Unraveling the Transcription Factor Code of Odontoblast
Differentiation

J 2026

Unraveling the Transcription Factor Code of Odontoblast Differentiation

LAVICKÝ, Josef; Marcos GONZÁLEZ LÓPEZ; Václav CHOCHOLA; Petra KOMPANÍKOVÁ; Vladislav RAKULTSEV et al.

Základní údaje

Originální název

Unraveling the Transcription Factor Code of Odontoblast Differentiation

Autoři

Vydání

Journal of Dental Research, Thousand Oaks, Sage Publication, 2026, 0022-0345

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.900 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

tooth development; odontogenesis; dentin; bioengineering; cell differentiation; stem cell(s)

Štítky

14110517, 14314010, CF CELLIM

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 3. 2026 11:43, Mgr. Tereza Miškechová

Anotace

V originále

The molecular mechanisms controlling the differentiation of stem cells into specialized cells and tissues are enormously complex. Deciphering the mechanisms behind this precisely regulated process is essential not only for understanding ontogenesis but also for interpretations of evolutionary dynamics. A deep understanding of differentiation processes in various cell types would open the way for safe and targeted tissue engineering. This was previously limited by technical constraints, but the recent development of multiomic approaches has overcome these barriers, providing unprecedented insight into the intricate workings of this molecular machinery. Based on single-cell RNA-seq analyses of continuously growing mouse teeth, 4 transcription factors (Etv4, Gsc, Sall1, and Nupr1) were selected. Their role during tooth development has not been previously evaluated. Here, we provide evidence about their function in the differentiation of odontoblasts-dental cells responsible for dentin production. Our results revealed that controlled overexpression of specific transcription factors directly in mouse-induced pluripotent stem cells is sufficient to guide their differentiation into cells with an odontoblast-like phenotype, both in vitro and in vivo. The differentiated cells exhibited upregulated expression of odontoblast-specific molecular markers (DMP1 and DSP) as well as production of collagenous and mineralized tissue. We demonstrate that a deep insight into fundamental developmental events can provide a powerful basis for innovative cell differentiation approaches. Taken together, this research might serve as a proof of concept for using large-omics data in the generation of specific, differentiated cell types by controlled expression of a particular transcription factor code. This may represent a turning point in both developmental biology and regenerative medicine fields.

Návaznosti

GA23-06160S, projekt VaV

Název: Fluktuace mikroprostředí kmenových buněk jako zdroj tkáňové adaptability ve zdraví a nemoci

Investor: Grantová agentura ČR, Fluktuace mikroprostředí kmenových buněk jako zdroj tkáňové adaptability ve zdraví a nemoci

GA25-17360S, projekt VaV

Název: Molekulární a buněčné principy embryonálního vývoje choroidního plexu

Investor: Grantová agentura ČR, Molekulární a buněčné principy embryonálního vývoje choroidního plexu

MUNI/A/1738/2024, interní kód MU

Název: Zdroje pro tkáňové inženýrství 15

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 15

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

LAVICKÝ, Josef; Marcos GONZÁLEZ LÓPEZ; Václav CHOCHOLA; Petra KOMPANÍKOVÁ; Vladislav RAKULTSEV; Jan RAŠKA; E Wentworth WINCHESTER; Haneen Riadh Ali TUAIMA; Lucie PEŠKOVÁ; Jan VERNER; Eva ŠVANDOVÁ; Marcela BUCHTOVÁ; J. COTNEY; Vítězslav BRYJA; Dáša BOHAČIAKOVÁ; Tomáš BÁRTA a Jan KŘIVÁNEK. Unraveling the Transcription Factor Code of Odontoblast Differentiation. Journal of Dental Research. Thousand Oaks: Sage Publication, 2026, roč. 105, č. 2, s. 256-266. ISSN 0022-0345. Dostupné z: https://doi.org/10.1177/00220345251348148.

