Bioconjugates of Photon-Upconversion Nanoparticles as
Background-Free Labels for Immunosensing and Imaging

l 2025

Bioconjugates of Photon-Upconversion Nanoparticles as Background-Free Labels for Immunosensing and Imaging

FARKA, Zdeněk; Antonín HLAVÁČEK; Dorota SKLENÁROVÁ a Hans-Heiner GORRIS

Základní údaje

Originální název

Bioconjugates of Photon-Upconversion Nanoparticles as Background-Free Labels for Immunosensing and Imaging

Autoři

FARKA, Zdeněk; Antonín HLAVÁČEK; Dorota SKLENÁROVÁ a Hans-Heiner GORRIS

Vydání

XXVIIIth Biochemistry Congress, s. 72-72, 2025

Nakladatel

Venice Praha

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku (neindexovaná)

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-907779-5-8

Příznaky

Mezinárodní význam

Změněno: 11. 2. 2026 15:11, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that exhibit anti-Stokes luminescence, enabling their detection without optical background interferences. Therefore, conjugates of UCNPs with biorecognition molecules are well-suited as labels in immunochemical sensing and imaging. Our work explores different ways of modifying the UCNP surface and employs the conjugates to develop immunoassays for a wide range of analytes, from small molecules to bacteria. As part of our research on cancer biomarker detection, we developed a single-molecule immunoassay for prostate-specific antigen based on counting individual immunocomplexes under a wide-field epiluminescence microscope. This work was followed by transferring the assay from the surface of a microtiter plate to magnetic microparticles for sample preconcentration, and we are currently exploring advanced assay formats to increase the assay specificity and sensitivity. Furthermore, we employ the UCNP bioconjugates for high-contrast visualization of biomarkers on cancer cells. Due to the insignificant optical background and low non-specific interactions, the upconversion readout provides a superior signal-to-background ratio compared to the fluorescence-based detection. We are also exploring other readout types. For example, laser-induced breakdown spectroscopy detects individual elements in UCNPs, enhancing the multiplexing capabilities.

Návaznosti

GA21-03156S, projekt VaV

Název: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Grantová agentura ČR, Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Citovat

FARKA, Zdeněk; Antonín HLAVÁČEK; Dorota SKLENÁROVÁ a Hans-Heiner GORRIS. Bioconjugates of Photon-Upconversion Nanoparticles as Background-Free Labels for Immunosensing and Imaging. In Libor Grubhoffer et al. XXVIIIth Biochemistry Congress. Venice Praha, 2025, s. 72. ISBN 978-80-907779-5-8.

@inproceedings{2518037,
   author = {Farka, Zdeněk and Hlaváček, Antonín and Sklenárová, Dorota and Gorris, HansandHeiner},
   booktitle = {XXVIIIth Biochemistry Congress},
   editor = {Libor Grubhoffer et al.},
   howpublished = {tištěná verze "print"},
   language = {eng},
   isbn = {978-80-907779-5-8},
   pages = {72-72},
   publisher = {Venice Praha},
   title = {Bioconjugates of Photon-Upconversion Nanoparticles as Background-Free Labels for Immunosensing and Imaging},
   year = {2025}
}

TY  - CONF
ID  - 2518037
AU  - Farka, Zdeněk - Hlaváček, Antonín - Sklenárová, Dorota - Gorris, Hans-Heiner
PY  - 2025
TI  - Bioconjugates of Photon-Upconversion Nanoparticles as Background-Free Labels for Immunosensing and Imaging
PB  - Venice Praha
SN  - 9788090777958
N2  - Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that exhibit anti-Stokes luminescence, enabling their detection without optical background interferences. Therefore, conjugates of UCNPs with biorecognition molecules are well-suited as labels in immunochemical sensing and imaging. Our work explores different ways of modifying the UCNP surface and employs the conjugates to develop immunoassays for a wide range of analytes, from small molecules to bacteria. As part of our research on cancer biomarker detection, we developed a single-molecule immunoassay for prostate-specific antigen based on counting individual immunocomplexes under a wide-field epiluminescence microscope. This work was followed by transferring the assay from the surface of a microtiter plate to magnetic microparticles for sample preconcentration, and we are currently exploring advanced assay formats to increase the assay specificity and sensitivity. Furthermore, we employ the UCNP bioconjugates for high-contrast visualization of biomarkers on cancer cells. Due to the insignificant optical background and low non-specific interactions, the upconversion readout provides a superior signal-to-background ratio compared to the fluorescence-based detection. We are also exploring other readout types. For example, laser-induced breakdown spectroscopy detects individual elements in UCNPs, enhancing the multiplexing capabilities.
ER  -

FARKA, Zdeněk; Antonín HLAVÁČEK; Dorota SKLENÁROVÁ a Hans-Heiner GORRIS. Bioconjugates of Photon-Upconversion Nanoparticles as Background-Free Labels for Immunosensing and Imaging. In Libor Grubhoffer et al. \textit{XXVIIIth Biochemistry Congress}. Venice Praha, 2025, s.~72. ISBN~978-80-907779-5-8.

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