423,
   author = {Karpíšková, Renata and Koláčková, Ivana and Brodíková, Kristýna and Fišerová, Kateřina},
   booktitle = {Congress of the European Society of  Clinical Microbiology and Infectious Diseases},
   title = {Prevalence and characterization of PVL-positive MRSA isolates in Moravian region, Czech Republic (2021-2024)},
   year = {2025}
}

TY  - CONF
ID  - 2511423
AU  - Karpíšková, Renata - Koláčková, Ivana - Brodíková, Kristýna - Fišerová, Kateřina
PY  - 2025
TI  - Prevalence and characterization of PVL-positive MRSA isolates in Moravian region, Czech Republic (2021-2024)
N2  - Background The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) varies significantly based on the region and specific environment, such as hospitals or community settings. Some MRSA isolates also produce Panton-Valentine leukocidin (PVL), a bicomponent pore-forming cytolytic toxin encoded by the lukF-PV and lukS-PV genes. The context of the study was to investigate the prevalence and characteristics of PVL-positive MRSA isolates in two Moravian regions of the Czech Republic. The study aims to understand the distribution of different spa types among these PVL-positive MRSA isolates, which can provide insights into the epidemiology and potential spread of these strains in both healthcare and community settings. Methods MRSA isolates were consecutively obtained from two clinical laboratories in two Moravian regions between 2021 and 2024. Both laboratories collected isolates from healthcare and community settings. Only unique-patient isolates for that period were included in the study. PCR was used to target the SA-442 species-specific fragment, the mecA/mecC, and the lukF/lukS-PV genes. Obtained isolates were characterized by spa and MLST typing. Results A total of 350 MRSA isolates were collected, 38 of which were positive for lukF/lukS-PV genes. Among the PVL-positive isolates, 13 different spa types were identified. The most frequent was t034 (13 times), followed by t437 (7 times), t003 (4 times), and t008 (4 times). Conclusions The two most frequent spa types, t034 and t437, detected in our study among inpatients and outpatients, belong to the predominant community-associated MRSA clones in Asia. Both spa types have increased in Europe during the past decade. While the “old” PVL-positive t034 mainly belonged to ST398, the more recent isolates in our study belonged to ST1232, emerging as a new successful sub-clade of MRSA. In the Czech Republic, traditional spa types t003 and t008 were found in a minority of PVL-positive MRSA isolates.
ER  -

KARPÍŠKOVÁ, Renata; Ivana KOLÁČKOVÁ; Kristýna BRODÍKOVÁ a Kateřina FIŠEROVÁ. Prevalence and characterization of PVL-positive MRSA isolates in Moravian region, Czech Republic (2021-2024). In \textit{Congress of the European Society of  Clinical Microbiology and Infectious Diseases}. 2025.

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