Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers
and Predictors of Residual Disease Activity and Relapse in
Treatment-Naïve Pediatric Inflammatory Bowel Disease

J 2025

Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers and Predictors of Residual Disease Activity and Relapse in Treatment-Naïve Pediatric Inflammatory Bowel Disease

DEISSOVÁ, Tereza; Dagmar AL TUKMACHI; Lenka RADOVÁ; Júlia BOHOŠOVÁ; Táňa MACHÁČKOVÁ et al.

Základní údaje

Originální název

Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers and Predictors of Residual Disease Activity and Relapse in Treatment-Naïve Pediatric Inflammatory Bowel Disease

Autoři

Vydání

Inflammatory Bowel Diseases, Oxford University Press, 2025, 1078-0998

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30219 Gastroenterology and hepatology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00141904

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

inflammatory bowel disease; microRNA markers; pediatric

Štítky

14110230, 14110317, 14110513, 14313050, CF BIOIT, CF GEN

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 1. 2026 14:55, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background Identifying novel diagnostic and prognostic biomarkers for pediatric inflammatory bowel diseases (PIBD), including Crohn's disease (pCD) and ulcerative colitis (pUC), is essential for enhancing treatment outcomes. MicroRNAs (miRNAs) have been recognized for their broader relevance in PIBD pathogenesis. This study investigates their diagnostic potential and clinical utility in PIBD.Methods This prospective, monocentric study, with retrospective validation, involved 119 PIBD patients (58 pCD, 61 pUC) and 39 non-IBD controls. Small RNA next-generation sequencing was performed on fresh-frozen gut biopsies, targeting histopathologically confirmed inflamed areas. Twenty-five dysregulated miRNA candidates were validated via RT-qPCR in formalin-fixed, paraffin-embedded gut biopsies. Logistic regression was used to establish diagnostic and prognostic miRNA expression signatures.Results A diagnostic signature of 5 miRNAs (miR-223-3p, miR-34a-5p, miR-194-5p, miR-215-5p, miR-338-3p) distinguished pCD from non-IBD with 96.49% accuracy. Two miRNAs (miR-223-3p, miR-194-5p) differentiated pUC from non-IBD with 100% accuracy, and miR-215-5p distinguished pCD from pUC specimens with 83.54% accuracy. For treatment-na & iuml;ve pCD patients, 7 miRNAs predicted residual disease activity at 3 months with 100% accuracy. Additionally, a distinct signature predicted the risk of relapse within 12 months with an accuracy of 84.21%.Conclusions In this study, we have established tissue miRNA expression signatures with significant diagnostic and prognostic potential for use in PIBD. These findings aid in stratifying disease severity and risk, paving the way for more precise and personalized management of pediatric IBD.

Návaznosti

LM2023067, projekt VaV

Název: Národní centrum lékařské genomiky

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, NCLG: Národní centrum lékařské genomiky

NU21-07-00285, projekt VaV

Název: Studium mikrobiální dysbiózy a deregulace mikroRNA pro účely individualizace terapeutického přístupu k dětským pacientům s nespecifickými střevními záněty

Investor: Ministerstvo zdravotnictví ČR, Studium mikrobiální dysbiózy a deregulace mikroRNA pro účely individualizace terapeutického přístupu k dětským pacientům s nespecifickými střevními záněty, Podprogram 1 - standardní

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

DEISSOVÁ, Tereza; Dagmar AL TUKMACHI; Lenka RADOVÁ; Júlia BOHOŠOVÁ; Táňa MACHÁČKOVÁ; Leoš KŘEN; Matěj HRUNKA; Tereza PINKASOVÁ; Martina AMBROZOVÁ; Jiří ŠÁNA; Ondřej SLABÝ a Petr JABANDŽIEV. Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers and Predictors of Residual Disease Activity and Relapse in Treatment-Naïve Pediatric Inflammatory Bowel Disease. Inflammatory Bowel Diseases. Oxford University Press, 2025, roč. 31, č. 11, s. 3033-3043. ISSN 1078-0998. Dostupné z: https://doi.org/10.1093/ibd/izaf120.

