Integrative taxonomy reveals Parakaliella harimensis (Pilsbry,
1901) as a distinct East Asian Euconulus species with deep
genetic structure

J 2026

Integrative taxonomy reveals Parakaliella harimensis (Pilsbry, 1901) as a distinct East Asian Euconulus species with deep genetic structure

KAFIMOLA, Sara; Veronika HORSÁKOVÁ; Markéta NOVÁKOVÁ; Eva MIKULÁŠKOVÁ; Kazuki KIMURA et al.

Základní údaje

Originální název

Integrative taxonomy reveals Parakaliella harimensis (Pilsbry, 1901) as a distinct East Asian Euconulus species with deep genetic structure

Autoři

KAFIMOLA, Sara; Veronika HORSÁKOVÁ; Markéta NOVÁKOVÁ; Eva MIKULÁŠKOVÁ; Kazuki KIMURA; Stefan MENG; Jan DIVÍŠEK; Jeffrey Clark NEKOLA a Michal HORSÁK

Vydání

Molecular Phylogenetics and Evolution, Elsevier, 2026, 1055-7903

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10602 Biology , Evolutionary biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.600 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Distribution; Integrative taxonomy; Land snails; New markers; Phylogeny; Stability

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 10. 2025 13:15, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The remarkable phenotypic plasticity of land snail shells often results in convergent evolution, leading to frequent taxonomic misidentifications and non-monophyletic classifications. The taxonomy of the Holarctic micro land snails related to Euconulus fulvus has been particularly challenging to resolve. This study integrates mitochondrial and nuclear DNA phylogenetics, geometric morphometrics, and climate suitability modeling to clarify the phylogenetic and taxonomic status of an East Asian lineage within this group. We confirm that this lineage represents a distinct and previously misclassified species, Euconulus harimensis, which was formerly placed within the genus Parakaliella. While standard mtDNA and nDNA markers showed this evolutionary lineage as polyphyletic, two newly developed nuclear markers (ZN507 and TEP1) resolved the lineage, except for one individual, within a single robust clade of E. harimensis. Geometric morphometric analyses revealed significant differences in shell shape, which, along with unique shell microsculpture, further support its taxonomic separation. Although E. harimensis possesses the smallest distribution range of all related species, it exhibits the highest intraspecific genetic diversity. The models of suitable climatic conditions for both the present and the Last Glacial Maximum showed no major shifts, suggesting high climatic stability of the species range during the Pleistocene. This, along with other palaeobiogeographic factors, may have contributed to the unexpectedly high intraspecific genetic diversity of this species despite its limited range. Our findings provide new insights into the evolutionary processes that shape land snail inter- and intraspecific diversity, with implications for their taxonomy, which has often relied on shell morphology only. Additionally, phylogenetic analysis based on the new markers sheds novel light on the taxonomy and evolution of E. fulvus and E. alderi, highlighting the need for further taxonomic research.

Návaznosti

GA25-15569S, projekt VaV

Název: Biodiverzita v souvislosti se stabilitou podmínek: test založený na společenstvech a genetice plžů boreálních oceanických oblastí

Investor: Grantová agentura ČR, Biodiverzita v souvislosti se stabilitou podmínek: test založený na společenstvech a genetice plžů boreálních oceanických oblastí

Citovat

KAFIMOLA, Sara; Veronika HORSÁKOVÁ; Markéta NOVÁKOVÁ; Eva MIKULÁŠKOVÁ; Kazuki KIMURA; Stefan MENG; Jan DIVÍŠEK; Jeffrey Clark NEKOLA a Michal HORSÁK. Integrative taxonomy reveals Parakaliella harimensis (Pilsbry, 1901) as a distinct East Asian Euconulus species with deep genetic structure. Molecular Phylogenetics and Evolution. Elsevier, 2026, roč. 214, January, s. "108460", 11 s. ISSN 1055-7903. Dostupné z: https://doi.org/10.1016/j.ympev.2025.108460.

