Fins as a reliable surrogate tissue for age-related changes of
telomeres and DNA methylation in gonads of a short-lived fish

J 2025

Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish

VRTÍLEK, Milan; Anna KROMEROVÁ; Malahat DIANAT; Miloslava FOJTOVÁ; Dagmar CIZKOVA et al.

Základní údaje

Originální název

Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish

Autoři

VRTÍLEK, Milan; Anna KROMEROVÁ; Malahat DIANAT; Miloslava FOJTOVÁ ; Dagmar CIZKOVA a Jiří FAJKUS

Vydání

BIOGERONTOLOGY, NEW YORK, SPRINGER, 2025, 1389-5729

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00142566

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Senescence; Telomeres; Methylation; Soma; Germline; Nothobranchius furzeri

Štítky

143160, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 11. 2025 09:28, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Senescence is a multifactorial and individualised process of age-related physiological decline. Cellular markers, such as telomere length and DNA methylation, can reveal subtle changes associated with chronological age or expected lifespan. In this study, we evaluated the utility of fin tissue as a surrogate for assessing telomere length and proportion of DNA methylation in the gonads of a small, short-lived laboratory fish, the turquoise killifish (Nothobranchius furzeri). We collected fin and gonadal tissues from both females and males at three different ages. We extracted DNA to measure telomere length via terminal restriction fragment (TRF) analysis and global DNA methylation levels using double-digest restriction-associated DNA sequencing (ddRADseq). Our results show a notable correspondence between telomere length and DNA methylation patterns in fin and gonadal tissues. These findings support the use of fin biopsies as a non-lethal method for assessing ageing biomarkers in the gonads of small freshwater fish.

Návaznosti

GA22-21198S, projekt VaV

Název: Mechanismy vlivu rodičovského stárnutí na potomstvo u krátkověkého obratlovce

Investor: Grantová agentura ČR, Mechanismy vlivu rodičovského stárnutí na potomstvo u krátkověkého obratlovce

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

VRTÍLEK, Milan; Anna KROMEROVÁ; Malahat DIANAT; Miloslava FOJTOVÁ; Dagmar CIZKOVA a Jiří FAJKUS. Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish. BIOGERONTOLOGY. NEW YORK: SPRINGER, 2025, roč. 26, č. 6, s. 1-15. ISSN 1389-5729. Dostupné z: https://doi.org/10.1007/s10522-025-10338-4.

@article{2532620,
   author = {Vrtílek, Milan and Kromerová, Anna and Dianat, Malahat and Fojtová, Miloslava and Cizkova, Dagmar and Fajkus, Jiří},
   article_location = {NEW YORK},
   article_number = {6},
   doi = {https://doi.org/10.1007/s10522-025-10338-4},
   keywords = {Senescence; Telomeres; Methylation; Soma; Germline; Nothobranchius furzeri},
   language = {eng},
   issn = {1389-5729},
   journal = {BIOGERONTOLOGY},
   title = {Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish},
   url = {https://doi.org/10.1007/s10522-025-10338-4},
   volume = {26},
   year = {2025}
}

TY  - JOUR
ID  - 2532620
AU  - Vrtílek, Milan - Kromerová, Anna - Dianat, Malahat - Fojtová, Miloslava - Cizkova, Dagmar - Fajkus, Jiří
PY  - 2025
TI  - Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish
JF  - BIOGERONTOLOGY
VL  - 26
IS  - 6
SP  - 1-15
EP  - 1-15
PB  - SPRINGER
SN  - 13895729
KW  - Senescence
KW  - Telomeres
KW  - Methylation
KW  - Soma
KW  - Germline
KW  - Nothobranchius furzeri
UR  - https://doi.org/10.1007/s10522-025-10338-4
N2  - Senescence is a multifactorial and individualised process of age-related physiological decline. Cellular markers, such as telomere length and DNA methylation, can reveal subtle changes associated with chronological age or expected lifespan. In this study, we evaluated the utility of fin tissue as a surrogate for assessing telomere length and proportion of DNA methylation in the gonads of a small, short-lived laboratory fish, the turquoise killifish (Nothobranchius furzeri). We collected fin and gonadal tissues from both females and males at three different ages. We extracted DNA to measure telomere length via terminal restriction fragment (TRF) analysis and global DNA methylation levels using double-digest restriction-associated DNA sequencing (ddRADseq). Our results show a notable correspondence between telomere length and DNA methylation patterns in fin and gonadal tissues. These findings support the use of fin biopsies as a non-lethal method for assessing ageing biomarkers in the gonads of small freshwater fish.
ER  -

VRTÍLEK, Milan; Anna KROMEROVÁ; Malahat DIANAT; Miloslava FOJTOVÁ; Dagmar CIZKOVA a Jiří FAJKUS. Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish. \textit{BIOGERONTOLOGY}. NEW YORK: SPRINGER, 2025, roč.~26, č.~6, s.~1-15. ISSN~1389-5729. Dostupné z: https://doi.org/10.1007/s10522-025-10338-4.

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