Surface-Enhanced Raman Spectroscopy for Adenine Detection in
Five Selected Bacterial Strains Under Stress Conditions

J 2025

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

GHAZALOVÁ, Mona; Pavlína MODLITBOVÁ; Ota SAMEK; Katarína REBROŠOVÁ; Martin ŠILER et al.

Základní údaje

Originální název

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

Autoři

GHAZALOVÁ, Mona; Pavlína MODLITBOVÁ; Ota SAMEK; Katarína REBROŠOVÁ; Martin ŠILER; Jan JEŽEK a Zdeněk PILÁT

Vydání

Sensors, Basel, MDPI, 2025, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.500 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00141790

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

SERS; Ag-NPs; Au-NPs; phenylalanine; Escherichia coli; Staphylococcus aureus

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2026 08:53, Mgr. Tereza Miškechová

Anotace

V originále

This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor—demineralized water—inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.

Návaznosti

LM2023050, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech BioImaging: Národní výzkumná infrastruktura pro biologické a medicínské zobrazování

MUNI/A/1784/2024, interní kód MU

Název: Nové přístupy k diagnostice, prevenci a léčbě nozokomiálních patogenů

Investor: Masarykova univerzita, Nové přístupy k diagnostice, prevenci a léčbě nozokomiálních patogenů

Citovat

GHAZALOVÁ, Mona; Pavlína MODLITBOVÁ; Ota SAMEK; Katarína REBROŠOVÁ; Martin ŠILER; Jan JEŽEK a Zdeněk PILÁT. Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions. Sensors. Basel: MDPI, 2025, roč. 25, č. 15, s. 1-12. ISSN 1424-8220. Dostupné z: https://doi.org/10.3390/s25154629.

@article{2512118,
   author = {Ghazalová, Mona and Modlitbová, Pavlína and Samek, Ota and Rebrošová, Katarína and Šiler, Martin and Ježek, Jan and Pilát, Zdeněk},
   article_location = {Basel},
   article_number = {15},
   doi = {https://doi.org/10.3390/s25154629},
   keywords = {SERS; Ag-NPs; Au-NPs; phenylalanine; Escherichia coli; Staphylococcus aureus},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions},
   url = {https://www.mdpi.com/1424-8220/25/15/4629},
   volume = {25},
   year = {2025}
}

TY  - JOUR
ID  - 2512118
AU  - Ghazalová, Mona - Modlitbová, Pavlína - Samek, Ota - Rebrošová, Katarína - Šiler, Martin - Ježek, Jan - Pilát, Zdeněk
PY  - 2025
TI  - Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions
JF  - Sensors
VL  - 25
IS  - 15
SP  - 1-12
EP  - 1-12
PB  - MDPI
SN  - 14248220
KW  - SERS
KW  - Ag-NPs
KW  - Au-NPs
KW  - phenylalanine
KW  - Escherichia coli
KW  - Staphylococcus aureus
UR  - https://www.mdpi.com/1424-8220/25/15/4629
N2  - This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor—demineralized water—inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.
ER  -

GHAZALOVÁ, Mona; Pavlína MODLITBOVÁ; Ota SAMEK; Katarína REBROŠOVÁ; Martin ŠILER; Jan JEŽEK a Zdeněk PILÁT. Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions. \textit{Sensors}. Basel: MDPI, 2025, roč.~25, č.~15, s.~1-12. ISSN~1424-8220. Dostupné z: https://doi.org/10.3390/s25154629.

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