@article{2514920,
   author = {Deissová, Tereza and Al Tukmachi, Dagmar and Radová, Lenka and Bohošová, Júlia and Macháčková, Táňa and Křen, Leoš and Hrunka, Matěj and Pinkasová, Tereza and Ambrozová, Martina and Šána, Jiří and Slabý, Ondřej and Jabandžiev, Petr},
   article_number = {11},
   doi = {https://doi.org/10.1093/ibd/izaf120},
   keywords = {inflammatory bowel disease; microRNA markers; pediatric},
   language = {eng},
   issn = {1078-0998},
   journal = {Inflammatory Bowel Diseases},
   title = {Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers and Predictors of Residual Disease Activity and Relapse in Treatment-Naïve Pediatric Inflammatory Bowel Disease},
   url = {https://academic.oup.com/ibdjournal/advance-article/doi/10.1093/ibd/izaf120/8242092?login=true},
   volume = {31},
   year = {2025}
}

TY  - JOUR
ID  - 2514920
AU  - Deissová, Tereza - Al Tukmachi, Dagmar - Radová, Lenka - Bohošová, Júlia - Macháčková, Táňa - Křen, Leoš - Hrunka, Matěj - Pinkasová, Tereza - Ambrozová, Martina - Šána, Jiří - Slabý, Ondřej - Jabandžiev, Petr
PY  - 2025
TI  - Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers and Predictors of Residual Disease Activity and Relapse in Treatment-Naïve Pediatric Inflammatory Bowel Disease
JF  - Inflammatory Bowel Diseases
VL  - 31
IS  - 11
SP  - 3033-3043
EP  - 3033-3043
PB  - Oxford University Press
SN  - 10780998
KW  - inflammatory bowel disease
KW  - microRNA markers
KW  - pediatric
UR  - https://academic.oup.com/ibdjournal/advance-article/doi/10.1093/ibd/izaf120/8242092?login=true
N2  - Background Identifying novel diagnostic and prognostic biomarkers for pediatric inflammatory bowel diseases (PIBD), including Crohn's disease (pCD) and ulcerative colitis (pUC), is essential for enhancing treatment outcomes. MicroRNAs (miRNAs) have been recognized for their broader relevance in PIBD pathogenesis. This study investigates their diagnostic potential and clinical utility in PIBD.Methods This prospective, monocentric study, with retrospective validation, involved 119 PIBD patients (58 pCD, 61 pUC) and 39 non-IBD controls. Small RNA next-generation sequencing was performed on fresh-frozen gut biopsies, targeting histopathologically confirmed inflamed areas. Twenty-five dysregulated miRNA candidates were validated via RT-qPCR in formalin-fixed, paraffin-embedded gut biopsies. Logistic regression was used to establish diagnostic and prognostic miRNA expression signatures.Results A diagnostic signature of 5 miRNAs (miR-223-3p, miR-34a-5p, miR-194-5p, miR-215-5p, miR-338-3p) distinguished pCD from non-IBD with 96.49% accuracy. Two miRNAs (miR-223-3p, miR-194-5p) differentiated pUC from non-IBD with 100% accuracy, and miR-215-5p distinguished pCD from pUC specimens with 83.54% accuracy. For treatment-na & iuml;ve pCD patients, 7 miRNAs predicted residual disease activity at 3 months with 100% accuracy. Additionally, a distinct signature predicted the risk of relapse within 12 months with an accuracy of 84.21%.Conclusions In this study, we have established tissue miRNA expression signatures with significant diagnostic and prognostic potential for use in PIBD. These findings aid in stratifying disease severity and risk, paving the way for more precise and personalized management of pediatric IBD.
ER  -

DEISSOVÁ, Tereza; Dagmar AL TUKMACHI; Lenka RADOVÁ; Júlia BOHOŠOVÁ; Táňa MACHÁČKOVÁ; Leoš KŘEN; Matěj HRUNKA; Tereza PINKASOVÁ; Martina AMBROZOVÁ; Jiří ŠÁNA; Ondřej SLABÝ a Petr JABANDŽIEV. Tissue MicroRNA Expression Signatures as Diagnostic Biomarkers and Predictors of Residual Disease Activity and Relapse in Treatment-Naïve Pediatric Inflammatory Bowel Disease. \textit{Inflammatory Bowel Diseases}. Oxford University Press, 2025, roč.~31, č.~11, s.~3033-3043. ISSN~1078-0998. Dostupné z: https://doi.org/10.1093/ibd/izaf120.

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