Reduced inducibility of SOCS3 by interferon gamma associates
with higher resistance of human breast cancer lines as compared
to normal mammary epithelial cells

J 2009

Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells

SOUČKOVÁ, Kamila; A. KOVARIK; Ladislav DUSEK; L. HUMPOLIKOVA-ADAMKOVA; L. LAUEROVA et al.

Základní údaje

Originální název

Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells

Autoři

SOUČKOVÁ, Kamila ; A. KOVARIK; Ladislav DUSEK; L. HUMPOLIKOVA-ADAMKOVA; L. LAUEROVA; E. KREJCI; E. MATOUSKOVA; E. BURSIKOVA; Miloslava FOJTOVÁ a J. KOVARIK

Vydání

NEOPLASMA, BRATISLAVA, AEPRESS SRO, 2009, 0028-2685

Další údaje

Typ výsledku

Článek v odborném periodiku

Impakt faktor

Impact factor: 1.192

Označené pro přenos do RIV

DOI

https://doi.org/10.4149/neo_2009_05_379

UT WoS

000269333200002

Klíčová slova anglicky

suppressor of STAT signaling; STAT1; breast cancer; interferon; cell resistance

Změněno: 17. 9. 2025 16:22, RNDr. Kamila Součková, Ph.D.

Anotace

V originále

The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the antiproliferative effect of interferon gamma in relation to SOCS3 expression in a panel of breast cancer cell lines and normal mammary epithelial cells. Compared to normal cells most breast cancer lines (7/8) were highly resistant to IFN-gamma. Using Northern blot and real time RT-PCR we investigated transcription of SOCS3 genes. All normal epithelial cells (4/4) showed SOCS3 induction (2-14 fold) while most breast cancer lines did not or weakly activated SOCS3 after the interferon gamma treatment. Among the cancer lines, the MDA-MB-468 cells showed increased sensitivity to IFN-gamma and relatively high level of SOCS3 induction (2-3 fold). Together, there was a good correlation (P < 0.001) between SOCS3 inducibility and growth inhibition by IFN-gamma. The correlations between sensitivity and STAT1 phosphorylation levels (pY-701) were less pronounced since there were cases of STAT1-pY induction (2/12) without apparent growth inhibitory effect. The data indicate defective STAT1 phosphorylation and SOCS3 induction in breast cancer cells. These defects likely contribute to reduced sensitivity of malignant cells to antiproliferative effect of IFN-gamma.

Citovat

SOUČKOVÁ, Kamila; A. KOVARIK; Ladislav DUSEK; L. HUMPOLIKOVA-ADAMKOVA; L. LAUEROVA; E. KREJCI; E. MATOUSKOVA; E. BURSIKOVA; Miloslava FOJTOVÁ a J. KOVARIK. Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells. NEOPLASMA. BRATISLAVA: AEPRESS SRO, 2009, roč. 56, č. 5, s. 379-386. ISSN 0028-2685. Dostupné z: https://doi.org/10.4149/neo_2009_05_379.

@article{2519299,
   author = {Součková, Kamila and Kovarik, A. and Dusek, Ladislav and HumpolikovaandAdamkova, L. and Lauerova, L. and Krejci, E. and Matouskova, E. and Bursikova, E. and Fojtová, Miloslava and Kovarik, J.},
   article_location = {BRATISLAVA},
   article_number = {5},
   doi = {https://doi.org/10.4149/neo_2009_05_379},
   keywords = {suppressor of STAT signaling; STAT1; breast cancer; interferon; cell resistance},
   issn = {0028-2685},
   journal = {NEOPLASMA},
   title = {Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells},
   volume = {56},
   year = {2009}
}

TY  - JOUR
ID  - 2519299
AU  - Součková, Kamila - Kovarik, A. - Dusek, Ladislav - Humpolikova-Adamkova, L. - Lauerova, L. - Krejci, E. - Matouskova, E. - Bursikova, E. - Fojtová, Miloslava - Kovarik, J.
PY  - 2009
TI  - Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells
JF  - NEOPLASMA
VL  - 56
IS  - 5
SP  - 379-386
EP  - 379-386
PB  - AEPRESS SRO
SN  - 00282685
KW  - suppressor of STAT signaling
KW  - STAT1
KW  - breast cancer
KW  - interferon
KW  - cell resistance
N2  - The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the antiproliferative effect of interferon gamma in relation to SOCS3 expression in a panel of breast cancer cell lines and normal mammary epithelial cells. Compared to normal cells most breast cancer lines (7/8) were highly resistant to IFN-gamma. Using Northern blot and real time RT-PCR we investigated transcription of SOCS3 genes. All normal epithelial cells (4/4) showed SOCS3 induction (2-14 fold) while most breast cancer lines did not or weakly activated SOCS3 after the interferon gamma treatment. Among the cancer lines, the MDA-MB-468 cells showed increased sensitivity to IFN-gamma and relatively high level of SOCS3 induction (2-3 fold). Together, there was a good correlation (P < 0.001) between SOCS3 inducibility and growth inhibition by IFN-gamma. The correlations between sensitivity and STAT1 phosphorylation levels (pY-701) were less pronounced since there were cases of STAT1-pY induction (2/12) without apparent growth inhibitory effect. The data indicate defective STAT1 phosphorylation and SOCS3 induction in breast cancer cells. These defects likely contribute to reduced sensitivity of malignant cells to antiproliferative effect of IFN-gamma.
ER  -

SOUČKOVÁ, Kamila; A. KOVARIK; Ladislav DUSEK; L. HUMPOLIKOVA-ADAMKOVA; L. LAUEROVA; E. KREJCI; E. MATOUSKOVA; E. BURSIKOVA; Miloslava FOJTOVÁ a J. KOVARIK. Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells. \textit{NEOPLASMA}. BRATISLAVA: AEPRESS SRO, 2009, roč.~56, č.~5, s.~379-386. ISSN~0028-2685. Dostupné z: https://doi.org/10.4149/neo\_{}2009\_{}05\_{}379.

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