Detection and quantification of the canine DNA in meat DNA
mixes and meat mixtures by real time PCR and digital droplet
PCR

J 2025

Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR

NESVADBOVA, Michaela; Radka DZIEDZINSKA; Vladimir BABAK a Petr KRÁLÍK

Základní údaje

Originální název

Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR

Autoři

NESVADBOVA, Michaela; Radka DZIEDZINSKA; Vladimir BABAK a Petr KRÁLÍK

Vydání

Journal of Food Composition and Analysis, Elsevier, 2025, 0889-1575

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21101 Food and beverages

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00142388

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

qPCR; Quantification; Meat species content

Štítky

14313050, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 10. 2025 10:55, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Accurate detection of animal species in meat products is essential for food safety, regulatory compliance, and the prevention of food fraud. This study presents the development and validation of a modified quadruplex quantitative PCR (qPCR) assay and singleplex droplet digital PCR (ddPCR) for the detection and quantification of canine DNA in meat and DNA mixtures. Two- and three-species samples containing canine, pork, and chicken components (0.1 %-100 %) were prepared, including heat-treated variants. A simplified quantification approach using a single 100 % DNA reference diluted to 10 ng/mu L was applied in both methods. Results from qPCR and ddPCR were statistically evaluated using ANOVA, RMSD, and coefficient of variation (CV). Both methods yielded accurate results across most concentration ranges, with no significant differences in calculated percentages. However, qPCR exhibited significantly higher variability than ddPCR, especially at extreme concentrations (<1 % and >99 %). ddPCR showed superior performance in three-species mixtures and yielded lower RMSD values overall. Despite higher variability at detection limits inherent to PCR technology, both methods are suitable for routine quantification of canine DNA in meat products. The study confirms that a single-reference standard is a cost-effective and reliable alternative to full calibration curves for species quantification.

Citovat

NESVADBOVA, Michaela; Radka DZIEDZINSKA; Vladimir BABAK a Petr KRÁLÍK. Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR. Journal of Food Composition and Analysis. Elsevier, 2025, roč. 146, October, s. 107855-107861. ISSN 0889-1575. Dostupné z: https://doi.org/10.1016/j.jfca.2025.107855.

@article{2529121,
   author = {Nesvadbova, Michaela and Dziedzinska, Radka and Babak, Vladimir and Králík, Petr},
   article_number = {October},
   doi = {https://doi.org/10.1016/j.jfca.2025.107855},
   keywords = {qPCR; Quantification; Meat species content},
   language = {eng},
   issn = {0889-1575},
   journal = {Journal of Food Composition and Analysis},
   title = {Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR},
   url = {https://doi.org/10.1016/j.jfca.2025.107855},
   volume = {146},
   year = {2025}
}

TY  - JOUR
ID  - 2529121
AU  - Nesvadbova, Michaela - Dziedzinska, Radka - Babak, Vladimir - Králík, Petr
PY  - 2025
TI  - Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR
JF  - Journal of Food Composition and Analysis
VL  - 146
IS  - October
SP  - 107855
EP  - 107855
PB  - Elsevier
SN  - 08891575
KW  - qPCR
KW  - Quantification
KW  - Meat species content
UR  - https://doi.org/10.1016/j.jfca.2025.107855
N2  - Accurate detection of animal species in meat products is essential for food safety, regulatory compliance, and the prevention of food fraud. This study presents the development and validation of a modified quadruplex quantitative PCR (qPCR) assay and singleplex droplet digital PCR (ddPCR) for the detection and quantification of canine DNA in meat and DNA mixtures. Two- and three-species samples containing canine, pork, and chicken components (0.1 %-100 %) were prepared, including heat-treated variants. A simplified quantification approach using a single 100 % DNA reference diluted to 10 ng/mu L was applied in both methods. Results from qPCR and ddPCR were statistically evaluated using ANOVA, RMSD, and coefficient of variation (CV). Both methods yielded accurate results across most concentration ranges, with no significant differences in calculated percentages. However, qPCR exhibited significantly higher variability than ddPCR, especially at extreme concentrations (<1 % and >99 %). ddPCR showed superior performance in three-species mixtures and yielded lower RMSD values overall. Despite higher variability at detection limits inherent to PCR technology, both methods are suitable for routine quantification of canine DNA in meat products. The study confirms that a single-reference standard is a cost-effective and reliable alternative to full calibration curves for species quantification.
ER  -

NESVADBOVA, Michaela; Radka DZIEDZINSKA; Vladimir BABAK a Petr KRÁLÍK. Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR. \textit{Journal of Food Composition and Analysis}. Elsevier, 2025, roč.~146, October, s.~107855-107861. ISSN~0889-1575. Dostupné z: https://doi.org/10.1016/j.jfca.2025.107855.

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