Novel multipurpose matrix for a high resolution MALDI MS and
multimodal imaging

k 2025

Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging

JAVOREK, Michal; Kateřina TEXLOVÁ; Michal HENDRYCH; Kateřina ONDRÁKOVÁ; Alexander POTTHOFF et al.

Základní údaje

Originální název

Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging

Autoři

JAVOREK, Michal ; Kateřina TEXLOVÁ; Michal HENDRYCH; Kateřina ONDRÁKOVÁ; Alexander POTTHOFF; Jens SOLTWISCH; Jan PREISLER; Jan FRIČ; Klaus DREISEWERD a Antonín BEDNAŘÍK

Vydání

CECE Junior 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10406 Analytical chemistry

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MALDI MSI; multimodal imaging; basic fuchsin; lipids; staining of tissue; dyes

Štítky

14313010

Změněno: 13. 11. 2025 12:39, Bc. Michal Javorek

Anotace

V originále

The staining with basic fuchsin streamlines sample preparation, eliminating the need for matrix spraying or sublimation, and enables true multimodal analysis on a single tissue section thanks to its compatibility with all employed imaging modalities. Innate fluorescence of basic fuchsin ensuresthat no additional sample treatment or addition of other fluorophores is needed for recording fluorescence images. The technique is demonstrated on mapping of mouse brain: MALDI MSI modality visualizes spatial distribution of lipids and fluorescence combined with histology clearly defines the different areas and tissue types in the brain section. Furthermore, MALDI MS with basic fuchsin is usable with laser postionization, i.e. MALDI-2, to increase sensitivity and spatial resolution to 5 micrometers. The high-quality of recorded images is comparable to the MALDI/MALDI-2 MSI with classical matrices. The developed multimodal workflow facilitates high-resolution molecular imaging and offers tools for holistic characterization of biological tissues.

Návaznosti

GA24-10924S, projekt VaV

Název: Digitální multiplexní zobrazovací hmotnostní spektrometrie

Investor: Grantová agentura ČR, Digitální multiplexní zobrazovací hmotnostní spektrometrie

MUNI/A/1559/2023, interní kód MU

Název: Výzvy v neuroonkologii: molekulární mechanismy a biomarkery

Investor: Masarykova univerzita, Výzvy v neuroonkologii: molekulární mechanismy a biomarkery

Citovat

JAVOREK, Michal; Kateřina TEXLOVÁ; Michal HENDRYCH; Kateřina ONDRÁKOVÁ; Alexander POTTHOFF; Jens SOLTWISCH; Jan PREISLER; Jan FRIČ; Klaus DREISEWERD a Antonín BEDNAŘÍK. Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging. In CECE Junior 2025. 2025.

@proceedings{2532769,
   author = {Javorek, Michal and Texlová, Kateřina and Hendrych, Michal and Ondráková, Kateřina and Potthoff, Alexander and Soltwisch, Jens and Preisler, Jan and Frič, Jan and Dreisewerd, Klaus and Bednařík, Antonín},
   booktitle = {CECE Junior 2025},
   keywords = {MALDI MSI; multimodal imaging; basic fuchsin; lipids; staining of tissue; dyes},
   language = {eng},
   title = {Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging},
   year = {2025}
}

TY  - CONF
ID  - 2532769
AU  - Javorek, Michal - Texlová, Kateřina - Hendrych, Michal - Ondráková, Kateřina - Potthoff, Alexander - Soltwisch, Jens - Preisler, Jan - Frič, Jan - Dreisewerd, Klaus - Bednařík, Antonín
PY  - 2025
TI  - Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging
KW  - MALDI MSI
KW  - multimodal imaging
KW  - basic fuchsin
KW  - lipids
KW  - staining of tissue
KW  - dyes
N2  - The staining with basic fuchsin streamlines sample preparation, eliminating the need for matrix spraying or sublimation, and enables true multimodal analysis on a single tissue section thanks to its compatibility with all employed imaging modalities. Innate fluorescence of basic fuchsin ensuresthat no additional sample treatment or addition of other fluorophores is needed for recording fluorescence images. The technique is demonstrated on mapping of mouse brain: MALDI MSI modality visualizes spatial distribution of lipids and fluorescence combined with histology clearly defines the different areas and tissue types in the brain section. Furthermore, MALDI MS with basic fuchsin is usable with laser postionization, i.e. MALDI-2, to increase sensitivity and spatial resolution to 5 micrometers. The high-quality of recorded images is comparable to the MALDI/MALDI-2 MSI with classical matrices. The developed multimodal workflow facilitates high-resolution molecular imaging and offers tools for holistic characterization of biological tissues.
ER  -

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