Cytological and Immunocytochemistry Findings in Fallopian Tube
Brush Specimens and Their Correlation With Histology

J 2025

Cytological and Immunocytochemistry Findings in Fallopian Tube Brush Specimens and Their Correlation With Histology

HAUSNEROVÁ, Jitka; Robert SLUNECKO; Petra OVESNÁ; Markéta BEDNAŘÍKOVÁ; Lenka ZLÁMALÍKOVÁ et al.

Základní údaje

Originální název

Cytological and Immunocytochemistry Findings in Fallopian Tube Brush Specimens and Their Correlation With Histology

Autoři

Vydání

CYTOPATHOLOGY, HOBOKEN, WILEY, 2025, 0956-5507

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30109 Pathology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.100 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00142198

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

brush cytology; fallopian tube; high-grade serous carcinoma; immunocytochemistry

Štítky

14110212, 14110230, 14110240, 14119612, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 10. 2025 14:24, Mgr. Tereza Miškechová

Anotace

V originále

Context High-grade serous carcinoma (HGSC), the most prevalent and lethal form of ovarian cancer, is increasingly recognised to originate in the fimbrial end of the fallopian tube (FT). Timely detection remains a critical unmet clinical need due to ineffective screening methods. This prospective observational study assesses the diagnostic potential of FT brush cytology by correlating cytomorphological and immunocytochemical findings with histologically confirmed HGSC.Methods and Material A total of 134 FT from 89 patients undergoing salpingo-oophorectomy (with or without hysterectomy) were analysed. Liquid-based cytology samples were evaluated for morphological abnormalities and subjected to immunocytochemistry using p53 and Ki-67 markers. Cytological results were classified as benign, suspicious or malignant. Statistical analyses included sensitivity, specificity and odds ratio calculations via logistic regression (alpha = 0.05), performed using the R software.Results Histopathology confirmed HGSC in 15 patients. Of these, brush cytology identified 12 as suspicious or malignant, demonstrating high diagnostic concordance. Aberrant p53 expression was found in 11 cases, and a high Ki-67 proliferation index was observed in 10. These findings underscore the strong correlation between cytological, immunocytochemical and histological features of tubal HGSC.Conclusions In conclusion, FT brush cytology combined with p53 and Ki-67 immunocytochemistry shows promise as a minimally invasive approach for early HGSC detection. Future research should focus on larger prospective cohorts, ideally incorporating in vivo hysteroscopic sampling.

Citovat

HAUSNEROVÁ, Jitka; Robert SLUNECKO; Petra OVESNÁ; Markéta BEDNAŘÍKOVÁ; Lenka ZLÁMALÍKOVÁ; Jan KOSTUN; Petr STRANIK; Vendula SMOLIGOVA; Libor VIKTORA; Michaela KOBLIZKOVA; Jiri PRESL a Vít WEINBERGER. Cytological and Immunocytochemistry Findings in Fallopian Tube Brush Specimens and Their Correlation With Histology. CYTOPATHOLOGY. HOBOKEN: WILEY, 2025, roč. 36, č. 6, s. 578-588. ISSN 0956-5507. Dostupné z: https://doi.org/10.1111/cyt.70022.

@article{2523160,
   author = {Hausnerová, Jitka and Slunecko, Robert and Ovesná, Petra and Bednaříková, Markéta and Zlámalíková, Lenka and Kostun, Jan and Stranik, Petr and Smoligova, Vendula and Viktora, Libor and Koblizkova, Michaela and Presl, Jiri and Weinberger, Vít},
   article_location = {HOBOKEN},
   article_number = {6},
   doi = {https://doi.org/10.1111/cyt.70022},
   keywords = {brush cytology; fallopian tube; high-grade serous carcinoma; immunocytochemistry},
   language = {eng},
   issn = {0956-5507},
   journal = {CYTOPATHOLOGY},
   title = {Cytological and Immunocytochemistry Findings in Fallopian Tube Brush Specimens and Their Correlation With Histology},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/cyt.70022},
   volume = {36},
   year = {2025}
}

TY  - JOUR
ID  - 2523160
AU  - Hausnerová, Jitka - Slunecko, Robert - Ovesná, Petra - Bednaříková, Markéta - Zlámalíková, Lenka - Kostun, Jan - Stranik, Petr - Smoligova, Vendula - Viktora, Libor - Koblizkova, Michaela - Presl, Jiri - Weinberger, Vít
PY  - 2025
TI  - Cytological and Immunocytochemistry Findings in Fallopian Tube Brush Specimens and Their Correlation With Histology
JF  - CYTOPATHOLOGY
VL  - 36
IS  - 6
SP  - 578-588
EP  - 578-588
PB  - WILEY
SN  - 09565507
KW  - brush cytology
KW  - fallopian tube
KW  - high-grade serous carcinoma
KW  - immunocytochemistry
UR  - https://onlinelibrary.wiley.com/doi/10.1111/cyt.70022
N2  - Context High-grade serous carcinoma (HGSC), the most prevalent and lethal form of ovarian cancer, is increasingly recognised to originate in the fimbrial end of the fallopian tube (FT). Timely detection remains a critical unmet clinical need due to ineffective screening methods. This prospective observational study assesses the diagnostic potential of FT brush cytology by correlating cytomorphological and immunocytochemical findings with histologically confirmed HGSC.Methods and Material A total of 134 FT from 89 patients undergoing salpingo-oophorectomy (with or without hysterectomy) were analysed. Liquid-based cytology samples were evaluated for morphological abnormalities and subjected to immunocytochemistry using p53 and Ki-67 markers. Cytological results were classified as benign, suspicious or malignant. Statistical analyses included sensitivity, specificity and odds ratio calculations via logistic regression (alpha = 0.05), performed using the R software.Results Histopathology confirmed HGSC in 15 patients. Of these, brush cytology identified 12 as suspicious or malignant, demonstrating high diagnostic concordance. Aberrant p53 expression was found in 11 cases, and a high Ki-67 proliferation index was observed in 10. These findings underscore the strong correlation between cytological, immunocytochemical and histological features of tubal HGSC.Conclusions In conclusion, FT brush cytology combined with p53 and Ki-67 immunocytochemistry shows promise as a minimally invasive approach for early HGSC detection. Future research should focus on larger prospective cohorts, ideally incorporating in vivo hysteroscopic sampling.
ER  -

HAUSNEROVÁ, Jitka; Robert SLUNECKO; Petra OVESNÁ; Markéta BEDNAŘÍKOVÁ; Lenka ZLÁMALÍKOVÁ; Jan KOSTUN; Petr STRANIK; Vendula SMOLIGOVA; Libor VIKTORA; Michaela KOBLIZKOVA; Jiri PRESL a Vít WEINBERGER. Cytological and Immunocytochemistry Findings in Fallopian Tube Brush Specimens and Their Correlation With Histology. \textit{CYTOPATHOLOGY}. HOBOKEN: WILEY, 2025, roč.~36, č.~6, s.~578-588. ISSN~0956-5507. Dostupné z: https://doi.org/10.1111/cyt.70022.

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