Biomethane Production from Stillage: The Role of Temperature,
Initial Substrate Load, and Inoculum Variability

J 2025

Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability

VÍTĚZ, Tomáš; Nikola HANIŠÁKOVÁ; Pavel SUCHÝ; Petra TEJCHMANOVÁ; Jan KUDĚLKA et al.

Základní údaje

Originální název

Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability

Autoři

VÍTĚZ, Tomáš ; Nikola HANIŠÁKOVÁ; Pavel SUCHÝ; Petra TEJCHMANOVÁ; Jan KUDĚLKA; Tomáš KOUTNÝ; Jan LOCHMAN; David NOVÁK a Monika VÍTĚZOVÁ

Vydání

ACS Omega, American Chemical Society, 2025, 2470-1343

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20700 2.7 Environmental engineering

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00142069

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Fermentation; Hydrocarbons; Plants; Wastewater; Water treatment

Štítky

14313050, 14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 11. 2025 10:58, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Molasses stillage, with its high organic content and nutrient-rich composition, represents a promising feedstock for biogas production. This study systematically evaluated its biochemical methane potential (BMP) using two inocula (wastewater treatment plant vs agricultural biogas plant) across three temperatures (40, 50, 60 °C) and initial substrate load (ISL: 2, 5, 10 g·Lincolum–1). The wastewater inoculum achieved superior methane yields (0.262–0.477 N m3·kgvs–1), peaking at 50 °C with a 22% increase over agricultural systems (0.192–0.378 N m3·kgvs–1). 16S rRNA sequencing revealed the wastewater treatment plant inoculum’s superior functional diversity, dominated by syntrophic Chloroflexota and Acidobacteriota alongside methanogenic Methanobacterium (52–61% relative abundance) and acetoclastic Methanothrix (18–23%). In contrast, agricultural biogas plant inocula showed specialized thermophilic communities dominated by Bacillota (68–72%) and hydrogenotrophic Methanoculleus (29–34%). Both systems exhibited inhibition at 60 °C/ISL10 (yields reduced by 34–42%), correlating with declining diversity and Methanofastidiosum proliferation. These findings provide two key operational insights: wastewater inocula offer greater process stability due to microbial diversity, and 50 °C represents the thermal optimum for stillage codigestion, balancing yield and community resilience.

Citovat

VÍTĚZ, Tomáš; Nikola HANIŠÁKOVÁ; Pavel SUCHÝ; Petra TEJCHMANOVÁ; Jan KUDĚLKA; Tomáš KOUTNÝ; Jan LOCHMAN; David NOVÁK a Monika VÍTĚZOVÁ. Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability. ACS Omega. American Chemical Society, 2025, roč. 10, č. 38, s. 43687-43697. ISSN 2470-1343. Dostupné z: https://doi.org/10.1021/acsomega.5c03798.

@article{2519822,
   author = {Vítěz, Tomáš and Hanišáková, Nikola and Suchý, Pavel and Tejchmanová, Petra and Kudělka, Jan and Koutný, Tomáš and Lochman, Jan and Novák, David and Vítězová, Monika},
   article_number = {38},
   doi = {https://doi.org/10.1021/acsomega.5c03798},
   keywords = {Fermentation; Hydrocarbons; Plants; Wastewater; Water treatment},
   language = {eng},
   issn = {2470-1343},
   journal = {ACS Omega},
   title = {Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability},
   url = {https://pubs.acs.org/doi/10.1021/acsomega.5c03798},
   volume = {10},
   year = {2025}
}

TY  - JOUR
ID  - 2519822
AU  - Vítěz, Tomáš - Hanišáková, Nikola - Suchý, Pavel - Tejchmanová, Petra - Kudělka, Jan - Koutný, Tomáš - Lochman, Jan - Novák, David - Vítězová, Monika
PY  - 2025
TI  - Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability
JF  - ACS Omega
VL  - 10
IS  - 38
SP  - 43687-43697
EP  - 43687-43697
PB  - American Chemical Society
SN  - 24701343
KW  - Fermentation
KW  - Hydrocarbons
KW  - Plants
KW  - Wastewater
KW  - Water treatment
UR  - https://pubs.acs.org/doi/10.1021/acsomega.5c03798
N2  - Molasses stillage, with its high organic content and nutrient-rich composition, represents a promising feedstock for biogas production. This study systematically evaluated its biochemical methane potential (BMP) using two inocula (wastewater treatment plant vs agricultural biogas plant) across three temperatures (40, 50, 60 °C) and initial substrate load (ISL: 2, 5, 10 g·Lincolum–1). The wastewater inoculum achieved superior methane yields (0.262–0.477 N m3·kgvs–1), peaking at 50 °C with a 22% increase over agricultural systems (0.192–0.378 N m3·kgvs–1). 16S rRNA sequencing revealed the wastewater treatment plant inoculum’s superior functional diversity, dominated by syntrophic Chloroflexota and Acidobacteriota alongside methanogenic Methanobacterium (52–61% relative abundance) and acetoclastic Methanothrix (18–23%). In contrast, agricultural biogas plant inocula showed specialized thermophilic communities dominated by Bacillota (68–72%) and hydrogenotrophic Methanoculleus (29–34%). Both systems exhibited inhibition at 60 °C/ISL10 (yields reduced by 34–42%), correlating with declining diversity and Methanofastidiosum proliferation. These findings provide two key operational insights: wastewater inocula offer greater process stability due to microbial diversity, and 50 °C represents the thermal optimum for stillage codigestion, balancing yield and community resilience.
ER  -

VÍTĚZ, Tomáš; Nikola HANIŠÁKOVÁ; Pavel SUCHÝ; Petra TEJCHMANOVÁ; Jan KUDĚLKA; Tomáš KOUTNÝ; Jan LOCHMAN; David NOVÁK a Monika VÍTĚZOVÁ. Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability. \textit{ACS Omega}. American Chemical Society, 2025, roč.~10, č.~38, s.~43687-43697. ISSN~2470-1343. Dostupné z: https://doi.org/10.1021/acsomega.5c03798.

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