@article{2523147,
   author = {Kafimola, Sara and Horsáková, Veronika and Nováková, Markéta and Mikulášková, Eva and Kimura, Kazuki and Meng, Stefan and Divíšek, Jan and Nekola, Jeffrey Clark and Horsák, Michal},
   article_number = {January},
   doi = {https://doi.org/10.1016/j.ympev.2025.108460},
   keywords = {Distribution; Integrative taxonomy; Land snails; New markers; Phylogeny; Stability},
   language = {eng},
   issn = {1055-7903},
   journal = {Molecular Phylogenetics and Evolution},
   title = {Integrative taxonomy reveals Parakaliella harimensis (Pilsbry, 1901) as a distinct East Asian Euconulus species with deep genetic structure},
   url = {https://doi.org/10.1016/j.ympev.2025.108460},
   volume = {214},
   year = {2026}
}

TY  - JOUR
ID  - 2523147
AU  - Kafimola, Sara - Horsáková, Veronika - Nováková, Markéta - Mikulášková, Eva - Kimura, Kazuki - Meng, Stefan - Divíšek, Jan - Nekola, Jeffrey Clark - Horsák, Michal
PY  - 2026
TI  - Integrative taxonomy reveals Parakaliella harimensis (Pilsbry, 1901) as a distinct East Asian Euconulus species with deep genetic structure
JF  - Molecular Phylogenetics and Evolution
VL  - 214
IS  - January
SP  - "108460"
EP  - "108460"
PB  - Elsevier
SN  - 10557903
KW  - Distribution
KW  - Integrative taxonomy
KW  - Land snails
KW  - New markers
KW  - Phylogeny
KW  - Stability
UR  - https://doi.org/10.1016/j.ympev.2025.108460
N2  - The remarkable phenotypic plasticity of land snail shells often results in convergent evolution, leading to frequent taxonomic misidentifications and non-monophyletic classifications. The taxonomy of the Holarctic micro land snails related to Euconulus fulvus has been particularly challenging to resolve. This study integrates mitochondrial and nuclear DNA phylogenetics, geometric morphometrics, and climate suitability modeling to clarify the phylogenetic and taxonomic status of an East Asian lineage within this group. We confirm that this lineage represents a distinct and previously misclassified species, Euconulus harimensis, which was formerly placed within the genus Parakaliella. While standard mtDNA and nDNA markers showed this evolutionary lineage as polyphyletic, two newly developed nuclear markers (ZN507 and TEP1) resolved the lineage, except for one individual, within a single robust clade of E. harimensis. Geometric morphometric analyses revealed significant differences in shell shape, which, along with unique shell microsculpture, further support its taxonomic separation. Although E. harimensis possesses the smallest distribution range of all related species, it exhibits the highest intraspecific genetic diversity. The models of suitable climatic conditions for both the present and the Last Glacial Maximum showed no major shifts, suggesting high climatic stability of the species range during the Pleistocene. This, along with other palaeobiogeographic factors, may have contributed to the unexpectedly high intraspecific genetic diversity of this species despite its limited range. Our findings provide new insights into the evolutionary processes that shape land snail inter- and intraspecific diversity, with implications for their taxonomy, which has often relied on shell morphology only. Additionally, phylogenetic analysis based on the new markers sheds novel light on the taxonomy and evolution of E. fulvus and E. alderi, highlighting the need for further taxonomic research.
ER  -

KAFIMOLA, Sara; Veronika HORSÁKOVÁ; Markéta NOVÁKOVÁ; Eva MIKULÁŠKOVÁ; Kazuki KIMURA; Stefan MENG; Jan DIVÍŠEK; Jeffrey Clark NEKOLA a Michal HORSÁK. Integrative taxonomy reveals Parakaliella harimensis (Pilsbry, 1901) as a distinct East Asian Euconulus species with deep genetic structure. \textit{Molecular Phylogenetics and Evolution}. Elsevier, 2026, roč.~214, January, s.~''108460'', 11 s. ISSN~1055-7903. Dostupné z: https://doi.org/10.1016/j.ympev.2025.108460.

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