A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids
In Situ Reveals Berberine-Stabilized Hoogsteen Structures in
Mitochondria

J 2025

A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria

DZUROV KRAFČÍKOVÁ, Michaela; David BERIASHVILI; Salima BAHRI; Menno BERGMEIJER; Stuart C. HOWES et al.

Základní údaje

Originální název

A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria

Autoři

DZUROV KRAFČÍKOVÁ, Michaela ; David BERIASHVILI; Salima BAHRI; Menno BERGMEIJER; Stuart C. HOWES; Andrei GURINOV; Friedrich G. FORSTER; Gert E. FOLKERS a Marc BALDUS

Vydání

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, WEINHEIM, WILEY-V C H VERLAG GMBH, 2025

Další údaje

Typ výsledku

Článek v odborném periodiku

Označené pro přenos do RIV

DOI

https://doi.org/10.1002/anie.202424131

UT WoS

001446775300001

Klíčová slova anglicky

Berberine; DNA/RNA; DNP-ssNMR; G-quadruplex; Mitochondria

Změněno: 20. 1. 2026 15:10, Mgr. Michaela Dzurov Krafčíková, Ph.D.

Anotace

V originále

Mitochondria are central to cellular bioenergetics, with the unique ability to translate and transcribe a subset of their own proteome. Given the critical importance of energy production, mitochondria seem to utilize higher-order nucleic acid structures to regulate gene expression, much like nuclei. Herein, we introduce a tailored approach to probe the formation of such structures, specifically G-quadruplexes, within intact mitochondria by using sensitivity-enhanced dynamic nuclear polarization-supported solid-state NMR (DNP-ssNMR). We acquired NMR spectra on isolated intact isotopically labeled mitochondria treated with berberine, a known high-affinity G-quadruplex stabilizer. The DNP-ssNMR data revealed spectral changes in nucleic acid sugar correlations, increased signal intensity for guanosine carbons, and enhanced Hoogsteen hydrogen bond formation, providing evidence of in vivo G-quadruplex formation in mitochondria. Together, our workflow enables the study of mitochondrial nucleic acid-ligand interactions at endogenous concentrations within biologically relevant environments by DNP-ssNMR, thus paving the way for future research into mitochondrial diseases and their potential treatments.

Citovat

DZUROV KRAFČÍKOVÁ, Michaela; David BERIASHVILI; Salima BAHRI; Menno BERGMEIJER; Stuart C. HOWES; Andrei GURINOV; Friedrich G. FORSTER; Gert E. FOLKERS a Marc BALDUS. A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria. Online. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. WEINHEIM: WILEY-V C H VERLAG GMBH, 2025, roč. 64, č. 21, s. 1-9. Dostupné z: https://doi.org/10.1002/anie.202424131.

@article{2526339,
   author = {Dzurov Krafčíková, Michaela and Beriashvili, David and Bahri, Salima and Bergmeijer, Menno and Howes, Stuart C. and Gurinov, Andrei and Forster, Friedrich G. and Folkers, Gert E. and Baldus, Marc},
   article_location = {WEINHEIM},
   article_number = {21},
   doi = {https://doi.org/10.1002/anie.202424131},
   keywords = {Berberine; DNA/RNA; DNP-ssNMR; G-quadruplex; Mitochondria},
   journal = {ANGEWANDTE CHEMIE-INTERNATIONAL EDITION},
   title = {A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria},
   volume = {64},
   year = {2025}
}

TY  - JOUR
ID  - 2526339
AU  - Dzurov Krafčíková, Michaela - Beriashvili, David - Bahri, Salima - Bergmeijer, Menno - Howes, Stuart C. - Gurinov, Andrei - Forster, Friedrich G. - Folkers, Gert E. - Baldus, Marc
PY  - 2025
TI  - A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria
JF  - ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
VL  - 64
IS  - 21
SP  - 1-9
EP  - 1-9
PB  - WILEY-V C H VERLAG GMBH
KW  - Berberine
KW  - DNA/RNA
KW  - DNP-ssNMR
KW  - G-quadruplex
KW  - Mitochondria
N2  - Mitochondria are central to cellular bioenergetics, with the unique ability to translate and transcribe a subset of their own proteome. Given the critical importance of energy production, mitochondria seem to utilize higher-order nucleic acid structures to regulate gene expression, much like nuclei. Herein, we introduce a tailored approach to probe the formation of such structures, specifically G-quadruplexes, within intact mitochondria by using sensitivity-enhanced dynamic nuclear polarization-supported solid-state NMR (DNP-ssNMR). We acquired NMR spectra on isolated intact isotopically labeled mitochondria treated with berberine, a known high-affinity G-quadruplex stabilizer. The DNP-ssNMR data revealed spectral changes in nucleic acid sugar correlations, increased signal intensity for guanosine carbons, and enhanced Hoogsteen hydrogen bond formation, providing evidence of in vivo G-quadruplex formation in mitochondria. Together, our workflow enables the study of mitochondrial nucleic acid-ligand interactions at endogenous concentrations within biologically relevant environments by DNP-ssNMR, thus paving the way for future research into mitochondrial diseases and their potential treatments.
ER  -

DZUROV KRAFČÍKOVÁ, Michaela; David BERIASHVILI; Salima BAHRI; Menno BERGMEIJER; Stuart C. HOWES; Andrei GURINOV; Friedrich G. FORSTER; Gert E. FOLKERS a Marc BALDUS. A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria. Online. \textit{ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2025, roč.~64, č.~21, s.~1-9. Dostupné z: https://doi.org/10.1002/anie.202424131.

Přehled o publikaci