Engineering Two-in-One Nanoparticles for Simultaneous Delivery
of Graphene Quantum Dot and Pemetrexed

J 2025

Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed

OZ, Umut Can; Berrin KUCUKTURKMEN; I Jennifer GOMEZ; Amr ELSHERBENY; Seda Ipek TEKNECI et al.

Základní údaje

Originální název

Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed

Autoři

Vydání

ACS Omega, American Chemical Society, 2025, 2470-1343

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00142461

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cancer; Drug delivery; Hydrodynamics; Nanoparticles; Quantum dots

Štítky

14312020, 14314010, CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 3. 2026 10:02, Mgr. Eva Dubská

Anotace

V originále

The simultaneous delivery of therapeutic agents and imaging probes using polymeric nanoparticles (NPs) has gained significant attention for cancer treatment. In this work, we developed a multifunctional nanocarrier system composed of an amphiphilic block copolymer, poly(2-ethyl-2-oxazoline)-b-poly(ε-caprolactone) (PEtOx-b-PCL), and dimethyldidodecylammonium bromide (DDAB), for the codelivery of the chemotherapeutic drug pemetrexed (PMT) and nitrogen- or sulfur-doped graphene quantum dots (N-GQDs or S-GQDs) as fluorescent probes. Critical formulation parameters were optimized using a central composite design (CCD). The optimized NPs exhibited favorable physicochemical properties, including positive surface charge (6–8 mV), hydrodynamic diameters of ∼140 nm, and high encapsulation efficiency for both PMT (46–56%) and GQDs (>98%). In vitro assays revealed that PMT-loaded nanoparticles (NPs) significantly enhanced cytotoxicity against MCF-7 cells. At a concentration of 2 ppm after 72 h, N-PMT NPs and S-PMT NPs inhibited cell proliferation by 50.7% and 53.8%, respectively, compared to 37.8% inhibition with free PMT at the same dose. Confocal microscopy confirmed efficient intracellular uptake and strong fluorescence signals, supporting their potential for bioimaging. Collectively, these results demonstrate that this two-in-one nanocarrier system significantly enhances chemotherapeutic efficacy while enabling real-time imaging, establishing a promising platform for drug delivery and noninvasive treatment monitoring in cancer nanomedicine.

Návaznosti

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

OZ, Umut Can; Berrin KUCUKTURKMEN; I Jennifer GOMEZ; Amr ELSHERBENY; Seda Ipek TEKNECI; Ozgur ESIM; Selin GOKSEVER; Umut Ugur OZKOSE; Sevgi GULYUZ; Claudia BAZAN-COBELO; Ozgur YILMAZ; Aylin USTUNDAG; Jiřina MEDALOVÁ; Asuman BOZKIR; Lenka ZAJÍČKOVÁ a Engin ER. Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed. ACS Omega. American Chemical Society, 2025, roč. 10, č. 41, s. 48934-48947. ISSN 2470-1343. Dostupné z: https://doi.org/10.1021/acsomega.5c07253.

@article{2530803,
   author = {Oz, Umut Can and Kucukturkmen, Berrin and Gomez, I Jennifer and Elsherbeny, Amr and Tekneci, Seda Ipek and Esim, Ozgur and Goksever, Selin and Ozkose, Umut Ugur and Gulyuz, Sevgi and BazanandCobelo, Claudia and Yilmaz, Ozgur and Ustundag, Aylin and Medalová, Jiřina and Bozkir, Asuman and Zajíčková, Lenka and Er, Engin},
   article_number = {41},
   doi = {https://doi.org/10.1021/acsomega.5c07253},
   keywords = {Cancer; Drug delivery; Hydrodynamics; Nanoparticles; Quantum dots},
   language = {eng},
   issn = {2470-1343},
   journal = {ACS Omega},
   title = {Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed},
   url = {https://pubs.acs.org/doi/10.1021/acsomega.5c07253?ref=PDF},
   volume = {10},
   year = {2025}
}

TY  - JOUR
ID  - 2530803
AU  - Oz, Umut Can - Kucukturkmen, Berrin - Gomez, I Jennifer - Elsherbeny, Amr - Tekneci, Seda Ipek - Esim, Ozgur - Goksever, Selin - Ozkose, Umut Ugur - Gulyuz, Sevgi - Bazan-Cobelo, Claudia - Yilmaz, Ozgur - Ustundag, Aylin - Medalová, Jiřina - Bozkir, Asuman - Zajíčková, Lenka - Er, Engin
PY  - 2025
TI  - Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed
JF  - ACS Omega
VL  - 10
IS  - 41
SP  - 48934-48947
EP  - 48934-48947
PB  - American Chemical Society
SN  - 24701343
KW  - Cancer
KW  - Drug delivery
KW  - Hydrodynamics
KW  - Nanoparticles
KW  - Quantum dots
UR  - https://pubs.acs.org/doi/10.1021/acsomega.5c07253?ref=PDF
N2  - The simultaneous delivery of therapeutic agents and imaging probes using polymeric nanoparticles (NPs) has gained significant attention for cancer treatment. In this work, we developed a multifunctional nanocarrier system composed of an amphiphilic block copolymer, poly(2-ethyl-2-oxazoline)-b-poly(ε-caprolactone) (PEtOx-b-PCL), and dimethyldidodecylammonium bromide (DDAB), for the codelivery of the chemotherapeutic drug pemetrexed (PMT) and nitrogen- or sulfur-doped graphene quantum dots (N-GQDs or S-GQDs) as fluorescent probes. Critical formulation parameters were optimized using a central composite design (CCD). The optimized NPs exhibited favorable physicochemical properties, including positive surface charge (6–8 mV), hydrodynamic diameters of ∼140 nm, and high encapsulation efficiency for both PMT (46–56%) and GQDs (>98%). In vitro assays revealed that PMT-loaded nanoparticles (NPs) significantly enhanced cytotoxicity against MCF-7 cells. At a concentration of 2 ppm after 72 h, N-PMT NPs and S-PMT NPs inhibited cell proliferation by 50.7% and 53.8%, respectively, compared to 37.8% inhibition with free PMT at the same dose. Confocal microscopy confirmed efficient intracellular uptake and strong fluorescence signals, supporting their potential for bioimaging. Collectively, these results demonstrate that this two-in-one nanocarrier system significantly enhances chemotherapeutic efficacy while enabling real-time imaging, establishing a promising platform for drug delivery and noninvasive treatment monitoring in cancer nanomedicine.
ER  -

OZ, Umut Can; Berrin KUCUKTURKMEN; I Jennifer GOMEZ; Amr ELSHERBENY; Seda Ipek TEKNECI; Ozgur ESIM; Selin GOKSEVER; Umut Ugur OZKOSE; Sevgi GULYUZ; Claudia BAZAN-COBELO; Ozgur YILMAZ; Aylin USTUNDAG; Jiřina MEDALOVÁ; Asuman BOZKIR; Lenka ZAJÍČKOVÁ a Engin ER. Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed. \textit{ACS Omega}. American Chemical Society, 2025, roč.~10, č.~41, s.~48934-48947. ISSN~2470-1343. Dostupné z: https://doi.org/10.1021/acsomega.5c07253.

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