J 2016

Genome-Editing Technologies: Principles and Applications

GAJ, Thomas; Shannon J. SIRK; Sai-lan SHUI a Jia LIU

Základní údaje

Originální název

Genome-Editing Technologies: Principles and Applications

Název česky

Technologie editování genomu: principy a aplikace

Název anglicky

Genome-Editing Technologies: Principles and Applications

Autoři

GAJ, Thomas; Shannon J. SIRK; Sai-lan SHUI a Jia LIU

Vydání

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, COLD SPRING HARBOR, COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2016, 1943-0264

Další údaje

Typ výsledku

Článek v odborném periodiku

Impakt faktor

Impact factor: 8.769

DOI

UT WoS

000390367600003
Změněno: 29. 9. 2025 13:50, Mgr. Jiřina Medalová, Ph.D.

Anotace

ORIG EN

V originále

Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy. Here we review three foundational technologies-clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). We discuss the engineering advances that facilitated their development and highlight several achievements in genome engineering that were made possible by these tools. We also consider artificial transcription factors, illustrating how this technology can complement targeted nucleases for synthetic biology and gene therapy.

Anglicky

Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy. Here we review three foundational technologies-clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). We discuss the engineering advances that facilitated their development and highlight several achievements in genome engineering that were made possible by these tools. We also consider artificial transcription factors, illustrating how this technology can complement targeted nucleases for synthetic biology and gene therapy.