2025
Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence
MÁDI, Lenka; Josef KUČERA; Ondřej PEŠ; Pavel ŠMAK; Jakub MARTIKAN et al.Základní údaje
Originální název
Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence
Autoři
Vydání
Microchemical Journal, Elsevier, 2025, 0026-265X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10406 Analytical chemistry
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 5.100 v roce 2024
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/25:00142583
Organizační jednotka
Přírodovědecká fakulta
UT WoS
EID Scopus
Klíčová slova anglicky
HPLC; Tetracycline antibiotics; Fluorescence detection; Heavy water; Fluorescence enhancement; Quenching
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 17. 12. 2025 14:33, Mgr. Pavla Foltynová, Ph.D.
Anotace
V originále
The fluorescence properties of tetracycline antibiotics (tetracycline, minocycline, doxycycline, and omadacycline) were investigated in water and heavy water to evaluate the influence of solvent effects and the presence of metal ions on their spectral behavior. Emission spectra were recorded, both in the absence and presence of divalent metal cations (Mg2+, Ca2+) and the monovalent Li+ ion. All compounds exhibited enhanced fluorescence in D₂O compared to H₂O, with a marked increase upon the addition of Mg2+ ions. Emission maxima shifted in the presence of Mg2+, and fluorescence intensities reached up to 34.5-fold higher for omadacycline in D₂O with Mg2+. Li+ showed no significant effect due to its weak coordination properties; however, fluorescence enhancement was still observed in D₂O, indicating the sole influence of solvent even in the absence of strong complex formation. Chromatographic separation was successfully achieved for tetracycline, minocycline, and omadacycline using HPLC coupled with fluorescence detection. The combined use of D₂O and Mg2+ not only improved detection sensitivity but also lowered limits of detection (LODs), reaching as low as 3.1 · 10−9 mol·L−1 for omadacycline. These findings highlight the cumulative effects of solvent and metal ion environments on tetracycline fluorescence and provide valuable insights for developing sensitive analytical methods for antibiotic detection in pharmaceutical, clinical, and environmental samples.
Návaznosti
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