Studies of microbiological quality of preparations with
antimicrobial preservatives and therapeutic bacteriophages of
the genera Pbunavirus and Kayvirus

a 2025

Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus

KOMÁRKOVÁ, Marie; Martin BENEŠÍK; Tereza PROCHÁZKOVÁ; Adam VINCO; Monika LAICHMANOVÁ et al.

Základní údaje

Originální název

Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus

Autoři

KOMÁRKOVÁ, Marie ; Martin BENEŠÍK; Tereza PROCHÁZKOVÁ; Adam VINCO; Monika LAICHMANOVÁ; Soňa SMETANOVÁ; Petr JELÍNEK; Marek MOŠA; Tibor BOTKA; Miroslav ŠOÓŠ a Roman PANTŮČEK

Vydání

XXXIV. konference mladých mikrobiologů - TOMÁŠKOVY DNY 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0776-8

Klíčová slova česky

fágová terapie; Kayvirus; Pbunavirus

Klíčová slova anglicky

phage therapy; Kayvirus; Pbunavirus

Změněno: 13. 11. 2025 10:27, Mgr. Tibor Botka, Ph.D.

Anotace

V originále

Antimicrobial preservatives can be used to maintain the microbiological quality of phage preparations, but due to their potential antiviral effect, they may have an adverse influence on phage activity. Moreover, there is a possibility of physicochemical incompatibilities between phage particles and the preservatives. These phenomena may disrupt the effectiveness and quality of the final dosage form containing phages as an active ingredient. For this reason, the subject of this study is the compatibility of antimicrobial preservatives with therapeutic phages in liquid dosage form. We evaluated the stability of phage activity after exposure to 12 preservatives selected from various chemical classes by the double-layer agar method. Staphylococcal phage DSM 33473 of the genus Kayvirus and the pseudomonal phage DSM 33593 of the genus Pbunavirus, with a 9 log PFU/ml titer purified into physiological saline solution, were tested. The influence of the phages on the effect of the preservatives was determined by the microdilution method on Escherichia coli CCM 4517, Candida albicans CCM 8215 and Aspergillus brasiliensis CCM 8222. Physicochemical incompatibilities were evaluated by dynamic light scattering analysis. The assessed preservatives were classified into suitable, moderately suitable and unsuitable for manufacturing phage preparations. The suitable preservatives with slight reduction in phage titre were m-cresol, phenylethyl alcohol, sodium benzoate and sodium propionate. The unsuitable were chlorhexidine digluconate, benzalkonium chloride, thimerosal and diazolidinylurea. Chlorhexidine precipitated in the absence of phages, while benzalkonium only in the presence of phages. Thimerosal caused a decrease of 7 log PFU/ml only in DSM 33593. Diazolidinylurea led to a complete loss of titer in both genera. The result of this work is a classification of preservatives that can be used to optimise dosage forms with bacteriophages and ensure that they do not pose risks to patients due to microbial contamination or phage degradation caused by excipients. The study was supported by the National Institute of Virology and Bacteriology (LX22NPO5103) - Funded by the EU - NGEU. The students wish to thank the GA MU for its support (MUNI/A/1603/2024).

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

KOMÁRKOVÁ, Marie; Martin BENEŠÍK; Tereza PROCHÁZKOVÁ; Adam VINCO; Monika LAICHMANOVÁ; Soňa SMETANOVÁ; Petr JELÍNEK; Marek MOŠA; Tibor BOTKA; Miroslav ŠOÓŠ a Roman PANTŮČEK. Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus. In XXXIV. konference mladých mikrobiologů - TOMÁŠKOVY DNY 2025. 2025. ISBN 978-80-280-0776-8.

@proceedings{2532771,
   author = {Komárková, Marie and Benešík, Martin and Procházková, Tereza and Vinco, Adam and Laichmanová, Monika and Smetanová, Soňa and Jelínek, Petr and Moša, Marek and Botka, Tibor and Šoóš, Miroslav and Pantůček, Roman},
   booktitle = {XXXIV. konference mladých mikrobiologů - TOMÁŠKOVY DNY 2025},
   keywords = {phage therapy; Kayvirus; Pbunavirus},
   language = {eng},
   isbn = {978-80-280-0776-8},
   title = {Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus},
   url = {https://doi.org/10.5817/CZ.MUNI.P280-0776-2025},
   year = {2025}
}

TY  - CONF
ID  - 2532771
AU  - Komárková, Marie - Benešík, Martin - Procházková, Tereza - Vinco, Adam - Laichmanová, Monika - Smetanová, Soňa - Jelínek, Petr - Moša, Marek - Botka, Tibor - Šoóš, Miroslav - Pantůček, Roman
PY  - 2025
TI  - Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus
SN  - 9788028007768
KW  - phage therapy
KW  - Kayvirus
KW  - Pbunavirus
UR  - https://doi.org/10.5817/CZ.MUNI.P280-0776-2025
N2  - Antimicrobial preservatives can be used to maintain the microbiological quality of phage preparations, but due to their potential antiviral effect, they may have an adverse influence on phage activity. Moreover, there is a possibility of physicochemical incompatibilities between phage particles and the preservatives. These phenomena may disrupt the effectiveness and quality of the final dosage form containing phages as an active ingredient. For this reason, the subject of this study is the compatibility of antimicrobial preservatives with therapeutic phages in liquid dosage form. We evaluated the stability of phage activity after exposure to 12 preservatives selected from various chemical classes by the double-layer agar method. Staphylococcal phage DSM 33473 of the genus Kayvirus and the pseudomonal phage DSM 33593 of the genus Pbunavirus, with a 9 log PFU/ml titer purified into physiological saline solution, were tested. The influence of the phages on the effect of the preservatives was determined by the microdilution method on Escherichia coli CCM 4517, Candida albicans CCM 8215 and Aspergillus brasiliensis CCM 8222. Physicochemical incompatibilities were evaluated by dynamic light scattering analysis. The assessed preservatives were classified into suitable, moderately suitable and unsuitable for manufacturing phage preparations. The suitable preservatives with slight reduction in phage titre were m-cresol, phenylethyl alcohol, sodium benzoate and sodium propionate. The unsuitable were chlorhexidine digluconate, benzalkonium chloride, thimerosal and diazolidinylurea. Chlorhexidine precipitated in the absence of phages, while benzalkonium only in the presence of phages. Thimerosal caused a decrease of 7 log PFU/ml only in DSM 33593. Diazolidinylurea led to a complete loss of titer in both genera. The result of this work is a classification of preservatives that can be used to optimise dosage forms with bacteriophages and ensure that they do not pose risks to patients due to microbial contamination or phage degradation caused by excipients. The study was supported by the National Institute of Virology and Bacteriology (LX22NPO5103) - Funded by the EU - NGEU. The students wish to thank the GA MU for its support (MUNI/A/1603/2024).
ER  -

KOMÁRKOVÁ, Marie; Martin BENEŠÍK; Tereza PROCHÁZKOVÁ; Adam VINCO; Monika LAICHMANOVÁ; Soňa SMETANOVÁ; Petr JELÍNEK; Marek MOŠA; Tibor BOTKA; Miroslav ŠOÓŠ a Roman PANTŮČEK. Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus. In \textit{XXXIV. konference mladých mikrobiologů - TOMÁŠKOVY DNY 2025}. 2025. ISBN~978-80-280-0776-8.

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