Cytokinins control secondary cell wall formation in the
inflorescence stem of Arabidopsis

J 2025

Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis

DIDI, Vojtěch; Dominique ARNAUD; Alesia MELNIKAVA; Anna PAČÍNKOVÁ; Radek JUPA et al.

Základní údaje

Originální název

Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis

Autoři

Vydání

DEVELOPMENT, COMPANY BIOLOGISTS LTD, 2025, 0950-1991

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.600 v roce 2024

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1242/dev.205000

EID Scopus

2-s2.0-105016422571

Klíčová slova anglicky

Arabidopsis Cytokinins; Interfascicular fibers; Multistep phosphorelay; NAC TFs; Tracheary elements

Štítky

14314010, 143160, 143180

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 10. 2025 11:29, Mgr. Eva Dubská

Anotace

V originále

Spatiotemporal control over developmental programs is vital to all organisms. Here, we show that deficiency in cytokinin signaling or biosynthesis leads to early secondary cell wall (SCW) formation in Arabidopsis inflorescence stem that associates with precocious upregulation of an SCW transcriptional cascade controlled by NAC transcription factors (NSTs/VNDs). Cytokinin signaling through AHK2/3 and the ARR1/10/12 suppresses the expression of several NST genes and SCW formation. Exogenous cytokinin application led to fast downregulation of NST1 and NST3 (NST1/3) in the wild type and reconstituted both proper development and the apical-basal gradient of NST1/3 expression in a cytokinin-deficient mutant. AHK2 and AHK3 require functional NST1 or NST3 to control SCW initiation in the interfascicular fibers, demonstrating that cytokinins act upstream of NSTs. The premature onset of a rigid SCW biosynthesis and altered expression of NSTs due to cytokinin deficiency led to the formation of smaller tracheary elements and impaired hydraulic conductivity. We conclude that cytokinins downregulate NST genes to inhibit premature SCW formation in the apical part of the inflorescence stem, facilitating thus the development of fully functional tracheary elements and interfascicular fibers. This publication has an author's peer-reviewed open-access version available at https://doi.org/10.5817/IS.MUNI.CZ2025-2522180

Návaznosti

EF15_003/0000469, projekt VaV

Název: Cetocoen Plus

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

LM2023069, projekt VaV

Název: Výzkumná infrastruktura RECETOX

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Citovat

DIDI, Vojtěch; Dominique ARNAUD; Alesia MELNIKAVA; Anna PAČÍNKOVÁ; Radek JUPA; Radim CEGAN; Jana VAŠÍČKOVÁ; Mariana BENITEZ KEINRAD; Faride UNDA; Tereza DOBISOVÁ; Willi Sascha RIBER; Zuzana DOSTÁLOVÁ; Ondrej NOVAK; Miroslav STRNAD; Roman HOBZA; Shawn D MANSFIELD; Vít GLOSER; Eva BUDINSKÁ a Jan HEJÁTKO. Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis. DEVELOPMENT. COMPANY BIOLOGISTS LTD, 2025, roč. 152, č. 18, s. 1-21. ISSN 0950-1991. Dostupné z: https://doi.org/10.1242/dev.205000.

@article{2524423,
   author = {Didi, Vojtěch and Arnaud, Dominique and Melnikava, Alesia and Pačínková, Anna and Jupa, Radek and Cegan, Radim and Vašíčková, Jana and Benitez Keinrad, Mariana and Unda, Faride and Dobisová, Tereza and Riber, Willi Sascha and Dostálová, Zuzana and Novak, Ondrej and Strnad, Miroslav and Hobza, Roman and Mansfield, Shawn D and Gloser, Vít and Budinská, Eva and Hejátko, Jan},
   article_number = {18},
   doi = {https://doi.org/10.1242/dev.205000},
   keywords = {Arabidopsis Cytokinins; Interfascicular fibers; Multistep phosphorelay; NAC TFs; Tracheary elements},
   language = {eng},
   issn = {0950-1991},
   journal = {DEVELOPMENT},
   note = {This publication has an author's peer-reviewed open-access version available at https://doi.org/10.5817/IS.MUNI.CZ2025-2522180},
   title = {Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis},
   url = {https://journals.biologists.com/dev/article-abstract/152/18/dev205000/369180/Cytokinins-control-secondary-cell-wall-formation?redirectedFrom=fulltext},
   volume = {152},
   year = {2025}
}

