Characterising the Periodontal Granulation Tissue Using
scRNAseq

J 2026

Characterising the Periodontal Granulation Tissue Using scRNAseq

ZHU, Wentao; Kathy FUNG; Pawan DHAMI; Paul SHARPE; Jan KŘIVÁNEK et al.

Základní údaje

Originální název

Characterising the Periodontal Granulation Tissue Using scRNAseq

Autoři

ZHU, Wentao; Kathy FUNG; Pawan DHAMI; Paul SHARPE; Jan KŘIVÁNEK; Luigi NIBALI; Cheng ZHANG a Vitor C M NEVES

Vydání

Journal of Clinical Periodontology, Hoboken, Wiley-Blackwell, 2026, 0303-6979

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.800 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

periodontal granulation tissue; single-cell RNA sequencing; cellular heterogeneity; periodontal inflammation; tissue microenvironment

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 2. 2026 12:24, Mgr. Tereza Miškechová

Anotace

V originále

Aim To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single-cell RNA sequencing (scRNA-seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.Materials and Methods Granulation tissue samples were collected from patients undergoing periodontal surgery (n = 3). Fresh tissues were processed into single-cell suspensions and subjected to scRNA-seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.Results Ten distinct cell clusters were identified across the samples. Granulation tissue exhibited a higher abundance of immune cells compared to healthy tissues. A novel endothelial cell subpopulation, exclusive to granulation tissue, was discovered and characterised by NOTCH3 expression and involvement in ossification pathways. Additionally, granulation tissue fibroblast subpopulations demonstrated a progenitor-like state, characterised by extracellular matrix reorganisation and low differentiation, similar to cancer-associated fibroblasts.Conclusion This study identified a novel endothelial subpopulation offering new insights into the disease's pathogenesis and presenting potential targets for regenerative therapies. These findings will help advance the understanding of periodontal disease granulation tissue formation and provide information for the development of materials to modulate specific cellular pathways to improve periodontal disease management.

Citovat

ZHU, Wentao; Kathy FUNG; Pawan DHAMI; Paul SHARPE; Jan KŘIVÁNEK; Luigi NIBALI; Cheng ZHANG a Vitor C M NEVES. Characterising the Periodontal Granulation Tissue Using scRNAseq. Journal of Clinical Periodontology. Hoboken: Wiley-Blackwell, 2026, roč. 53, č. 2, s. 308-320. ISSN 0303-6979. Dostupné z: https://doi.org/10.1111/jcpe.70048.

@article{2531001,
   author = {Zhu, Wentao and Fung, Kathy and Dhami, Pawan and Sharpe, Paul and Křivánek, Jan and Nibali, Luigi and Zhang, Cheng and Neves, Vitor C M},
   article_location = {Hoboken},
   article_number = {2},
   doi = {https://doi.org/10.1111/jcpe.70048},
   keywords = {periodontal granulation tissue; single-cell RNA sequencing; cellular heterogeneity; periodontal inflammation; tissue microenvironment},
   language = {eng},
   issn = {0303-6979},
   journal = {Journal of Clinical Periodontology},
   title = {Characterising the Periodontal Granulation Tissue Using scRNAseq},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jcpe.70048},
   volume = {53},
   year = {2026}
}

TY  - JOUR
ID  - 2531001
AU  - Zhu, Wentao - Fung, Kathy - Dhami, Pawan - Sharpe, Paul - Křivánek, Jan - Nibali, Luigi - Zhang, Cheng - Neves, Vitor C M
PY  - 2026
TI  - Characterising the Periodontal Granulation Tissue Using scRNAseq
JF  - Journal of Clinical Periodontology
VL  - 53
IS  - 2
SP  - 308-320
EP  - 308-320
PB  - Wiley-Blackwell
SN  - 03036979
KW  - periodontal granulation tissue
KW  - single-cell RNA sequencing
KW  - cellular heterogeneity
KW  - periodontal inflammation
KW  - tissue microenvironment
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/jcpe.70048
N2  - Aim To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single-cell RNA sequencing (scRNA-seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.Materials and Methods Granulation tissue samples were collected from patients undergoing periodontal surgery (n = 3). Fresh tissues were processed into single-cell suspensions and subjected to scRNA-seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.Results Ten distinct cell clusters were identified across the samples. Granulation tissue exhibited a higher abundance of immune cells compared to healthy tissues. A novel endothelial cell subpopulation, exclusive to granulation tissue, was discovered and characterised by NOTCH3 expression and involvement in ossification pathways. Additionally, granulation tissue fibroblast subpopulations demonstrated a progenitor-like state, characterised by extracellular matrix reorganisation and low differentiation, similar to cancer-associated fibroblasts.Conclusion This study identified a novel endothelial subpopulation offering new insights into the disease's pathogenesis and presenting potential targets for regenerative therapies. These findings will help advance the understanding of periodontal disease granulation tissue formation and provide information for the development of materials to modulate specific cellular pathways to improve periodontal disease management.
ER  -

ZHU, Wentao; Kathy FUNG; Pawan DHAMI; Paul SHARPE; Jan KŘIVÁNEK; Luigi NIBALI; Cheng ZHANG a Vitor C M NEVES. Characterising the Periodontal Granulation Tissue Using scRNAseq. \textit{Journal of Clinical Periodontology}. Hoboken: Wiley-Blackwell, 2026, roč.~53, č.~2, s.~308-320. ISSN~0303-6979. Dostupné z: https://doi.org/10.1111/jcpe.70048.

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