@article{2511768,
   author = {Lavický, Josef and González López, Marcos and Chochola, Václav and Kompaníková, Petra and Rakultsev, Vladislav and Raška, Jan and Winchester, E Wentworth and Tuaima, Haneen Riadh Ali and Pešková, Lucie and Verner, Jan and Švandová, Eva and Buchtová, Marcela and Cotney, J. and Bryja, Vítězslav and Bohačiaková, Dáša and Bárta, Tomáš and Křivánek, Jan},
   article_location = {Thousand Oaks},
   article_number = {2},
   doi = {https://doi.org/10.1177/00220345251348148},
   keywords = {tooth development; odontogenesis; dentin; bioengineering; cell differentiation; stem cell(s)},
   language = {eng},
   issn = {0022-0345},
   journal = {Journal of Dental Research},
   title = {Unraveling the Transcription Factor Code of Odontoblast Differentiation},
   url = {https://journals.sagepub.com/doi/10.1177/00220345251348148},
   volume = {105},
   year = {2026}
}

TY  - JOUR
ID  - 2511768
AU  - Lavický, Josef - González López, Marcos - Chochola, Václav - Kompaníková, Petra - Rakultsev, Vladislav - Raška, Jan - Winchester, E Wentworth - Tuaima, Haneen Riadh Ali - Pešková, Lucie - Verner, Jan - Švandová, Eva - Buchtová, Marcela - Cotney, J. - Bryja, Vítězslav - Bohačiaková, Dáša - Bárta, Tomáš - Křivánek, Jan
PY  - 2026
TI  - Unraveling the Transcription Factor Code of Odontoblast Differentiation
JF  - Journal of Dental Research
VL  - 105
IS  - 2
SP  - 256-266
EP  - 256-266
PB  - Sage Publication
SN  - 00220345
KW  - tooth development
KW  - odontogenesis
KW  - dentin
KW  - bioengineering
KW  - cell differentiation
KW  - stem cell(s)
UR  - https://journals.sagepub.com/doi/10.1177/00220345251348148
N2  - The molecular mechanisms controlling the differentiation of stem cells into specialized cells and tissues are enormously complex. Deciphering the mechanisms behind this precisely regulated process is essential not only for understanding ontogenesis but also for interpretations of evolutionary dynamics. A deep understanding of differentiation processes in various cell types would open the way for safe and targeted tissue engineering. This was previously limited by technical constraints, but the recent development of multiomic approaches has overcome these barriers, providing unprecedented insight into the intricate workings of this molecular machinery. Based on single-cell RNA-seq analyses of continuously growing mouse teeth, 4 transcription factors (Etv4, Gsc, Sall1, and Nupr1) were selected. Their role during tooth development has not been previously evaluated. Here, we provide evidence about their function in the differentiation of odontoblasts-dental cells responsible for dentin production. Our results revealed that controlled overexpression of specific transcription factors directly in mouse-induced pluripotent stem cells is sufficient to guide their differentiation into cells with an odontoblast-like phenotype, both in vitro and in vivo. The differentiated cells exhibited upregulated expression of odontoblast-specific molecular markers (DMP1 and DSP) as well as production of collagenous and mineralized tissue. We demonstrate that a deep insight into fundamental developmental events can provide a powerful basis for innovative cell differentiation approaches. Taken together, this research might serve as a proof of concept for using large-omics data in the generation of specific, differentiated cell types by controlled expression of a particular transcription factor code. This may represent a turning point in both developmental biology and regenerative medicine fields.
ER  -

LAVICKÝ, Josef; Marcos GONZÁLEZ LÓPEZ; Václav CHOCHOLA; Petra KOMPANÍKOVÁ; Vladislav RAKULTSEV; Jan RAŠKA; E Wentworth WINCHESTER; Haneen Riadh Ali TUAIMA; Lucie PEŠKOVÁ; Jan VERNER; Eva ŠVANDOVÁ; Marcela BUCHTOVÁ; J. COTNEY; Vítězslav BRYJA; Dáša BOHAČIAKOVÁ; Tomáš BÁRTA a Jan KŘIVÁNEK. Unraveling the Transcription Factor Code of Odontoblast Differentiation. \textit{Journal of Dental Research}. Thousand Oaks: Sage Publication, 2026, roč.~105, č.~2, s.~256-266. ISSN~0022-0345. Dostupné z: https://doi.org/10.1177/00220345251348148.

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