TY  - JOUR
ID  - 2524423
AU  - Didi, Vojtěch - Arnaud, Dominique - Melnikava, Alesia - Pačínková, Anna - Jupa, Radek - Cegan, Radim - Vašíčková, Jana - Benitez Keinrad, Mariana - Unda, Faride - Dobisová, Tereza - Riber, Willi Sascha - Dostálová, Zuzana - Novak, Ondrej - Strnad, Miroslav - Hobza, Roman - Mansfield, Shawn D - Gloser, Vít - Budinská, Eva - Hejátko, Jan
PY  - 2025
TI  - Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis
JF  - DEVELOPMENT
VL  - 152
IS  - 18
SP  - 1-21
EP  - 1-21
PB  - COMPANY BIOLOGISTS LTD
SN  - 09501991
N1  - This publication has an author's peer-reviewed open-access version available at https://doi.org/10.5817/IS.MUNI.CZ2025-2522180
KW  - Arabidopsis Cytokinins
KW  - Interfascicular fibers
KW  - Multistep phosphorelay
KW  - NAC TFs
KW  - Tracheary elements
UR  - https://journals.biologists.com/dev/article-abstract/152/18/dev205000/369180/Cytokinins-control-secondary-cell-wall-formation?redirectedFrom=fulltext
N2  - Spatiotemporal control over developmental programs is vital to all organisms. Here, we show that deficiency in cytokinin signaling or biosynthesis leads to early secondary cell wall (SCW) formation in Arabidopsis inflorescence stem that associates with precocious upregulation of an SCW transcriptional cascade controlled by NAC transcription factors (NSTs/VNDs). Cytokinin signaling through AHK2/3 and the ARR1/10/12 suppresses the expression of several NST genes and SCW formation. Exogenous cytokinin application led to fast downregulation of NST1 and NST3 (NST1/3) in the wild type and reconstituted both proper development and the apical-basal gradient of NST1/3 expression in a cytokinin-deficient mutant. AHK2 and AHK3 require functional NST1 or NST3 to control SCW initiation in the interfascicular fibers, demonstrating that cytokinins act upstream of NSTs. The premature onset of a rigid SCW biosynthesis and altered expression of NSTs due to cytokinin deficiency led to the formation of smaller tracheary elements and impaired hydraulic conductivity. We conclude that cytokinins downregulate NST genes to inhibit premature SCW formation in the apical part of the inflorescence stem, facilitating thus the development of fully functional tracheary elements and interfascicular fibers. This publication has an author's peer-reviewed open-access version available at https://doi.org/10.5817/IS.MUNI.CZ2025-2522180
ER  -

DIDI, Vojtěch; Dominique ARNAUD; Alesia MELNIKAVA; Anna PAČÍNKOVÁ; Radek JUPA; Radim CEGAN; Jana VAŠÍČKOVÁ; Mariana BENITEZ KEINRAD; Faride UNDA; Tereza DOBISOVÁ; Willi Sascha RIBER; Zuzana DOSTÁLOVÁ; Ondrej NOVAK; Miroslav STRNAD; Roman HOBZA; Shawn D MANSFIELD; Vít GLOSER; Eva BUDINSKÁ a Jan HEJÁTKO. Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis. \textit{DEVELOPMENT}. COMPANY BIOLOGISTS LTD, 2025, roč.~152, č.~18, s.~1-21. ISSN~0950-1991. Dostupné z: https://doi.org/10.1242/dev.205000.

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