Spatiotemporal mapping of bone remodeling: Effects of physical
activity and mechanical unloading

a 2025

Spatiotemporal mapping of bone remodeling: Effects of physical activity and mechanical unloading

CIGOŠOVÁ, Klára; Alexandra BALAJOVÁ; Michaela KAVKOVÁ; Nela GAJDUŠKOVÁ; Sabina CERULOVÁ et al.

Základní údaje

Originální název

Spatiotemporal mapping of bone remodeling: Effects of physical activity and mechanical unloading

Autoři

Vydání

Visegrád Group Society for Developmental Biology Meeting 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10605 Developmental biology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Změněno: 15. 9. 2025 14:34, Mgr. Tereza Slaměníková

Anotace

V originále

Bone is a dynamic tissue that undergoes continuous process of resorption and deposition—bone remodeling. It is regulated not only by metabolic demands such as calcium and phosphorus physiological balance, but importantly also by current mechanical needs. During development and adulthood, mechanical loading plays a key role in shaping bone structure, function, and adaptation. Conversely, mechanical unloading—such as that occurring during injury, aging, or prolonged bed rest—can disrupt this balance, compromising bone quality and skeletal health. While the effects of mechanical forces and structured physical training on bone turnover have been previously studied, spatiotemporal dynamics remain poorly understood. To address this, we investigated how reduced mechanical load and targeted physical activity influence bone properties, morphology, and remodeling dynamics in adult female Wistar rats. We implemented a complex experimental design incorporating endurance (weight-bearing) and resistance (treadmill) training, followed by hindlimb unloading and partial weight-bearing to mimic mechanical disuse. In vivo assessments included training performance and body composition via echo MRI. To capture remodeling in space and time, we applied the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach on femur, humerus, and calvaria. This was complemented by high-resolution micro-computed tomography (μCT) for morphometric and mechanical evaluation, and laser-induced breakdown spectroscopy (LIBS) to assess elemental composition shifts. Our results show that preconditioning through physical training significantly mitigates the effects of unloading, preserving bone density, morphology, and load-bearing capacity. Notably, trained animals could support up to three times their body weight, with this capacity declining post-unloading. Moreover, unloading alone led to a marked increase in resorption. This study highlights the role of activity-dependent skeletal adaptation and, for the first time, maps the spatiotemporal dynamics of bone remodeling in response to mechanical alternations. These findings also underscore the potential of this approach for investigating bone development and plasticity.

Návaznosti

GA25-18087S, projekt VaV

Název: Buněčná podstata hojení zubu

Investor: Grantová agentura ČR, Buněčná podstata hojení zubu

Citovat

CIGOŠOVÁ, Klára; Alexandra BALAJOVÁ; Michaela KAVKOVÁ; Nela GAJDUŠKOVÁ; Sabina CERULOVÁ; Marcos GONZÁLEZ LÓPEZ; Josef LAVICKÝ; Pavel POŘÍZKA; Karolína VAŇKOVÁ; Thomas BRIOCHE; Hana KOPŘIVOVÁ; Julian THEUIL; Margot ISSERTINE; Angèle CHOPARD a Jan KŘIVÁNEK. Spatiotemporal mapping of bone remodeling: Effects of physical activity and mechanical unloading. In Visegrád Group Society for Developmental Biology Meeting 2025. 2025.

@proceedings{2518604,
   author = {Cigošová, Klára and Balajová, Alexandra and Kavková, Michaela and Gajdušková, Nela and Cerulová, Sabina and González López, Marcos and Lavický, Josef and Pořízka, Pavel and Vaňková, Karolína and Brioche, Thomas and Kopřivová, Hana and Theuil, Julian and Issertine, Margot and Chopard, Angèle and Křivánek, Jan},
   booktitle = {Visegrád Group Society for Developmental Biology Meeting 2025},
   language = {eng},
   title = {Spatiotemporal mapping of bone remodeling: Effects of physical activity and mechanical unloading},
   url = {https://v4sdb2025.img.cas.cz/},
   year = {2025}
}

TY  - CONF
ID  - 2518604
AU  - Cigošová, Klára - Balajová, Alexandra - Kavková, Michaela - Gajdušková, Nela - Cerulová, Sabina - González López, Marcos - Lavický, Josef - Pořízka, Pavel - Vaňková, Karolína - Brioche, Thomas - Kopřivová, Hana - Theuil, Julian - Issertine, Margot - Chopard, Angèle - Křivánek, Jan
PY  - 2025
TI  - Spatiotemporal mapping of bone remodeling: Effects of physical activity and mechanical unloading
UR  - https://v4sdb2025.img.cas.cz/
N2  - Bone is a dynamic tissue that undergoes continuous process of resorption and deposition—bone remodeling. It is regulated not only by metabolic demands such as calcium and phosphorus physiological balance, but importantly also by current mechanical needs. During development and adulthood, mechanical loading plays a key role in shaping bone structure, function, and adaptation. Conversely, mechanical unloading—such as that occurring during injury, aging, or prolonged bed rest—can disrupt this balance, compromising bone quality and skeletal health. While the effects of mechanical forces and structured physical training on bone turnover have been previously studied, spatiotemporal dynamics remain poorly understood. To address this, we investigated how reduced mechanical load and targeted physical activity influence bone properties, morphology, and remodeling dynamics in adult female Wistar rats. We implemented a complex experimental design incorporating endurance (weight-bearing) and resistance (treadmill) training, followed by hindlimb unloading and partial weight-bearing to mimic mechanical disuse. In vivo assessments included training performance and body composition via echo MRI. To capture remodeling in space and time, we applied the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach on femur, humerus, and calvaria. This was complemented by high-resolution micro-computed tomography (μCT) for morphometric and mechanical evaluation, and laser-induced breakdown spectroscopy (LIBS) to assess elemental composition shifts. Our results show that preconditioning through physical training significantly mitigates the effects of unloading, preserving bone density, morphology, and load-bearing capacity. Notably, trained animals could support up to three times their body weight, with this capacity declining post-unloading. Moreover, unloading alone led to a marked increase in resorption. This study highlights the role of activity-dependent skeletal adaptation and, for the first time, maps the spatiotemporal dynamics of bone remodeling in response to mechanical alternations. These findings also underscore the potential of this approach for investigating bone development and plasticity.
ER  -

CIGOŠOVÁ, Klára; Alexandra BALAJOVÁ; Michaela KAVKOVÁ; Nela GAJDUŠKOVÁ; Sabina CERULOVÁ; Marcos GONZÁLEZ LÓPEZ; Josef LAVICKÝ; Pavel POŘÍZKA; Karolína VAŇKOVÁ; Thomas BRIOCHE; Hana KOPŘIVOVÁ; Julian THEUIL; Margot ISSERTINE; Angèle CHOPARD a Jan KŘIVÁNEK. Spatiotemporal mapping of bone remodeling: Effects of physical activity and mechanical unloading. In \textit{Visegrád Group Society for Developmental Biology Meeting 2025}. 2025.

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