DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN 
ANALYSIS

k 2025

DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS

JAMRICHOVÁ, Jaroslava; Pavel BOBÁĽ a Hana PÍŽOVÁ

Základní údaje

Originální název

DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS

Autoři

JAMRICHOVÁ, Jaroslava; Pavel BOBÁĽ a Hana PÍŽOVÁ

Vydání

53rd Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30104 Pharmacology and pharmacy

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Organizační jednotka

Farmaceutická fakulta

ISBN

978-80-280-0773-7

Klíčová slova anglicky

BODIPY; FLUORESCENT TAGS; GLYCAN ANALYSIS; HYDRAZONE

Štítky

BODIPY, ÚChL

Změněno: 27. 1. 2026 12:56, Mgr. Jaroslava Jamrichová

Anotace

V originále

Protein glycosylation and the impact of glycan structures on their function represent a crucial area of research in disease diagnostics and the analysis of pathogenic mechanisms. However, glycan analysis is challenging due to their structural variability and the limited sensitivity of existing analytical methods. Therefore, a combination of fluorescence detection and mass spectrometry (MS) is often employed [1]. We focused on the development of novel fluorophore derivatives containing sulfonic and cationic groups, enhancing their solubility in aqueous solutions and improving glycan detection sensitivity. These modifications are based on established skeletal structures such as BODIPY. Additionally, a hydrazine moiety is incorporated to facilitate stable binding with oligosaccharides through hydrazone chemistry. This approach offers higher reactivity with carbonyl-containing carbohydrate compounds, ensuring the formation of stable complexes suitable for fluorescence detection and MS analysis. The hydrazinefunctionalized fluorophores are synthesized via "click" chemistry to ensure compatibility and synthetic efficiency. The developed labeling methods provide an innovative approach for more sensitive and accurate glycan analysis, contributing to a better understanding of biological processes and disease diagnostics.

Návaznosti

MUNI/A/1497/2024, interní kód MU

Název: Design a syntéza kladně a záporně nabitých vodorozpustných fluorescenčních značek pro detekci oligosacharidů

Investor: Masarykova univerzita, Design a syntéza kladně a záporně nabitých vodorozpustných fluorescenčních značek pro detekci oligosacharidů

Citovat

JAMRICHOVÁ, Jaroslava; Pavel BOBÁĽ a Hana PÍŽOVÁ. DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS. In 53rd Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2025. 2025. ISBN 978-80-280-0773-7.

@proceedings{2533807,
   author = {Jamrichová, Jaroslava and Bobáľ, Pavel and Pížová, Hana},
   booktitle = {53rd Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2025},
   keywords = {BODIPY; FLUORESCENT TAGS; GLYCAN ANALYSIS; HYDRAZONE},
   language = {eng},
   isbn = {978-80-280-0773-7},
   title = {DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS},
   year = {2025}
}

TY  - CONF
ID  - 2533807
AU  - Jamrichová, Jaroslava - Bobáľ, Pavel - Pížová, Hana
PY  - 2025
TI  - DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS
SN  - 9788028007737
KW  - BODIPY
KW  - FLUORESCENT TAGS
KW  - GLYCAN ANALYSIS
KW  - HYDRAZONE
N2  - Protein glycosylation and the impact of glycan structures on their function represent a crucial area of research in disease diagnostics and the analysis of pathogenic mechanisms. However, glycan analysis is challenging due to their structural variability and the limited sensitivity of existing analytical methods. Therefore, a combination of fluorescence detection and mass spectrometry (MS) is often employed [1]. We focused on the development of novel fluorophore derivatives containing sulfonic and cationic groups, enhancing their solubility in aqueous solutions and improving glycan detection sensitivity. These modifications are based on established skeletal structures such as BODIPY. Additionally, a hydrazine moiety is incorporated to facilitate stable binding with oligosaccharides through hydrazone chemistry. This approach offers higher reactivity with carbonyl-containing carbohydrate compounds, ensuring the formation of stable complexes suitable for fluorescence detection and MS analysis. The hydrazinefunctionalized fluorophores are synthesized via "click" chemistry to ensure compatibility and synthetic efficiency. The developed labeling methods provide an innovative approach for more sensitive and accurate glycan analysis, contributing to a better understanding of biological processes and disease diagnostics.
ER  -

JAMRICHOVÁ, Jaroslava; Pavel BOBÁĽ a Hana PÍŽOVÁ. DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS. In \textit{53rd Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2025}. 2025. ISBN~978-80-280-0773-7.

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